Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco

Heiko Ziebell, Alex M. Murphy, Simon C. Groen, Trisna Tungadi, Jack H. Westwood, Mathew G. Lewsey, Michael Moulin, Adam Kleczkowski, Alison G. Smith, Mark Stevens, Glen Powell, John P. Carr

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74 Citations (Scopus)
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Abstract

The cucumber mosaic virus (CMV) 2b protein not only inhibits anti-viral RNA silencing but also quenches transcriptional responses of plant genes to jasmonic acid, a key signalling molecule in defence against insects. This suggested that it might affect interactions between infected plants and aphids, insects that transmit CMV. We found that infection of tobacco with a 2b gene deletion mutant (CMVΔ2b) induced strong resistance to aphids (Myzus persicae) while CMV infection fostered aphid survival. Using electrical penetration graph methodology we found that higher proportions of aphids showed sustained phloem ingestion on CMV-infected plants than on CMVΔ2b-infected or mock-inoculated plants although this did not increase the rate of growth of individual aphids. This indicates that while CMV infection or certain viral gene products might elicit aphid resistance, the 2b protein normally counteracts this during a wild-type CMV infection. Our findings suggest that the 2b protein could indirectly affect aphid-mediated virus transmission.

Original languageEnglish
Article number187
Number of pages7
JournalScientific Reports
Volume1
DOIs
Publication statusPublished - 9 Dec 2011

Keywords

  • cucumber mosaic virus
  • anti-viral RNA silencing
  • virus protein
  • gene deletion
  • gene silencing
  • genetics
  • plant disease
  • tobacco
  • cyclopentane derivative
  • nicotine

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    Ziebell, H., Murphy, A. M., Groen, S. C., Tungadi, T., Westwood, J. H., Lewsey, M. G., ... Carr, J. P. (2011). Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco. Scientific Reports, 1, [187]. https://doi.org/10.1038/srep00187