Cytotoxic responses to 405 nm light exposure in mammalian and bacterial cells: involvement of reactive oxygen species

Praveen Ramakrishnan, Michelle Maclean, Scott J. MacGregor, John G. Anderson, M. Helen Grant

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29 Citations (Scopus)

Abstract

Light at wavelength 405 nm is an effective bactericide. Previous studies showed that exposing mammalian cells to 405 nm light at 36 J/cm2 (a bactericidal dose) had no significant effect on normal cell function, although at higher doses (54 J/cm2), mammalian cell death became evident. This research demonstrates that mammalian and bacterial cell toxicity induced by 405 nm light exposure is accompanied by reactive oxygen species production, as detected by generation of fluorescence from 6-carboxy-2’,7’- dichlorodihydrofluorescein diacetate. As indicators of the resulting oxidative stress in mammalian cells, a decrease in intracellular reduced glutathione content and a corresponding increase in the efflux of oxidised glutathione was observed from 405 nm light treated cells. The mammalian cells were significantly protected from dying at 54 J/cm2 in the presence of catalase, which detoxifies H2O2. Bacterial cells were significantly protected by sodium pyruvate (H2O2 scavenger) and by a combination of free radical scavengers (sodium pyruvate, dimethyl thiourea (OH scavenger) and catalase) at 162 and 324 J/cm2. Results herefore suggested that the cytotoxic mechanism of 405 nm light in mammalian cells and bacteria could be oxidative stress involving predominantly H2O2 generation, with other ROS contributing to the damage.
LanguageEnglish
Pages54-62
Number of pages8
JournalToxicology in Vitro
Volume33
Early online date23 Feb 2016
DOIs
Publication statusPublished - 1 Jun 2016

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Reactive Oxygen Species
Cells
Light
Oxygen
Oxidative stress
Pyruvic Acid
Catalase
Sodium
Bactericides
Thiourea
Free Radical Scavengers
Glutathione Disulfide
Thioureas
Cell death
Oxidative Stress
Free radicals
Dosimetry
Glutathione
Toxicity
Bacteria

Keywords

  • 405 nm light
  • cytotoxic mechanisms
  • bactericide
  • osteoblasts
  • reactive oxygen species
  • scavengers

Cite this

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title = "Cytotoxic responses to 405 nm light exposure in mammalian and bacterial cells: involvement of reactive oxygen species",
abstract = "Light at wavelength 405 nm is an effective bactericide. Previous studies showed that exposing mammalian cells to 405 nm light at 36 J/cm2 (a bactericidal dose) had no significant effect on normal cell function, although at higher doses (54 J/cm2), mammalian cell death became evident. This research demonstrates that mammalian and bacterial cell toxicity induced by 405 nm light exposure is accompanied by reactive oxygen species production, as detected by generation of fluorescence from 6-carboxy-2’,7’- dichlorodihydrofluorescein diacetate. As indicators of the resulting oxidative stress in mammalian cells, a decrease in intracellular reduced glutathione content and a corresponding increase in the efflux of oxidised glutathione was observed from 405 nm light treated cells. The mammalian cells were significantly protected from dying at 54 J/cm2 in the presence of catalase, which detoxifies H2O2. Bacterial cells were significantly protected by sodium pyruvate (H2O2 scavenger) and by a combination of free radical scavengers (sodium pyruvate, dimethyl thiourea (OH scavenger) and catalase) at 162 and 324 J/cm2. Results herefore suggested that the cytotoxic mechanism of 405 nm light in mammalian cells and bacteria could be oxidative stress involving predominantly H2O2 generation, with other ROS contributing to the damage.",
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author = "Praveen Ramakrishnan and Michelle Maclean and MacGregor, {Scott J.} and Anderson, {John G.} and Grant, {M. Helen}",
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AU - Grant, M. Helen

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