Detection and identification of labeled DNA by surface enhanced resonance Raman scattering

D. Graham, B.J. Mallinder, W.E. Smith

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.
LanguageEnglish
Pages85-91
Number of pages6
JournalBiopolymers
Volume57
Issue number2
DOIs
Publication statusPublished - 2000

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Raman Spectrum Analysis
Raman scattering
DNA
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Keywords

  • labeled DNA
  • surface enhanced resonance Raman scattering

Cite this

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Detection and identification of labeled DNA by surface enhanced resonance Raman scattering. / Graham, D.; Mallinder, B.J.; Smith, W.E.

In: Biopolymers, Vol. 57, No. 2, 2000, p. 85-91.

Research output: Contribution to journalArticle

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AU - Graham, D.

AU - Mallinder, B.J.

AU - Smith, W.E.

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AB - The detection of specific sequences of DNA bases in a single strand can be achieved by hybridization of a known sequence of synthetic DNA. Due to the low concentrations usually used, a fluorescent label is required to detect the probe. Surface enhanced resonance Raman scattering (SERRS) also has the required sensitivity and provides a specific set of signals that are more applicable to discrimination of a number of probes without separation. A reliable SERRS method is reported here using two probes specifically designed for SERRS. It was possible to detect a 2 x 10(-12)M solution of labeled DNA, which illustrated the sensitive nature of SERRS for DNA analysis.

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KW - surface enhanced resonance Raman scattering

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