Cubic B-spline calibration for 3D super-resolution measurements using astigmatic imaging

Sven Proppert, Steve Wolter, Thorge Holm, Teresa Klein, Sebastian van de Linde, Markus Sauer

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In recent years three-dimensional (3D) super-resolution fluorescence imaging by single-molecule localization (localization microscopy) has gained considerable interest because of its simple implementation and high optical resolution. Astigmatic and biplane imaging are experimentally simple methods to engineer a 3D-specific point spread function (PSF), but existing evaluation methods have proven problematic in practical application. Here we introduce the use of cubic B-splines to model the relationship of axial position and PSF width in the above mentioned approaches and compare the performance with existing methods. We show that cubic B-splines are the first method that can combine precision, accuracy and simplicity.

LanguageEnglish
Pages10304-10316
Number of pages13
JournalOptics Express
Volume22
Issue number9
Early online date22 Apr 2014
DOIs
Publication statusPublished - 5 May 2014
Externally publishedYes

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splines
point spread functions
biplanes
engineers
microscopy
fluorescence
evaluation
molecules

Keywords

  • cubic B-splines
  • 3D super-resolution fluorescence imaging
  • localization microscopy
  • astigmatic imaging

Cite this

Proppert, Sven ; Wolter, Steve ; Holm, Thorge ; Klein, Teresa ; van de Linde, Sebastian ; Sauer, Markus. / Cubic B-spline calibration for 3D super-resolution measurements using astigmatic imaging. In: Optics Express. 2014 ; Vol. 22, No. 9. pp. 10304-10316.
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Cubic B-spline calibration for 3D super-resolution measurements using astigmatic imaging. / Proppert, Sven; Wolter, Steve; Holm, Thorge; Klein, Teresa; van de Linde, Sebastian; Sauer, Markus.

In: Optics Express, Vol. 22, No. 9, 05.05.2014, p. 10304-10316.

Research output: Contribution to journalArticle

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