Creating advanced multifunctional biosensors with surface enzymatic transformations

H.J. Lee, A.W. Wark, R.M. Corn

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

This paper summarizes our recent work on the coupling of surface enzyme chemistry and bioaffinity interactions on biopolymer microarrays for the creation of multiplexed biosensors with enhanced selectivity and sensitivity. The
surface sensitive techniques of surface plasmon resonance imaging (SPRI) and surface plasmon fluorescence spectroscopy (SPFS) are used to detect the surface enzymatic transformations in real time. Three specific examples of novel coupled
surface bioaffinity/surface enzymatic processes are demonstrated: (i) a surface enzymatic amplification method utilizing the enzyme ribonuclease H (RNase H) in conjunction with RNA microarrays that permits the ultrasensitive direct
detection of genomic DNA at a concentration of 1 fM without labeling or PCR amplification, (ii) the use of RNADNA ligation chemistry to create renewable RNA microarrays from single stranded DNA microarrays, and (iii) the application of T7RNApolymerase for the on-chip replication ofRNAfrom double strandedDNAmicroarray elements. In addition, a simple yet powerful theoretical framework that includes the contributions of both enzyme adsorption
and surface enzyme kinetics is used to quantitate surface enzyme reactivity. This model is successfully applied to SPRI and SPFS measurements of surface hydrolysis reactions of RNase H and Exonuclease III (Exo III) on oligonucleotide
microarrays
LanguageEnglish
Pages5241-5250
Number of pages10
JournalLangmuir
Volume22
Issue number12
DOIs
Publication statusPublished - 6 Jun 2006

Fingerprint

bioinstrumentation
Biosensors
enzymes
Microarrays
Enzymes
Ribonuclease H
Fluorescence spectroscopy
Surface plasmon resonance
RNA
surface plasmon resonance
Amplification
DNA
deoxyribonucleic acid
chemistry
Imaging techniques
Enzyme kinetics
fluorescence
Biopolymers
Single-Stranded DNA
biopolymers

Keywords

  • surface enzymatic transformations
  • creating
  • advanced
  • multifunctional biosensors
  • enhanced fluorescence spectroscopy
  • resonance imaging measurements
  • label-free detections
  • plasmon resonance
  • dna microarrays
  • rna microarrays
  • real-time
  • ultrasensitive detection
  • point mutations
  • kinetics

Cite this

Lee, H.J. ; Wark, A.W. ; Corn, R.M. / Creating advanced multifunctional biosensors with surface enzymatic transformations. In: Langmuir. 2006 ; Vol. 22, No. 12. pp. 5241-5250.
@article{f6e345acc702422fa2fe2ab9e15483b5,
title = "Creating advanced multifunctional biosensors with surface enzymatic transformations",
abstract = "This paper summarizes our recent work on the coupling of surface enzyme chemistry and bioaffinity interactions on biopolymer microarrays for the creation of multiplexed biosensors with enhanced selectivity and sensitivity. The surface sensitive techniques of surface plasmon resonance imaging (SPRI) and surface plasmon fluorescence spectroscopy (SPFS) are used to detect the surface enzymatic transformations in real time. Three specific examples of novel coupledsurface bioaffinity/surface enzymatic processes are demonstrated: (i) a surface enzymatic amplification method utilizing the enzyme ribonuclease H (RNase H) in conjunction with RNA microarrays that permits the ultrasensitive directdetection of genomic DNA at a concentration of 1 fM without labeling or PCR amplification, (ii) the use of RNADNA ligation chemistry to create renewable RNA microarrays from single stranded DNA microarrays, and (iii) the application of T7RNApolymerase for the on-chip replication ofRNAfrom double strandedDNAmicroarray elements. In addition, a simple yet powerful theoretical framework that includes the contributions of both enzyme adsorptionand surface enzyme kinetics is used to quantitate surface enzyme reactivity. This model is successfully applied to SPRI and SPFS measurements of surface hydrolysis reactions of RNase H and Exonuclease III (Exo III) on oligonucleotidemicroarrays",
keywords = "surface enzymatic transformations, creating, advanced , multifunctional biosensors, enhanced fluorescence spectroscopy, resonance imaging measurements, label-free detections, plasmon resonance, dna microarrays, rna microarrays, real-time, ultrasensitive detection, point mutations, kinetics",
author = "H.J. Lee and A.W. Wark and R.M. Corn",
year = "2006",
month = "6",
day = "6",
doi = "10.1021/la060223o",
language = "English",
volume = "22",
pages = "5241--5250",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "12",

}

Creating advanced multifunctional biosensors with surface enzymatic transformations. / Lee, H.J.; Wark, A.W.; Corn, R.M.

