Cre-dependent optogenetic transgenic mice without early age-related hearing loss

Daniel Lyngholm, Shuzo Sakata

Research output: Contribution to journalArticle

Abstract

With the advent of recent genetic technologies for mice, it is now feasible to investigate the circuit mechanisms of brain functions in an unprecedented manner. Although transgenic mice are commonly used on C57BL/6J (C57) background, hearing research has typically relied on different genetic backgrounds, such as CBA/Ca or CBA due to the genetic defect of C57 mice for early age-related hearing loss. This limits the utilization of available genetic resources for hearing research. Here we report congenic (>F10) Cre-dependent channelrhodopsin2 (ChR2) mice on CBA/Ca background. By crossing this line with Cre-driver mice on C57 background, F1 hybrids restored the hearing deficit of C57 mice. We also found a linear relationship between aging and hearing loss, with progression rates varied depending on genetic backgrounds (3.39 dB/month for C57; 0.82 dB/month for F1 hybrid). We further demonstrate that this approach allows to express ChR2 in a specific type of inhibitory neurons in the auditory cortex and that they can be identified within a simultaneously recorded population of neurons in awake mice. Thus, our Cre-dependent optogenetic transgenic mice on CBA/Ca background are a valuable tool to investigate the circuit mechanisms of hearing across lifespan.
LanguageEnglish
Article number29
Number of pages8
JournalFrontiers in Aging Neuroscience
Volume11
DOIs
Publication statusPublished - 26 Feb 2019

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Optogenetics
Hearing Loss
Transgenic Mice
Hearing
Inbred C57BL Mouse
Neurons
Inbred CBA Mouse
Auditory Cortex
Research
Technology
Brain
Population

Keywords

  • optogenetics
  • neural circuit
  • aging
  • hearing
  • auditory cortex

Cite this

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Cre-dependent optogenetic transgenic mice without early age-related hearing loss. / Lyngholm, Daniel; Sakata, Shuzo.

In: Frontiers in Aging Neuroscience, Vol. 11, 29, 26.02.2019.

Research output: Contribution to journalArticle

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