Cryopreserved hepatocyte monolayers

D. Stevenson, C. Morgan, E.I. Goldie, G. Connel, M.H. Grant

    Research output: Contribution to journalArticle

    Abstract

    Successful cryopreservation of hepatocytes would be useful to the pharmaceutical industry and for bioartificial liver support systems. Unfortunately, cryopreservation of hepatocytes in suspension results in low attachment efficiency in culture upon thawing. To circumvent this problem, we have frozen rat hepatocytes as monolayers on collagen substrates. Hepatocytes prepared from male Sprague Dawley rats by collagenase perfusion were cultured (3*106 cells/60mm Petri dish) on collagen films (30mg/cm2) in 2ml Chee's medium containing 5% v/v foetal calf serum (FCS). 24h cultures were frozen in Chee's medium with 10% Dimethyl Sulphoxide and between 0-90% FCS at -708C for 24h.
    Original languageEnglish
    Pages (from-to)94
    JournalBiochemical Society Transactions
    Volume30
    Publication statusPublished - 2002

    Keywords

    • toxicology
    • bioengineering
    • cryopreservation
    • hepatocytes
    • liver

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  • Cite this

    Stevenson, D., Morgan, C., Goldie, E. I., Connel, G., & Grant, M. H. (2002). Cryopreserved hepatocyte monolayers. Biochemical Society Transactions, 30, 94.