Abstract
| Original language | English |
|---|---|
| Number of pages | 9 |
| Journal | RSC Medicinal Chemistry |
| Early online date | 17 Dec 2019 |
| DOIs | |
| Publication status | E-pub ahead of print - 17 Dec 2019 |
Fingerprint
Keywords
- cyrene
- antibacterial drug discovery
- solvents
Cite this
}
Cyrene is a green alternative to DMSO as a solvent for antibacterial drug discovery against ESKAPE pathogens. / Camp, Jason E.; Nyamini, Simbarashe B.; Scott, Fraser J.
In: RSC Medicinal Chemistry, 17.12.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cyrene is a green alternative to DMSO as a solvent for antibacterial drug discovery against ESKAPE pathogens
AU - Camp, Jason E.
AU - Nyamini, Simbarashe B.
AU - Scott, Fraser J.
PY - 2019/12/17
Y1 - 2019/12/17
N2 - Dimethyl sulfoxide (DMSO) is currently employed across the biomedical field, from cryopreservation to in vitro assays, despite the fact that it has been shown to have an assortment of biologically relevant effects. The amphiphilic nature of DMSO along with its relatively low toxicity at dilute concentrations make it a challenging solvent to replace. A possible alternative is Cyrene™ (dihydrolevoglucosenone), an aprotic dipolar solvent that is derived from waste biomass. In addition to being a green solvent, Cyrene™ has comparable solvation properties and is reported to have low toxicity. Herein the abilities of the two solvents to solubilize drug compounds and to act as non-participatory vehicles in drug discovery for antibacterials are compared. It was demonstrate that the results of standardised antimicrobial susceptibility testing do not differ between drugs prepared from either Cyrene™ or DMSO stock. Moreover, in contrast to DMSO, Cyrene™ does not offer protection from ROS mediated killing of bacteria and may therefore be an improvement over DMSO as a vehicle in antimicrobial drug discovery.
AB - Dimethyl sulfoxide (DMSO) is currently employed across the biomedical field, from cryopreservation to in vitro assays, despite the fact that it has been shown to have an assortment of biologically relevant effects. The amphiphilic nature of DMSO along with its relatively low toxicity at dilute concentrations make it a challenging solvent to replace. A possible alternative is Cyrene™ (dihydrolevoglucosenone), an aprotic dipolar solvent that is derived from waste biomass. In addition to being a green solvent, Cyrene™ has comparable solvation properties and is reported to have low toxicity. Herein the abilities of the two solvents to solubilize drug compounds and to act as non-participatory vehicles in drug discovery for antibacterials are compared. It was demonstrate that the results of standardised antimicrobial susceptibility testing do not differ between drugs prepared from either Cyrene™ or DMSO stock. Moreover, in contrast to DMSO, Cyrene™ does not offer protection from ROS mediated killing of bacteria and may therefore be an improvement over DMSO as a vehicle in antimicrobial drug discovery.
KW - cyrene
KW - antibacterial drug discovery
KW - solvents
UR - https://www.rsc.org/journals-books-databases/about-journals/medchemcomm/
U2 - 10.0.4.15/C9MD00341J
DO - 10.0.4.15/C9MD00341J
M3 - Article
JO - RSC Medicinal Chemistry
JF - RSC Medicinal Chemistry
SN - 2040-2511
ER -