Controlling stiffness in nanostructured hydrogels produced by enzymatic dephosphorylation

K. Thornton, A.M. Smith, C.L.R. Merry, R.V. Ulijn

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

In the present paper, we report on enzyme-initiated self-assembly of Fmoc (fluoren-9-ylmethoxycarbonyl)- tyrosine hydrogels by enzymatic dephosphorylation under physiological conditions and provide evidence for the ability to control the modulus. Upon enzyme action, a self-assembling network of interconnecting fibres is formed, observed by cryo-SEM (scanning electron microscopy) and TEM (transmission electron microscopy). The concentration of alkaline phosphatase added to the Fmoc-tyrosine phosphate ester precursor solution had a direct effect on the gelation time, mechanical properties and molecular arrangements as determined through oscillatory rheology, fluorescence spectroscopy and CD spectroscopy. This highly tuneable costeffective gel system may have applications in three-dimensional cell culture.
LanguageEnglish
Pages660-664
Number of pages4
JournalBiochemical Society Transactions
Volume37
Issue number4
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Hydrogels
Tyrosine
Stiffness
Scanning electron microscopy
Rheology
Fluorescence Spectrometry
Fluorescence spectroscopy
Gelation
Enzymes
Transmission Electron Microscopy
Cell culture
Electron Scanning Microscopy
Self assembly
Alkaline Phosphatase
Spectrum Analysis
Esters
Cell Culture Techniques
Phosphates
Spectroscopy
Transmission electron microscopy

Keywords

  • cell culture
  • hydrogel
  • nanostructure
  • phosphatase
  • stiffness

Cite this

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Controlling stiffness in nanostructured hydrogels produced by enzymatic dephosphorylation. / Thornton, K.; Smith, A.M.; Merry, C.L.R.; Ulijn, R.V.

In: Biochemical Society Transactions, Vol. 37, No. 4, 08.2009, p. 660-664.

Research output: Contribution to journalArticle

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