Detecting beta-amyloid aggregation from the time-resolved emission spectra

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The aggregation of beta-amyloids is one of the key processes responsible for the development of Alzheimer's disease. Early molecular-level detection of beta-amyloid oligomers may help in early diagnosis and in the development of new intervention therapies. Our previous studies on the changes in beta-amyloid's single tyrosine intrinsic fluorescence response during aggregation demonstrated a four-exponential fluorescence intensity decay, and the ratio of the pre-exponential factors indicated the extent of the aggregation in the early stages of the process before the beta-sheets were formed. Here we present a complementary approach based on the time-resolved emission spectra (TRES) of
amyloid's tyrosine excited at 279 nmand fluorescent in the window 240–450 nm. TRES has been used to demonstrate sturctural changes occuring on the nanosecond time scale after excitation which has significant advantages over using steady-state spectra.Wedemonstrate this by resolving the fluorescent species and revealing that beta-amyloid's monomers show very fast dielectric relaxation, and its oligomers display a substantial spectral shift due to dielectric relaxation, which gradually decreases when the oligomers become larger.
LanguageEnglish
Number of pages6
JournalMethods and Applications in Fluorescence
Early online date6 Dec 2017
DOIs
Publication statusE-pub ahead of print - 6 Dec 2017

Fingerprint

oligomers
Amyloid
emission spectra
Agglomeration
tyrosine
Oligomers
Dielectric relaxation
Tyrosine
fluorescence
Fluorescence
therapy
monomers
Monomers
shift
decay
excitation

Keywords

  • Alzheimer's disease
  • beta-amyloids
  • diagnosis

Cite this

@article{292bb7405aad4a66bd2426654a28718c,
title = "Detecting beta-amyloid aggregation from the time-resolved emission spectra",
abstract = "The aggregation of beta-amyloids is one of the key processes responsible for the development of Alzheimer's disease. Early molecular-level detection of beta-amyloid oligomers may help in early diagnosis and in the development of new intervention therapies. Our previous studies on the changes in beta-amyloid's single tyrosine intrinsic fluorescence response during aggregation demonstrated a four-exponential fluorescence intensity decay, and the ratio of the pre-exponential factors indicated the extent of the aggregation in the early stages of the process before the beta-sheets were formed. Here we present a complementary approach based on the time-resolved emission spectra (TRES) ofamyloid's tyrosine excited at 279 nmand fluorescent in the window 240–450 nm. TRES has been used to demonstrate sturctural changes occuring on the nanosecond time scale after excitation which has significant advantages over using steady-state spectra.Wedemonstrate this by resolving the fluorescent species and revealing that beta-amyloid's monomers show very fast dielectric relaxation, and its oligomers display a substantial spectral shift due to dielectric relaxation, which gradually decreases when the oligomers become larger.",
keywords = "Alzheimer's disease, beta-amyloids, diagnosis",
author = "Abeer Alghamdi and Vladislav Vyshemirsky and Birch, {David J S} and Rolinski, {Olaf J}",
note = "This is an author-created, un-copyedited version of an article accepted for publication in Methods and Applications in Fluorescence . IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/2050-6120/aa9f95.",
year = "2017",
month = "12",
day = "6",
doi = "10.1088/2050-6120/aa9f95",
language = "English",
journal = "Methods and Applications in Fluorescence",
issn = "2050-6120",

}

TY - JOUR

T1 - Detecting beta-amyloid aggregation from the time-resolved emission spectra

AU - Alghamdi, Abeer

AU - Vyshemirsky, Vladislav

AU - Birch, David J S

AU - Rolinski, Olaf J

N1 - This is an author-created, un-copyedited version of an article accepted for publication in Methods and Applications in Fluorescence . IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/2050-6120/aa9f95.

PY - 2017/12/6

Y1 - 2017/12/6

N2 - The aggregation of beta-amyloids is one of the key processes responsible for the development of Alzheimer's disease. Early molecular-level detection of beta-amyloid oligomers may help in early diagnosis and in the development of new intervention therapies. Our previous studies on the changes in beta-amyloid's single tyrosine intrinsic fluorescence response during aggregation demonstrated a four-exponential fluorescence intensity decay, and the ratio of the pre-exponential factors indicated the extent of the aggregation in the early stages of the process before the beta-sheets were formed. Here we present a complementary approach based on the time-resolved emission spectra (TRES) ofamyloid's tyrosine excited at 279 nmand fluorescent in the window 240–450 nm. TRES has been used to demonstrate sturctural changes occuring on the nanosecond time scale after excitation which has significant advantages over using steady-state spectra.Wedemonstrate this by resolving the fluorescent species and revealing that beta-amyloid's monomers show very fast dielectric relaxation, and its oligomers display a substantial spectral shift due to dielectric relaxation, which gradually decreases when the oligomers become larger.

AB - The aggregation of beta-amyloids is one of the key processes responsible for the development of Alzheimer's disease. Early molecular-level detection of beta-amyloid oligomers may help in early diagnosis and in the development of new intervention therapies. Our previous studies on the changes in beta-amyloid's single tyrosine intrinsic fluorescence response during aggregation demonstrated a four-exponential fluorescence intensity decay, and the ratio of the pre-exponential factors indicated the extent of the aggregation in the early stages of the process before the beta-sheets were formed. Here we present a complementary approach based on the time-resolved emission spectra (TRES) ofamyloid's tyrosine excited at 279 nmand fluorescent in the window 240–450 nm. TRES has been used to demonstrate sturctural changes occuring on the nanosecond time scale after excitation which has significant advantages over using steady-state spectra.Wedemonstrate this by resolving the fluorescent species and revealing that beta-amyloid's monomers show very fast dielectric relaxation, and its oligomers display a substantial spectral shift due to dielectric relaxation, which gradually decreases when the oligomers become larger.

KW - Alzheimer's disease

KW - beta-amyloids

KW - diagnosis

UR - http://iopscience.iop.org/journal/2050-6120

U2 - 10.1088/2050-6120/aa9f95

DO - 10.1088/2050-6120/aa9f95

M3 - Article

JO - Methods and Applications in Fluorescence

T2 - Methods and Applications in Fluorescence

JF - Methods and Applications in Fluorescence

SN - 2050-6120

ER -