LCI is a type of novel antibacterial polypeptide secreted by a Bacillus subtilis strain. It consists of 47 residues with a molecular weight of 5468 Da. Using bioengineering, LCI was expressed in Escherichia coli DH5α with recombinant plasmid pBVAB16. It was crystallized using PEG 4000 as a precipitant. The crystal belongs to space group P6222 or P6422, with unit-cell parameters a = b = 29.30, c = 187.09 Å, and diffracts to 2.44 Å. A set of diffraction data to 2.8 Å was collected.
|Number of pages||2|
|Journal||Acta Crystallographica Section D: Biological Crystallography|
|Publication status||Published - 1 Dec 2001|
- antibacterial polypeptide
- Escherichia coli
- Bacillus subtilis strain