Deletion of the gene for the membrane-bound acid phosphatase of Leishmania mexicana

I Benzel, F Weise, M Wiese

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The membrane-bound acid phosphatase of Leishmania mexicana (LmxMBAP) has been shown to be a heterogeneously N-glycosylated type I transmembrane protein, which is localized predominantly in vesicular structures close to the flagellar pocket in promastigotes and amastigotes. Its expression in both life stages prompted us to analyse its function by performing deletion analysis. Both alleles of the single copy gene were sequentially replaced by resistance marker genes and the resulting deletion mutant was tested for its potential to infect Balb/c mice and peritoneal macrophages. There was no obvious difference detectable between the mutant and the wild-type. Therefore, we conclude that LmxMBAP is neither involved in the infection process nor required for amastigote survival in the infected host cell. LmxMBAP null mutant promastigotes were used to establish a system for homogeneous overexpression of LmxMBAP which will be useful to investigate protein sorting in L. mexicana.

LanguageEnglish
Pages77-86
Number of pages10
JournalMolecular and Biochemical Parasitology
Volume111
Issue number1
DOIs
Publication statusPublished - Nov 2000

Fingerprint

Leishmania mexicana
Gene Deletion
Acid Phosphatase
Membranes
Peritoneal Macrophages
Protein Transport
Alleles
Infection
Genes
Proteins

Keywords

  • acid phosphatase
  • alleles
  • cell membrane
  • gene deletion
  • gene targeting
  • genes, protozoan
  • genes, rRNA
  • Leishmania mexicana
  • Leishmaniasis, Cutaneous
  • macrophages
  • membrane proteins
  • mice, Inbred BALB C
  • molecular sequence data
  • protozoan proteins
  • transfection
  • virulence

Cite this

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title = "Deletion of the gene for the membrane-bound acid phosphatase of Leishmania mexicana",
abstract = "The membrane-bound acid phosphatase of Leishmania mexicana (LmxMBAP) has been shown to be a heterogeneously N-glycosylated type I transmembrane protein, which is localized predominantly in vesicular structures close to the flagellar pocket in promastigotes and amastigotes. Its expression in both life stages prompted us to analyse its function by performing deletion analysis. Both alleles of the single copy gene were sequentially replaced by resistance marker genes and the resulting deletion mutant was tested for its potential to infect Balb/c mice and peritoneal macrophages. There was no obvious difference detectable between the mutant and the wild-type. Therefore, we conclude that LmxMBAP is neither involved in the infection process nor required for amastigote survival in the infected host cell. LmxMBAP null mutant promastigotes were used to establish a system for homogeneous overexpression of LmxMBAP which will be useful to investigate protein sorting in L. mexicana.",
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Deletion of the gene for the membrane-bound acid phosphatase of Leishmania mexicana. / Benzel, I; Weise, F; Wiese, M.

In: Molecular and Biochemical Parasitology, Vol. 111, No. 1, 11.2000, p. 77-86.

Research output: Contribution to journalArticle

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AU - Weise, F

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