Creating permanent 3D arrangements of isolated cells using holographic optical tweezers

P. Jordan, J. Leach, M.J. Padgett, P. Blackburn, N. Isaacs, M. Goksor, D. Hanstorp, A.J. Wright, J.M. Girkin, J. Cooper

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Reports the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.
LanguageEnglish
Pages1224-1228
Number of pages4
JournalLab on a Chip
Volume5
Issue number11
DOIs
Publication statusPublished - 2005

Fingerprint

Optical Tweezers
Optical tweezers
Gelatin
Escherichia coli
Cell engineering
Cell Engineering
Nutrients
Laser beams
Lasers
Monomers
Tissue
Food
Growth

Keywords

  • 3-d cells
  • holographic
  • optical tweezers
  • optics
  • lasers

Cite this

Jordan, P., Leach, J., Padgett, M. J., Blackburn, P., Isaacs, N., Goksor, M., ... Cooper, J. (2005). Creating permanent 3D arrangements of isolated cells using holographic optical tweezers. Lab on a Chip, 5(11), 1224-1228. https://doi.org/10.1039/b509218c
Jordan, P. ; Leach, J. ; Padgett, M.J. ; Blackburn, P. ; Isaacs, N. ; Goksor, M. ; Hanstorp, D. ; Wright, A.J. ; Girkin, J.M. ; Cooper, J. / Creating permanent 3D arrangements of isolated cells using holographic optical tweezers. In: Lab on a Chip. 2005 ; Vol. 5, No. 11. pp. 1224-1228.
@article{bb36533c1a30420381965cd95ce7f35a,
title = "Creating permanent 3D arrangements of isolated cells using holographic optical tweezers",
abstract = "Reports the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.",
keywords = "3-d cells, holographic, optical tweezers, optics, lasers",
author = "P. Jordan and J. Leach and M.J. Padgett and P. Blackburn and N. Isaacs and M. Goksor and D. Hanstorp and A.J. Wright and J.M. Girkin and J. Cooper",
year = "2005",
doi = "10.1039/b509218c",
language = "English",
volume = "5",
pages = "1224--1228",
journal = "Lab on a Chip",
issn = "1473-0197",
number = "11",

}

Jordan, P, Leach, J, Padgett, MJ, Blackburn, P, Isaacs, N, Goksor, M, Hanstorp, D, Wright, AJ, Girkin, JM & Cooper, J 2005, 'Creating permanent 3D arrangements of isolated cells using holographic optical tweezers' Lab on a Chip, vol. 5, no. 11, pp. 1224-1228. https://doi.org/10.1039/b509218c

Creating permanent 3D arrangements of isolated cells using holographic optical tweezers. / Jordan, P.; Leach, J.; Padgett, M.J.; Blackburn, P.; Isaacs, N.; Goksor, M.; Hanstorp, D.; Wright, A.J.; Girkin, J.M.; Cooper, J.

In: Lab on a Chip, Vol. 5, No. 11, 2005, p. 1224-1228.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Creating permanent 3D arrangements of isolated cells using holographic optical tweezers

AU - Jordan, P.

AU - Leach, J.

AU - Padgett, M.J.

AU - Blackburn, P.

AU - Isaacs, N.

AU - Goksor, M.

AU - Hanstorp, D.

AU - Wright, A.J.

AU - Girkin, J.M.

AU - Cooper, J.

PY - 2005

Y1 - 2005

N2 - Reports the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.

AB - Reports the creation of permanent 3D configurations of cells, at predefined positions, within a gelatin matrix. The technique used holographic optical tweezers to manipulate individual E. coli within a solution comprising monomer precursors. The matrix was then set and after the laser beam was removed, we were able to demonstrate that the structures remained intact for many days. We were also able to demonstrate that, in the presence of appropriate nutrients, the E. coli survived within the gelatin matrix for several days. The technique could have a number of potential future applications, including the arrangement of a variety of different cell types in complex architectures, as motifs for promoting tissue differentiation and growth within the field of cell engineering.

KW - 3-d cells

KW - holographic

KW - optical tweezers

KW - optics

KW - lasers

UR - http://dx.doi.org/10.1039/b509218c

U2 - 10.1039/b509218c

DO - 10.1039/b509218c

M3 - Article

VL - 5

SP - 1224

EP - 1228

JO - Lab on a Chip

T2 - Lab on a Chip

JF - Lab on a Chip

SN - 1473-0197

IS - 11

ER -

Jordan P, Leach J, Padgett MJ, Blackburn P, Isaacs N, Goksor M et al. Creating permanent 3D arrangements of isolated cells using holographic optical tweezers. Lab on a Chip. 2005;5(11):1224-1228. https://doi.org/10.1039/b509218c