Detecting beta-amyloid glycation by intrinsic fluorescence - understanding the link between diabetes and Alzheimer's disease

Abeer Alghamdi, Shareen Forbes, David J.S. Birch, Vladislav Vyshemirsky, Olaf J. Rolinski

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

We monitor early stages of beta-amyloid (Aβ 1–40) aggregation, one of the key processes leading to Alzheimer's disease (AD), in the presence of high glucose concentrations by measuring Aβ 1– 40 intrinsic fluorescence. The multiple peaks and their shifts observed in the time-resolved emission spectra (TRES) reveal the impact of glycation on Aβ 1– 40 oligomerisation. The results show that formation of the advanced glycation end products (AGEs) alters the aggregation pathway. These changes are highly relevant to our understanding of the pathophysiology of AD and the implication of AGE and diabetes in these pathways.

Original languageEnglish
Article number108886
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume704
Early online date19 Apr 2021
DOIs
Publication statusPublished - 15 Jun 2021

Keywords

  • beta-amyloid aggregation
  • time-resolved emission spectra
  • glycation
  • tyrosine fluorescence decay
  • Alzheimer’s disease

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