Abstract
In recent years three-dimensional (3D) super-resolution fluorescence imaging by single-molecule localization (localization microscopy) has gained considerable interest because of its simple implementation and high optical resolution. Astigmatic and biplane imaging are experimentally simple methods to engineer a 3D-specific point spread function (PSF), but existing evaluation methods have proven problematic in practical application. Here we introduce the use of cubic B-splines to model the relationship of axial position and PSF width in the above mentioned approaches and compare the performance with existing methods. We show that cubic B-splines are the first method that can combine precision, accuracy and simplicity.
| Language | English |
|---|---|
| Pages | 10304-10316 |
| Number of pages | 13 |
| Journal | Optics Express |
| Volume | 22 |
| Issue number | 9 |
| Early online date | 22 Apr 2014 |
| DOIs | |
| Publication status | Published - 5 May 2014 |
| Externally published | Yes |
Fingerprint
Keywords
- cubic B-splines
- 3D super-resolution fluorescence imaging
- localization microscopy
- astigmatic imaging
Cite this
}
Cubic B-spline calibration for 3D super-resolution measurements using astigmatic imaging. / Proppert, Sven; Wolter, Steve; Holm, Thorge; Klein, Teresa; van de Linde, Sebastian; Sauer, Markus.
In: Optics Express, Vol. 22, No. 9, 05.05.2014, p. 10304-10316.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cubic B-spline calibration for 3D super-resolution measurements using astigmatic imaging
AU - Proppert, Sven
AU - Wolter, Steve
AU - Holm, Thorge
AU - Klein, Teresa
AU - van de Linde, Sebastian
AU - Sauer, Markus
PY - 2014/5/5
Y1 - 2014/5/5
N2 - In recent years three-dimensional (3D) super-resolution fluorescence imaging by single-molecule localization (localization microscopy) has gained considerable interest because of its simple implementation and high optical resolution. Astigmatic and biplane imaging are experimentally simple methods to engineer a 3D-specific point spread function (PSF), but existing evaluation methods have proven problematic in practical application. Here we introduce the use of cubic B-splines to model the relationship of axial position and PSF width in the above mentioned approaches and compare the performance with existing methods. We show that cubic B-splines are the first method that can combine precision, accuracy and simplicity.
AB - In recent years three-dimensional (3D) super-resolution fluorescence imaging by single-molecule localization (localization microscopy) has gained considerable interest because of its simple implementation and high optical resolution. Astigmatic and biplane imaging are experimentally simple methods to engineer a 3D-specific point spread function (PSF), but existing evaluation methods have proven problematic in practical application. Here we introduce the use of cubic B-splines to model the relationship of axial position and PSF width in the above mentioned approaches and compare the performance with existing methods. We show that cubic B-splines are the first method that can combine precision, accuracy and simplicity.
KW - cubic B-splines
KW - 3D super-resolution fluorescence imaging
KW - localization microscopy
KW - astigmatic imaging
UR - https://www.osapublishing.org/oe/home.cfm
U2 - 10.1364/OE.22.010304
DO - 10.1364/OE.22.010304
M3 - Article
VL - 22
SP - 10304
EP - 10316
JO - Optics Express
T2 - Optics Express
JF - Optics Express
SN - 1094-4087
IS - 9
ER -