Detecting beta-amyloid glycation by intrinsic fluorescence - understanding the link between diabetes and Alzheimer's disease

Abeer Alghamdi; Shareen Forbes; David J.S. Birch; Vladislav Vyshemirsky; Olaf J. Rolinski

doi:10.1016/j.abb.2021.108886

Detecting beta-amyloid glycation by intrinsic fluorescence - understanding the link between diabetes and Alzheimer's disease

Abeer Alghamdi, Shareen Forbes, David J.S. Birch, Vladislav Vyshemirsky, Olaf J. Rolinski

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Abstract

We monitor early stages of beta-amyloid (Aβ _1–40) aggregation, one of the key processes leading to Alzheimer's disease (AD), in the presence of high glucose concentrations by measuring Aβ _{1– 40} intrinsic fluorescence. The multiple peaks and their shifts observed in the time-resolved emission spectra (TRES) reveal the impact of glycation on Aβ _{1– 40} oligomerisation. The results show that formation of the advanced glycation end products (AGEs) alters the aggregation pathway. These changes are highly relevant to our understanding of the pathophysiology of AD and the implication of AGE and diabetes in these pathways.

Original language	English
Article number	108886
Number of pages	8
Journal	Archives of Biochemistry and Biophysics
Volume	704
Early online date	19 Apr 2021
DOIs	https://doi.org/10.1016/j.abb.2021.108886
Publication status	Published - 15 Jun 2021

Keywords

beta-amyloid aggregation
time-resolved emission spectra
glycation
tyrosine fluorescence decay
Alzheimer’s disease

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10.1016/j.abb.2021.108886

Alghamdi-etal-ABB-2021-Detecting-beta-amyloid-glycation-by-intrinsic-fluorescenceAccepted author manuscript, 864 KBLicence: CC BY-NC-ND 4.0

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title = "Detecting beta-amyloid glycation by intrinsic fluorescence - understanding the link between diabetes and Alzheimer's disease",

abstract = "We monitor early stages of beta-amyloid (Aβ 1–40) aggregation, one of the key processes leading to Alzheimer's disease (AD), in the presence of high glucose concentrations by measuring Aβ 1– 40 intrinsic fluorescence. The multiple peaks and their shifts observed in the time-resolved emission spectra (TRES) reveal the impact of glycation on Aβ 1– 40 oligomerisation. The results show that formation of the advanced glycation end products (AGEs) alters the aggregation pathway. These changes are highly relevant to our understanding of the pathophysiology of AD and the implication of AGE and diabetes in these pathways. ",

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T1 - Detecting beta-amyloid glycation by intrinsic fluorescence - understanding the link between diabetes and Alzheimer's disease

AU - Alghamdi, Abeer

AU - Forbes, Shareen

AU - Birch, David J.S.

AU - Vyshemirsky, Vladislav

AU - Rolinski, Olaf J.

PY - 2021/6/15

Y1 - 2021/6/15

N2 - We monitor early stages of beta-amyloid (Aβ 1–40) aggregation, one of the key processes leading to Alzheimer's disease (AD), in the presence of high glucose concentrations by measuring Aβ 1– 40 intrinsic fluorescence. The multiple peaks and their shifts observed in the time-resolved emission spectra (TRES) reveal the impact of glycation on Aβ 1– 40 oligomerisation. The results show that formation of the advanced glycation end products (AGEs) alters the aggregation pathway. These changes are highly relevant to our understanding of the pathophysiology of AD and the implication of AGE and diabetes in these pathways.

AB - We monitor early stages of beta-amyloid (Aβ 1–40) aggregation, one of the key processes leading to Alzheimer's disease (AD), in the presence of high glucose concentrations by measuring Aβ 1– 40 intrinsic fluorescence. The multiple peaks and their shifts observed in the time-resolved emission spectra (TRES) reveal the impact of glycation on Aβ 1– 40 oligomerisation. The results show that formation of the advanced glycation end products (AGEs) alters the aggregation pathway. These changes are highly relevant to our understanding of the pathophysiology of AD and the implication of AGE and diabetes in these pathways.

KW - beta-amyloid aggregation

KW - time-resolved emission spectra

KW - glycation

KW - tyrosine fluorescence decay

KW - Alzheimer’s disease

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DO - 10.1016/j.abb.2021.108886

M3 - Article

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VL - 704

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

M1 - 108886

ER -

Detecting beta-amyloid glycation by intrinsic fluorescence - understanding the link between diabetes and Alzheimer's disease

Abstract

Keywords

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