In: Langmuir, Vol. 22, No. 12, 06.06.2006, p. 5241-5250.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Creating advanced multifunctional biosensors with surface enzymatic transformations

AU - Lee, H.J.

AU - Wark, A.W.

AU - Corn, R.M.

PY - 2006/6/6

Y1 - 2006/6/6

N2 - This paper summarizes our recent work on the coupling of surface enzyme chemistry and bioaffinity interactions on biopolymer microarrays for the creation of multiplexed biosensors with enhanced selectivity and sensitivity. The surface sensitive techniques of surface plasmon resonance imaging (SPRI) and surface plasmon fluorescence spectroscopy (SPFS) are used to detect the surface enzymatic transformations in real time. Three specific examples of novel coupledsurface bioaffinity/surface enzymatic processes are demonstrated: (i) a surface enzymatic amplification method utilizing the enzyme ribonuclease H (RNase H) in conjunction with RNA microarrays that permits the ultrasensitive directdetection of genomic DNA at a concentration of 1 fM without labeling or PCR amplification, (ii) the use of RNADNA ligation chemistry to create renewable RNA microarrays from single stranded DNA microarrays, and (iii) the application of T7RNApolymerase for the on-chip replication ofRNAfrom double strandedDNAmicroarray elements. In addition, a simple yet powerful theoretical framework that includes the contributions of both enzyme adsorptionand surface enzyme kinetics is used to quantitate surface enzyme reactivity. This model is successfully applied to SPRI and SPFS measurements of surface hydrolysis reactions of RNase H and Exonuclease III (Exo III) on oligonucleotidemicroarrays

AB - This paper summarizes our recent work on the coupling of surface enzyme chemistry and bioaffinity interactions on biopolymer microarrays for the creation of multiplexed biosensors with enhanced selectivity and sensitivity. The surface sensitive techniques of surface plasmon resonance imaging (SPRI) and surface plasmon fluorescence spectroscopy (SPFS) are used to detect the surface enzymatic transformations in real time. Three specific examples of novel coupledsurface bioaffinity/surface enzymatic processes are demonstrated: (i) a surface enzymatic amplification method utilizing the enzyme ribonuclease H (RNase H) in conjunction with RNA microarrays that permits the ultrasensitive directdetection of genomic DNA at a concentration of 1 fM without labeling or PCR amplification, (ii) the use of RNADNA ligation chemistry to create renewable RNA microarrays from single stranded DNA microarrays, and (iii) the application of T7RNApolymerase for the on-chip replication ofRNAfrom double strandedDNAmicroarray elements. In addition, a simple yet powerful theoretical framework that includes the contributions of both enzyme adsorptionand surface enzyme kinetics is used to quantitate surface enzyme reactivity. This model is successfully applied to SPRI and SPFS measurements of surface hydrolysis reactions of RNase H and Exonuclease III (Exo III) on oligonucleotidemicroarrays

KW - surface enzymatic transformations

KW - creating

KW - advanced

KW - multifunctional biosensors

KW - enhanced fluorescence spectroscopy

KW - resonance imaging measurements

KW - label-free detections

KW - plasmon resonance

KW - dna microarrays

KW - rna microarrays

KW - real-time

KW - ultrasensitive detection

KW - point mutations

KW - kinetics

UR - http://pubs.acs.org/doi/abs/10.1021/la060223o

U2 - 10.1021/la060223o

DO - 10.1021/la060223o

M3 - Article

VL - 22

SP - 5241

EP - 5250

JO - Langmuir

T2 - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 12

ER -