Controlling cancer cell fate using localized biocatalytic self-assembly of an aromatic carbohydrate amphiphile

Ricardo A. Pires, Yousef M. Abul-Haija, Diana S. Costa, Ramon Novoa-Carballal, Rui L. Reis, Rein V. Ulijn, Iva Pashkuleva

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

We report on a simple carbohydrate amphiphile able to self-assemble into nanofibers upon enzymatic dephosphorylation. The self-assembly can be triggered by alkaline phosphatase (ALP) in solution or in situ by the ALP produced by osteosarcoma cell line, SaOs2. In the latter case, assembly and localized gelation occurs mainly on the cell surface. The gelation of the pericellular environment induces a reduction of the SaOs2 metabolic activity at an initial stage (≥7 h) that results in cell death at longer exposure periods (≥24 h). We show that this effect depends on the phosphatase concentration, and thus, it is cell-selective with prechondrocytes ATDC5 (that express ∼15-20 times lower ALP activity compared to SaOs2) not being affected at concentrations ≥1 mM. These results demonstrate that simple carbohydrate derivatives can be used in an antiosteosarcoma strategy with limited impact on the surrounding healthy cells/tissues.

LanguageEnglish
Pages576-579
Number of pages4
JournalJournal of the American Chemical Society
Volume137
Issue number2
Early online date25 Dec 2014
DOIs
Publication statusPublished - 25 Dec 2014

Fingerprint

Amphiphiles
Phosphatases
Carbohydrates
Self assembly
Alkaline Phosphatase
Cells
Gelation
Nanofibers
Neoplasms
Cell death
Osteosarcoma
Phosphoric Monoester Hydrolases
Cell Death
Tissue
Derivatives
Cell Line

Keywords

  • cancer cell fate
  • cells
  • enzymatic dephosphorylation
  • localized gelation
  • self-assembly
  • biocatalysis

Cite this

Pires, Ricardo A. ; Abul-Haija, Yousef M. ; Costa, Diana S. ; Novoa-Carballal, Ramon ; Reis, Rui L. ; Ulijn, Rein V. ; Pashkuleva, Iva. / Controlling cancer cell fate using localized biocatalytic self-assembly of an aromatic carbohydrate amphiphile. In: Journal of the American Chemical Society. 2014 ; Vol. 137, No. 2. pp. 576-579.
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Controlling cancer cell fate using localized biocatalytic self-assembly of an aromatic carbohydrate amphiphile. / Pires, Ricardo A.; Abul-Haija, Yousef M.; Costa, Diana S.; Novoa-Carballal, Ramon; Reis, Rui L.; Ulijn, Rein V.; Pashkuleva, Iva.

In: Journal of the American Chemical Society, Vol. 137, No. 2, 25.12.2014, p. 576-579.

Research output: Contribution to journalArticle

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