Controlled delivery of proteins into bilayer lipid membranes on chip

Michele Zagnoni, Mairi E. Sandison, Phedra Marius, Anthony G. Lee, Hywel Morgan

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.
LanguageEnglish
Pages1176 -1183
Number of pages8
JournalLab on a Chip
Volume7
Issue number9
DOIs
Publication statusPublished - 2007

Fingerprint

Lipid bilayers
Lipid Bilayers
Membrane Lipids
Electrophysiology
Artificial Membranes
Proteins
Membranes
Lab-on-a-chip
Preclinical Drug Evaluations
Microfluidics
Potassium Channels
Biosensing Techniques
Biosensors
Screening
Membrane Proteins
Fusion reactions
Technology
Equipment and Supplies
Potassium
Pharmaceutical Preparations

Keywords

  • proteins
  • bilayer lipid membranes
  • drug screening

Cite this

Zagnoni, Michele ; Sandison, Mairi E. ; Marius, Phedra ; Lee, Anthony G. ; Morgan, Hywel. / Controlled delivery of proteins into bilayer lipid membranes on chip. In: Lab on a Chip. 2007 ; Vol. 7, No. 9. pp. 1176 -1183 .
@article{6e708b58e24c4708b213404cd9c6ae05,
title = "Controlled delivery of proteins into bilayer lipid membranes on chip",
abstract = "The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.",
keywords = "proteins, bilayer lipid membranes, drug screening",
author = "Michele Zagnoni and Sandison, {Mairi E.} and Phedra Marius and Lee, {Anthony G.} and Hywel Morgan",
year = "2007",
doi = "10.1039/b703818f",
language = "English",
volume = "7",
pages = "1176 --1183",
journal = "Lab on a Chip",
issn = "1473-0197",
number = "9",

}

Controlled delivery of proteins into bilayer lipid membranes on chip. / Zagnoni, Michele; Sandison, Mairi E.; Marius, Phedra; Lee, Anthony G.; Morgan, Hywel.

In: Lab on a Chip, Vol. 7, No. 9, 2007, p. 1176 -1183 .

Research output: Contribution to journalArticle

TY - JOUR

T1 - Controlled delivery of proteins into bilayer lipid membranes on chip

AU - Zagnoni, Michele

AU - Sandison, Mairi E.

AU - Marius, Phedra

AU - Lee, Anthony G.

AU - Morgan, Hywel

PY - 2007

Y1 - 2007

N2 - The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.

AB - The study and the exploitation of membrane proteins for drug screening applications requires a controllable and reliable method for their delivery into an artificial suspended membrane platform based on lab-on-a-chip technology. In this work, a polymeric device for forming lipid bilayers suitable for electrophysiology studies and biosensor applications is presented. The chip supports a single bilayer and is configured for controlled protein delivery through on-chip microfluidics. In order to demonstrate the principle of protein delivery, the potassium channel KcsA was reconstituted into proteoliposomes, which were then fused with the suspended bilayer on-chip. Fusion of single proteoliposomes with the membrane was identified electrically. Single channel conductance measurements of KcsA in the on-chip bilayer were recorded and these were compared to previously published data obtained with a conventional planar bilayer system.

KW - proteins

KW - bilayer lipid membranes

KW - drug screening

U2 - 10.1039/b703818f

DO - 10.1039/b703818f

M3 - Article

VL - 7

SP - 1176

EP - 1183

JO - Lab on a Chip

T2 - Lab on a Chip

JF - Lab on a Chip

SN - 1473-0197

IS - 9

ER -