Crystallization and preliminary crystallographic studies of antibacterial polypeptide LCI expressed in Escherichia coli

Jia-Peng Zhu, Bing-Zhang Chen, Wei-Bin Gong, Yu-He Liang, Huan-Chen Wang, Qing Xu, Zhang-Liang Chen, Guang-Ying Lu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

LCI is a type of novel antibacterial polypeptide secreted by a Bacillus subtilis strain. It consists of 47 residues with a molecular weight of 5468 Da. Using bioengineering, LCI was expressed in Escherichia coli DH5α with recombinant plasmid pBVAB16. It was crystallized using PEG 4000 as a precipitant. The crystal belongs to space group P6222 or P6422, with unit-cell parameters a = b = 29.30, c = 187.09 Å, and diffracts to 2.44 Å. A set of diffraction data to 2.8 Å was collected.

LanguageEnglish
Pages1931-1932
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
VolumeD57
Issue number12
DOIs
Publication statusPublished - 1 Dec 2001

Fingerprint

Bioengineering
Crystallization
Bacillus subtilis
Plasmids
Molecular Weight
Escherichia coli
Peptides
antibacterial polypeptide LCI
Datasets

Keywords

  • antibacterial polypeptide
  • Escherichia coli
  • Bacillus subtilis strain
  • LCI

Cite this

Zhu, Jia-Peng ; Chen, Bing-Zhang ; Gong, Wei-Bin ; Liang, Yu-He ; Wang, Huan-Chen ; Xu, Qing ; Chen, Zhang-Liang ; Lu, Guang-Ying. / Crystallization and preliminary crystallographic studies of antibacterial polypeptide LCI expressed in Escherichia coli. In: Acta Crystallographica Section D: Biological Crystallography. 2001 ; Vol. D57, No. 12. pp. 1931-1932.
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Crystallization and preliminary crystallographic studies of antibacterial polypeptide LCI expressed in Escherichia coli. / Zhu, Jia-Peng; Chen, Bing-Zhang; Gong, Wei-Bin; Liang, Yu-He; Wang, Huan-Chen; Xu, Qing; Chen, Zhang-Liang; Lu, Guang-Ying.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. D57, No. 12, 01.12.2001, p. 1931-1932.

Research output: Contribution to journalArticle

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AU - Zhu, Jia-Peng

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AU - Xu, Qing

AU - Chen, Zhang-Liang

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