Current approaches to stabilising and analysing proteins during microencapsulation in PLGA

Christopher F. van der Walle, Gaurav Sharma, M.N.V Ravi Kumar

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Encapsulation of biomacromolecules in polyester micro- and nano-particles is now a routine procedure in many laboratories for achieving controlled and targeted delivery strategies. Objectives: Proteins are notoriously difficult to encapsulate without some degree of unfolding and loss of bioactivity, and this is despite around two decades of research. A case by case analysis appears necessary when determining which mechanism, generally unfolding at the emulsion interface or acidification of the particle interior, is most detrimental. The transient nature of the emulsion systems makes in situ, real-time analysis of interfacial events difficult, necessitating interpretation from model interfacial systems or analysis post-fabrication. The review will focus on: i) the emulsification efficiency of proteins, cf. interfacial adsorption; ii) current excipients and techniques used to stabilise proteins, outlining work towards the oral delivery of insulin as a case study; iii) analytical techniques used to characterise encapsulated protein. There appears to be a recent trend towards the stabilization of proteins via direct complexation with polymers but, in contrast, emulsion techniques are emerging which err away from the use of stabilisers and/or excipients. A number of spectroscopic and spectrometric methods have found new application to the study of protein integrity in microspheres.
LanguageEnglish
Pages177-186
Number of pages9
JournalExpert Opinion on Drug Delivery
Volume6
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Drug Compounding
Emulsions
Proteins
Excipients
Polyesters
Microspheres
Adsorption
polylactic acid-polyglycolic acid copolymer
Polymers
Insulin
Research

Keywords

  • biomacromolecules
  • polyester
  • nano-particles
  • drug delivery

Cite this

van der Walle, Christopher F. ; Sharma, Gaurav ; Kumar, M.N.V Ravi. / Current approaches to stabilising and analysing proteins during microencapsulation in PLGA. In: Expert Opinion on Drug Delivery. 2009 ; Vol. 6, No. 2. pp. 177-186.
@article{ac5b4bd49dcc47148534e54e7793c4ed,
title = "Current approaches to stabilising and analysing proteins during microencapsulation in PLGA",
abstract = "Encapsulation of biomacromolecules in polyester micro- and nano-particles is now a routine procedure in many laboratories for achieving controlled and targeted delivery strategies. Objectives: Proteins are notoriously difficult to encapsulate without some degree of unfolding and loss of bioactivity, and this is despite around two decades of research. A case by case analysis appears necessary when determining which mechanism, generally unfolding at the emulsion interface or acidification of the particle interior, is most detrimental. The transient nature of the emulsion systems makes in situ, real-time analysis of interfacial events difficult, necessitating interpretation from model interfacial systems or analysis post-fabrication. The review will focus on: i) the emulsification efficiency of proteins, cf. interfacial adsorption; ii) current excipients and techniques used to stabilise proteins, outlining work towards the oral delivery of insulin as a case study; iii) analytical techniques used to characterise encapsulated protein. There appears to be a recent trend towards the stabilization of proteins via direct complexation with polymers but, in contrast, emulsion techniques are emerging which err away from the use of stabilisers and/or excipients. A number of spectroscopic and spectrometric methods have found new application to the study of protein integrity in microspheres.",
keywords = "biomacromolecules, polyester, nano-particles, drug delivery",
author = "{van der Walle}, {Christopher F.} and Gaurav Sharma and Kumar, {M.N.V Ravi}",
year = "2009",
month = "2",
doi = "10.1517/17425240802680169",
language = "English",
volume = "6",
pages = "177--186",
journal = "Expert Opinion on Drug Delivery",
issn = "1742-5247",
number = "2",

}

Current approaches to stabilising and analysing proteins during microencapsulation in PLGA. / van der Walle, Christopher F.; Sharma, Gaurav; Kumar, M.N.V Ravi.

In: Expert Opinion on Drug Delivery, Vol. 6, No. 2, 02.2009, p. 177-186.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Current approaches to stabilising and analysing proteins during microencapsulation in PLGA

AU - van der Walle, Christopher F.

AU - Sharma, Gaurav

AU - Kumar, M.N.V Ravi

PY - 2009/2

Y1 - 2009/2

N2 - Encapsulation of biomacromolecules in polyester micro- and nano-particles is now a routine procedure in many laboratories for achieving controlled and targeted delivery strategies. Objectives: Proteins are notoriously difficult to encapsulate without some degree of unfolding and loss of bioactivity, and this is despite around two decades of research. A case by case analysis appears necessary when determining which mechanism, generally unfolding at the emulsion interface or acidification of the particle interior, is most detrimental. The transient nature of the emulsion systems makes in situ, real-time analysis of interfacial events difficult, necessitating interpretation from model interfacial systems or analysis post-fabrication. The review will focus on: i) the emulsification efficiency of proteins, cf. interfacial adsorption; ii) current excipients and techniques used to stabilise proteins, outlining work towards the oral delivery of insulin as a case study; iii) analytical techniques used to characterise encapsulated protein. There appears to be a recent trend towards the stabilization of proteins via direct complexation with polymers but, in contrast, emulsion techniques are emerging which err away from the use of stabilisers and/or excipients. A number of spectroscopic and spectrometric methods have found new application to the study of protein integrity in microspheres.

AB - Encapsulation of biomacromolecules in polyester micro- and nano-particles is now a routine procedure in many laboratories for achieving controlled and targeted delivery strategies. Objectives: Proteins are notoriously difficult to encapsulate without some degree of unfolding and loss of bioactivity, and this is despite around two decades of research. A case by case analysis appears necessary when determining which mechanism, generally unfolding at the emulsion interface or acidification of the particle interior, is most detrimental. The transient nature of the emulsion systems makes in situ, real-time analysis of interfacial events difficult, necessitating interpretation from model interfacial systems or analysis post-fabrication. The review will focus on: i) the emulsification efficiency of proteins, cf. interfacial adsorption; ii) current excipients and techniques used to stabilise proteins, outlining work towards the oral delivery of insulin as a case study; iii) analytical techniques used to characterise encapsulated protein. There appears to be a recent trend towards the stabilization of proteins via direct complexation with polymers but, in contrast, emulsion techniques are emerging which err away from the use of stabilisers and/or excipients. A number of spectroscopic and spectrometric methods have found new application to the study of protein integrity in microspheres.

KW - biomacromolecules

KW - polyester

KW - nano-particles

KW - drug delivery

UR - http://dx.doi.org/10.1517/17425240802680169

U2 - 10.1517/17425240802680169

DO - 10.1517/17425240802680169

M3 - Article

VL - 6

SP - 177

EP - 186

JO - Expert Opinion on Drug Delivery

T2 - Expert Opinion on Drug Delivery

JF - Expert Opinion on Drug Delivery

SN - 1742-5247

IS - 2

ER -