Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco

Heiko Ziebell; Alex M. Murphy; Simon C. Groen; Trisna Tungadi; Jack H. Westwood; Mathew G. Lewsey; Michael Moulin; Adam Kleczkowski; Alison G. Smith; Mark Stevens; Glen Powell; John P. Carr

doi:10.1038/srep00187

Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco

Heiko Ziebell, Alex M. Murphy, Simon C. Groen, Trisna Tungadi, Jack H. Westwood, Mathew G. Lewsey, Michael Moulin, Adam Kleczkowski, Alison G. Smith, Mark Stevens, Glen Powell, John P. Carr^*

^*Corresponding author for this work

Mathematics And Statistics

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Abstract

The cucumber mosaic virus (CMV) 2b protein not only inhibits anti-viral RNA silencing but also quenches transcriptional responses of plant genes to jasmonic acid, a key signalling molecule in defence against insects. This suggested that it might affect interactions between infected plants and aphids, insects that transmit CMV. We found that infection of tobacco with a 2b gene deletion mutant (CMVΔ2b) induced strong resistance to aphids (Myzus persicae) while CMV infection fostered aphid survival. Using electrical penetration graph methodology we found that higher proportions of aphids showed sustained phloem ingestion on CMV-infected plants than on CMVΔ2b-infected or mock-inoculated plants although this did not increase the rate of growth of individual aphids. This indicates that while CMV infection or certain viral gene products might elicit aphid resistance, the 2b protein normally counteracts this during a wild-type CMV infection. Our findings suggest that the 2b protein could indirectly affect aphid-mediated virus transmission.

Original language	English
Article number	187
Number of pages	7
Journal	Scientific Reports
Volume	1
DOIs	https://doi.org/10.1038/srep00187
Publication status	Published - 9 Dec 2011

Funding

We thank Peter Palukaitis and John Walsh for valuable discussions. Research was funded by the Biotechnology and Biological Research Council (Grants BB/D008204/1 and BB/ D014376/1), The Leverhulme Trust (Grant F/09 741/F), the Cambridge University Newton Trust, and The Chadacre Trust. HZ was supported by a Walter Scott Fellowship from Trinity Hall, Cambridge, UK.

Keywords

cucumber mosaic virus
anti-viral RNA silencing
virus protein
gene deletion
gene silencing
genetics
plant disease
tobacco
cyclopentane derivative
nicotine

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10.1038/srep00187Licence: CC BY-NC-SA 3.0

Ziebell-etal-SR2011-Cucumber-mosaic-virus-2b-RNA-silencing-suppressor-modify-plant-aphid-interactionsFinal published version, 495 KBLicence: CC BY-NC-SA 3.0

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title = "Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco",

abstract = "The cucumber mosaic virus (CMV) 2b protein not only inhibits anti-viral RNA silencing but also quenches transcriptional responses of plant genes to jasmonic acid, a key signalling molecule in defence against insects. This suggested that it might affect interactions between infected plants and aphids, insects that transmit CMV. We found that infection of tobacco with a 2b gene deletion mutant (CMVΔ2b) induced strong resistance to aphids (Myzus persicae) while CMV infection fostered aphid survival. Using electrical penetration graph methodology we found that higher proportions of aphids showed sustained phloem ingestion on CMV-infected plants than on CMVΔ2b-infected or mock-inoculated plants although this did not increase the rate of growth of individual aphids. This indicates that while CMV infection or certain viral gene products might elicit aphid resistance, the 2b protein normally counteracts this during a wild-type CMV infection. Our findings suggest that the 2b protein could indirectly affect aphid-mediated virus transmission.",

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author = "Heiko Ziebell and Murphy, {Alex M.} and Groen, {Simon C.} and Trisna Tungadi and Westwood, {Jack H.} and Lewsey, {Mathew G.} and Michael Moulin and Adam Kleczkowski and Smith, {Alison G.} and Mark Stevens and Glen Powell and Carr, {John P.}",

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AU - Westwood, Jack H.

AU - Lewsey, Mathew G.

AU - Moulin, Michael

AU - Kleczkowski, Adam

AU - Smith, Alison G.

AU - Stevens, Mark

AU - Powell, Glen

AU - Carr, John P.

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KW - anti-viral RNA silencing

KW - virus protein

KW - gene deletion

KW - gene silencing

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KW - cyclopentane derivative

KW - nicotine

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Cucumber mosaic virus and its 2b RNA silencing suppressor modify plant-aphid interactions in tobacco

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