Controlling cell morphology on amino acid-modified cellulose

Deepak M. Kalaskar, Julie E. Gough, Rein V. Ulijn, William W. Sampson, David J. Scurr, Frank J. Rutten, Morgan R. Alexander, Catherine L. R. Merry, Stephen J. Eichhorn

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

This study shows that it is possible to affect the morphology and spreading of fibroblast cells on amino acid-modified cellulose-based fibrous networks. Hydrophilic (Gly, Ser), aliphatic (Ala, Val, Leu, Ile) and aromatic amino acids (Phe, Tyr, Trp), coupled to the cellulose via esterification, give rise to different cell morphologies. Modified cellulosic substrates are analysed using time of flight secondary ion mass spectrometry (TOF-SIMS), demonstrating that amino acids are coupled and uniformly distributed over the surface of the samples. Remarkably, it is shown that it is the aromatic amino acids, and in particular Trp, that give rise to significantly enhanced cell spreading. This enhanced effect is shown to be linked to an increase in the adsorption of fibronectin in the presence of aromatic bound amino acids.

Original languageEnglish
Pages (from-to)1059-1065
Number of pages7
JournalSoft Matter
Volume4
Issue number5
Early online date18 Mar 2008
DOIs
Publication statusPublished - 2008

Keywords

  • control
  • cell morphology
  • amino acid-modified cellulose

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    Kalaskar, D. M., Gough, J. E., Ulijn, R. V., Sampson, W. W., Scurr, D. J., Rutten, F. J., Alexander, M. R., Merry, C. L. R., & Eichhorn, S. J. (2008). Controlling cell morphology on amino acid-modified cellulose. Soft Matter, 4(5), 1059-1065. https://doi.org/10.1039/b719706n