Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue

Ashleigh Holmes, Leighton Pritchard, Peter Hedley, Jenny Morris, Sean P. McAteer, David L. Gally, Nicola J. Holden

Research output: Contribution to journalArticle

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Abstract

A high-throughput positive-selection approach was taken to generate a dataset of Shigatoxigenic Escherichia coli (STEC) O157:H7 genes enriched in adherence to plant tissue. The approach generates a differential dataset based on BAC clones enriched in the output, after adherence, compared to the inoculum used as the input. A BAC clone library derived from STEC isolate 'Sakai' was used since this isolate is associated with a very large-scale outbreak of human disease from
consumption of contaminated fresh produce; white radish sprouts. Spinach was used for the screen since it is associated with STEC outbreaks, and the roots provide a suitable site for bacterial colonisation. Four successive of rounds of Sakai BAC clone selection and amplification were applied for spinach root adherence, in parallel to a non-plant control. Genomic DNA was obtained from a total of 7.17 x 108 cfu/ml of bacteria from the plant treatment and 1.13 x 109 cfu/ml of bacteria from the no-plant control. Relative gene abundance of the output compared to the input pools was obtained using an established E. coli DNA microarray chip for STEC. The dataset enables screening for genes enriched under the treatment condition and informs on genes that may play a role in plant-microbe interactions.
Original languageEnglish
Article number105769
Number of pages5
JournalData in Brief
Volume31
Early online date28 May 2020
DOIs
Publication statusPublished - 31 Aug 2020

Keywords

  • microarray
  • bacterial artificial chromosome
  • gene enrichment
  • high throughput screening
  • bacteria host interactions

Research Output

A high-throughput genomic screen identifies a role for the plasmid-borne type II secretion system of Escherichia coli O157:H7 (Sakai) in plant-microbe interactions

Holmes, A., Pritchard, L., Hedley, P. E., Morris, J., McAteer, S. P., Gally, D. L. & Holden, N. J., 12 Jul 2020, In : Genomics. 112, 6, p. 4242-4253 12 p.

Research output: Contribution to journalArticle

A high-throughput genomic screen identifies a role for the plasmid-borne type II secretion system of Escherichia coli O157:H7 (Sakai) in plant-microbe interactions

Holmes, A., Pritchard, L., Hedley, P., Morris, J., McAteer, S. P., Gally, D. L. & Holden, N. J., 13 Apr 2020, Cold Spring Harbor, N.Y., 30 p.

Research output: Working paper

Open Access
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Cite this

Holmes, A., Pritchard, L., Hedley, P., Morris, J., McAteer, S. P., Gally, D. L., & Holden, N. J. (2020). Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue. Data in Brief, 31, [105769]. https://doi.org/10.1016/j.dib.2020.105769