Designing peptide actuators for enzyme-responsive particles

Rein V. Ulijn, Robert J. Mart, Thomas T. McDonald, Paul D. Thornton

Research output: Contribution to journalMeeting abstract

Abstract

We will report on enzyme-responsive hydrogel particles for capture and controlled release applications. These particles are functionalised with peptide actuators that simultaneously act as biorecognition elements and molecular actuators, triggering swelling or collapse of particles in response to enzymes under constant, physiological conditions. Amino-functionalised poly(ethylene glycol acrylamide) (PEGA) hydrogel particles were functionalised with peptide actuators using solid-phase synthesis approach directly on the polymer material. We demonstrate that these enzyme responsive particles can be designed to (i) achieve an increase or decrease of molecular accessibility of the polymer, as required; (ii) match the specificity of the target enzyme; (iii) match the size and charge properties of the to-be released protein payload, thereby uniquely allowing for tuneable release profiles and (iv) match the ionic strength of the environment. Release profiles were analysed using a combination of fluorescence spectroscopy of the solution and two-photon fluorescence microscopy to analyse enzymatically triggered molecular events within hydrogel particles during the initial stages of release.
LanguageEnglish
Number of pages1
JournalAbstracts of papers - American Chemical Society
Volume238
Publication statusPublished - 16 Aug 2009

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Peptides
Particles (particulate matter)
Hydrogel
Actuators
Enzymes
Hydrogels
Polymers
Acrylamide
Fluorescence microscopy
Fluorescence spectroscopy
Ionic strength
Polyethylene glycols
Swelling
Photons
Proteins

Keywords

  • design
  • peptide actuators
  • enzyme-responsive particles

Cite this

Ulijn, Rein V. ; Mart, Robert J. ; McDonald, Thomas T. ; Thornton, Paul D. / Designing peptide actuators for enzyme-responsive particles. In: Abstracts of papers - American Chemical Society. 2009 ; Vol. 238.
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Designing peptide actuators for enzyme-responsive particles. / Ulijn, Rein V.; Mart, Robert J.; McDonald, Thomas T.; Thornton, Paul D.

In: Abstracts of papers - American Chemical Society, Vol. 238, 16.08.2009.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - Designing peptide actuators for enzyme-responsive particles

AU - Ulijn, Rein V.

AU - Mart, Robert J.

AU - McDonald, Thomas T.

AU - Thornton, Paul D.

PY - 2009/8/16

Y1 - 2009/8/16

N2 - We will report on enzyme-responsive hydrogel particles for capture and controlled release applications. These particles are functionalised with peptide actuators that simultaneously act as biorecognition elements and molecular actuators, triggering swelling or collapse of particles in response to enzymes under constant, physiological conditions. Amino-functionalised poly(ethylene glycol acrylamide) (PEGA) hydrogel particles were functionalised with peptide actuators using solid-phase synthesis approach directly on the polymer material. We demonstrate that these enzyme responsive particles can be designed to (i) achieve an increase or decrease of molecular accessibility of the polymer, as required; (ii) match the specificity of the target enzyme; (iii) match the size and charge properties of the to-be released protein payload, thereby uniquely allowing for tuneable release profiles and (iv) match the ionic strength of the environment. Release profiles were analysed using a combination of fluorescence spectroscopy of the solution and two-photon fluorescence microscopy to analyse enzymatically triggered molecular events within hydrogel particles during the initial stages of release.

AB - We will report on enzyme-responsive hydrogel particles for capture and controlled release applications. These particles are functionalised with peptide actuators that simultaneously act as biorecognition elements and molecular actuators, triggering swelling or collapse of particles in response to enzymes under constant, physiological conditions. Amino-functionalised poly(ethylene glycol acrylamide) (PEGA) hydrogel particles were functionalised with peptide actuators using solid-phase synthesis approach directly on the polymer material. We demonstrate that these enzyme responsive particles can be designed to (i) achieve an increase or decrease of molecular accessibility of the polymer, as required; (ii) match the specificity of the target enzyme; (iii) match the size and charge properties of the to-be released protein payload, thereby uniquely allowing for tuneable release profiles and (iv) match the ionic strength of the environment. Release profiles were analysed using a combination of fluorescence spectroscopy of the solution and two-photon fluorescence microscopy to analyse enzymatically triggered molecular events within hydrogel particles during the initial stages of release.

KW - design

KW - peptide actuators

KW - enzyme-responsive particles

M3 - Meeting abstract

VL - 238

JO - Abstracts of papers - American Chemical Society

T2 - Abstracts of papers - American Chemical Society

JF - Abstracts of papers - American Chemical Society

SN - 0065-7727

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