TY - JOUR
T1 - Crystallization and preliminary crystallographic studies of antibacterial polypeptide LCI expressed in Escherichia coli
AU - Zhu, Jia-Peng
AU - Chen, Bing-Zhang
AU - Gong, Wei-Bin
AU - Liang, Yu-He
AU - Wang, Huan-Chen
AU - Xu, Qing
AU - Chen, Zhang-Liang
AU - Lu, Guang-Ying
PY - 2001/12/1
Y1 - 2001/12/1
N2 - LCI is a type of novel antibacterial polypeptide secreted by a Bacillus subtilis strain. It consists of 47 residues with a molecular weight of 5468 Da. Using bioengineering, LCI was expressed in Escherichia coli DH5α with recombinant plasmid pBVAB16. It was crystallized using PEG 4000 as a precipitant. The crystal belongs to space group P6222 or P6422, with unit-cell parameters a = b = 29.30, c = 187.09 Å, and diffracts to 2.44 Å. A set of diffraction data to 2.8 Å was collected.
AB - LCI is a type of novel antibacterial polypeptide secreted by a Bacillus subtilis strain. It consists of 47 residues with a molecular weight of 5468 Da. Using bioengineering, LCI was expressed in Escherichia coli DH5α with recombinant plasmid pBVAB16. It was crystallized using PEG 4000 as a precipitant. The crystal belongs to space group P6222 or P6422, with unit-cell parameters a = b = 29.30, c = 187.09 Å, and diffracts to 2.44 Å. A set of diffraction data to 2.8 Å was collected.
KW - antibacterial polypeptide
KW - Escherichia coli
KW - Bacillus subtilis strain
KW - LCI
UR - http://www.scopus.com/inward/record.url?scp=0035207619&partnerID=8YFLogxK
U2 - 10.1107/S0907444901017280
DO - 10.1107/S0907444901017280
M3 - Article
C2 - 11717521
AN - SCOPUS:0035207619
SN - 0907-4449
VL - D57
SP - 1931
EP - 1932
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 12
ER -