TY - JOUR
T1 - Detection and identification of labeled DNA by surface enhanced resonance Raman scattering
AU - Graham, D.
AU - Mallinder, B.J.
AU - Smith, W.E.
PY - 2000
Y1 - 2000
N2 - The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.
AB - The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.
KW - labeled DNA
KW - surface enhanced resonance Raman scattering
UR - http://dx.doi.org/10.1002/(SICI)1097-0282(2000)57:2<85::AID-BIP5>3.0.CO;2-#
U2 - 10.1002/(SICI)1097-0282(2000)57:2<85::AID-BIP5>3.0.CO;2-#
DO - 10.1002/(SICI)1097-0282(2000)57:2<85::AID-BIP5>3.0.CO;2-#
M3 - Article
SN - 0006-3525
VL - 57
SP - 85
EP - 91
JO - Biopolymers
JF - Biopolymers
IS - 2
ER -