Delivery of the antibiotic gentamicin sulphate from precipitation cast matrices of polycaprolactone

H.-I. Chang, Y. Perrie, A.G.A. Coombes

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Microporous, poly(ε-caprolactone) (PCL) matrices were loaded with the aminoglycoside antibiotic, gentamicin sulphate (GS) using the precipitation casting technique by suspension of powder in the PCL solution prior to casting. Improvements in drug loading from 1.8% to 6.7% w/w and distribution in the matrices were obtained by pre-cooling the suspension to 4 °C. Gradual release of approximately 80% of the GS content occurred over 11 weeks in PBS at 37 °C and low amounts of antibiotic were measured up to 20 weeks. The kinetics of release could be described effectively by the Higuchi model with the diffusion rate constant (D) increasing from of 1.7 to 5.1 μg/mg matrix/day0.5 as the drug loading increased from 1.4% to 8.3% w/w. GS-loaded PCL matrices retained anti-bacterial activity after immersion in PBS at 37 °C over 14 days as demonstrated by inhibition of growth of S. epidermidis in culture. These findings recommend further investigation of precipitation-cast PCL matrices for delivery of hydrophilic molecules such as anti-bacterial agents from implanted, inserted or topical devices.
Original languageEnglish
Pages (from-to)414-421
Number of pages8
JournalJournal of Controlled Release
Volume110
Issue number2
DOIs
Publication statusPublished - 10 Jan 2006

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Gentamicins
Anti-Bacterial Agents
Suspensions
Aminoglycosides
Immersion
Pharmaceutical Preparations
Powders
Equipment and Supplies
Growth
polycaprolactone

Keywords

  • microporous
  • polycaprolactone
  • antibiotics
  • infection control

Cite this

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title = "Delivery of the antibiotic gentamicin sulphate from precipitation cast matrices of polycaprolactone",
abstract = "Microporous, poly(ε-caprolactone) (PCL) matrices were loaded with the aminoglycoside antibiotic, gentamicin sulphate (GS) using the precipitation casting technique by suspension of powder in the PCL solution prior to casting. Improvements in drug loading from 1.8{\%} to 6.7{\%} w/w and distribution in the matrices were obtained by pre-cooling the suspension to 4 °C. Gradual release of approximately 80{\%} of the GS content occurred over 11 weeks in PBS at 37 °C and low amounts of antibiotic were measured up to 20 weeks. The kinetics of release could be described effectively by the Higuchi model with the diffusion rate constant (D) increasing from of 1.7 to 5.1 μg/mg matrix/day0.5 as the drug loading increased from 1.4{\%} to 8.3{\%} w/w. GS-loaded PCL matrices retained anti-bacterial activity after immersion in PBS at 37 °C over 14 days as demonstrated by inhibition of growth of S. epidermidis in culture. These findings recommend further investigation of precipitation-cast PCL matrices for delivery of hydrophilic molecules such as anti-bacterial agents from implanted, inserted or topical devices.",
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author = "H.-I. Chang and Y. Perrie and A.G.A. Coombes",
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Delivery of the antibiotic gentamicin sulphate from precipitation cast matrices of polycaprolactone. / Chang, H.-I.; Perrie, Y.; Coombes, A.G.A.

In: Journal of Controlled Release, Vol. 110, No. 2, 10.01.2006, p. 414-421.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Chang, H.-I.

AU - Perrie, Y.

AU - Coombes, A.G.A.

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N2 - Microporous, poly(ε-caprolactone) (PCL) matrices were loaded with the aminoglycoside antibiotic, gentamicin sulphate (GS) using the precipitation casting technique by suspension of powder in the PCL solution prior to casting. Improvements in drug loading from 1.8% to 6.7% w/w and distribution in the matrices were obtained by pre-cooling the suspension to 4 °C. Gradual release of approximately 80% of the GS content occurred over 11 weeks in PBS at 37 °C and low amounts of antibiotic were measured up to 20 weeks. The kinetics of release could be described effectively by the Higuchi model with the diffusion rate constant (D) increasing from of 1.7 to 5.1 μg/mg matrix/day0.5 as the drug loading increased from 1.4% to 8.3% w/w. GS-loaded PCL matrices retained anti-bacterial activity after immersion in PBS at 37 °C over 14 days as demonstrated by inhibition of growth of S. epidermidis in culture. These findings recommend further investigation of precipitation-cast PCL matrices for delivery of hydrophilic molecules such as anti-bacterial agents from implanted, inserted or topical devices.

AB - Microporous, poly(ε-caprolactone) (PCL) matrices were loaded with the aminoglycoside antibiotic, gentamicin sulphate (GS) using the precipitation casting technique by suspension of powder in the PCL solution prior to casting. Improvements in drug loading from 1.8% to 6.7% w/w and distribution in the matrices were obtained by pre-cooling the suspension to 4 °C. Gradual release of approximately 80% of the GS content occurred over 11 weeks in PBS at 37 °C and low amounts of antibiotic were measured up to 20 weeks. The kinetics of release could be described effectively by the Higuchi model with the diffusion rate constant (D) increasing from of 1.7 to 5.1 μg/mg matrix/day0.5 as the drug loading increased from 1.4% to 8.3% w/w. GS-loaded PCL matrices retained anti-bacterial activity after immersion in PBS at 37 °C over 14 days as demonstrated by inhibition of growth of S. epidermidis in culture. These findings recommend further investigation of precipitation-cast PCL matrices for delivery of hydrophilic molecules such as anti-bacterial agents from implanted, inserted or topical devices.

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