Cytotoxic metabolites from the fungal endophyte Alternaria sp. and their subsequent detection in its host plant Polygonum senegalense

Amal H. Aly, RuAngelie Edrada-Ebel, Ine Dewl Indriani, Victor Wray, Werner E.G. Muller, Frank Totzke, Ute Zirrglebel, Christoph Schachtele, Michael H.G. Kubbutat, W.H. Lin, Peter Proksch, Rainer Ebel

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Abstract

From the Egyptian medicinal plant Polygonum senegalense the fungal endophyte Alternaria sp. was isolated. Extracts of the fungus grown either in liquid culture or on solid rice media exhibited cytotoxic activity when tested in vitro against L5178Y cells. Chromatographic separation of the extracts yielded 15 natural products, out of which seven were new compounds, with both fungal extracts differing considerably with regard to their secondary metabolites. Compounds 1, 2, 3, 6, and 7 showed cytotoxic activity with EC50 values ranging from 1.7 to 7.8 µg/mL. When analyzed in vitro for their inhibitory potential against 24 different protein kinases, compounds 1−3, 5−8, and 15 inhibited several of these enzymes (IC50 values 0.22−9.8 µg/mL). Interestingly, compounds 1, 3, and 6 were also identified as constituents of an extract derived from healthy leaves of the host plant P. senegalense, thereby indicating that the production of natural products by the endophyte proceeds also under in situ conditions within the plant host.
Original languageEnglish
Pages (from-to)972-980
Number of pages8
JournalJournal of Natural Products
Volume71
Issue number6
DOIs
Publication statusPublished - May 2008

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Keywords

  • cytotoxic metabolites
  • fungal endophyte
  • alternaria sp
  • polygonum senegalense
  • plants
  • drug delivery
  • pharmacology
  • drugs

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