Creating permanent 3D arrangements of isolated cells using holographic optical tweezers

P. Jordan, J. Leach, M.J. Padgett, P. Blackburn, N. Isaacs, M. Goksor, D. Hanstorp, A.J. Wright, J.M. Girkin, J. Cooper

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Reports the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.
Original languageEnglish
Pages (from-to)1224-1228
Number of pages4
JournalLab on a Chip
Volume5
Issue number11
DOIs
Publication statusPublished - 2005

Keywords

  • 3-d cells
  • holographic
  • optical tweezers
  • optics
  • lasers

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