Cytotoxicity study of Ferula hermonis Boiss

Abdurrahim Elouzi, A.A. Auzi, M. El-Hammadi, A.I. Gray

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Ferula hermonis Boiss (Apiaceae) is known in the Midleast as 'zallouh'. In the last decade, scientists paid attention to study chemistry and biological activity of the plant. In this study Ferula hermonis were collected and evaluated for cytotoxic activity. The plant was extracted using different polarity solvents: petroleum ether, ethyl acetate and methanol. The cytotoxic effect of the extracts was examined in-vitro on human cell line (stomach cancer cell line, SCL-40). The assay was based on incubation of tetrazolium dye (MTT; a yellow water-soluble tetrazolium dye that is reduced by live, but not dead cells) with the cells that pre-incubated with a tested substance. A purple formazan product that produced can be determined spectrophotometrically. The result confirmed that the SCL-40 cells were much more sensitive to the petroleum extract than other extracts, which showed the prominent cytotoxicity. The plant showed pronounced cytotoxic activity will be further evaluated for the possible isolation of active antitumour compounds.
LanguageEnglish
Pages313-317
Number of pages4
JournalBulletin of Pharmaceutical Sciences
Volume31
Issue number2
Publication statusPublished - 2008

Fingerprint

Ferula
Coloring Agents
Apiaceae
Formazans
Cell Line
Petroleum
Stomach Neoplasms
Methanol
Water

Keywords

  • Ferula hermonis Boiss
  • Apiaceae
  • zallouh
  • chemistry
  • biological activity
  • plants
  • cytotoxic activity
  • polarity solvents
  • petroleum ether
  • ethyl acetate
  • methanol

Cite this

Elouzi, A., Auzi, A. A., El-Hammadi, M., & Gray, A. I. (2008). Cytotoxicity study of Ferula hermonis Boiss. Bulletin of Pharmaceutical Sciences, 31(2), 313-317.
Elouzi, Abdurrahim ; Auzi, A.A. ; El-Hammadi, M. ; Gray, A.I. / Cytotoxicity study of Ferula hermonis Boiss. In: Bulletin of Pharmaceutical Sciences. 2008 ; Vol. 31, No. 2. pp. 313-317.
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Elouzi, A, Auzi, AA, El-Hammadi, M & Gray, AI 2008, 'Cytotoxicity study of Ferula hermonis Boiss' Bulletin of Pharmaceutical Sciences, vol. 31, no. 2, pp. 313-317.

Cytotoxicity study of Ferula hermonis Boiss. / Elouzi, Abdurrahim; Auzi, A.A.; El-Hammadi, M.; Gray, A.I.

In: Bulletin of Pharmaceutical Sciences, Vol. 31, No. 2, 2008, p. 313-317.

Research output: Contribution to journalArticle

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T1 - Cytotoxicity study of Ferula hermonis Boiss

AU - Elouzi, Abdurrahim

AU - Auzi, A.A.

AU - El-Hammadi, M.

AU - Gray, A.I.

PY - 2008

Y1 - 2008

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AB - Ferula hermonis Boiss (Apiaceae) is known in the Midleast as 'zallouh'. In the last decade, scientists paid attention to study chemistry and biological activity of the plant. In this study Ferula hermonis were collected and evaluated for cytotoxic activity. The plant was extracted using different polarity solvents: petroleum ether, ethyl acetate and methanol. The cytotoxic effect of the extracts was examined in-vitro on human cell line (stomach cancer cell line, SCL-40). The assay was based on incubation of tetrazolium dye (MTT; a yellow water-soluble tetrazolium dye that is reduced by live, but not dead cells) with the cells that pre-incubated with a tested substance. A purple formazan product that produced can be determined spectrophotometrically. The result confirmed that the SCL-40 cells were much more sensitive to the petroleum extract than other extracts, which showed the prominent cytotoxicity. The plant showed pronounced cytotoxic activity will be further evaluated for the possible isolation of active antitumour compounds.

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Elouzi A, Auzi AA, El-Hammadi M, Gray AI. Cytotoxicity study of Ferula hermonis Boiss. Bulletin of Pharmaceutical Sciences. 2008;31(2):313-317.