Constructing tissue-like complex structures using cell-laden DNA hydrogel bricks

Yijie Wang; Yu Shao; Xiaozhou Ma; Bini Zhou; Alan Faulkner-Jones; Wenmiao Shu; Dongsheng Liu

doi:10.1021/acsami.7b01604

Constructing tissue-like complex structures using cell-laden DNA hydrogel bricks

Yijie Wang, Yu Shao, Xiaozhou Ma, Bini Zhou, Alan Faulkner-Jones, Wenmiao Shu, Dongsheng Liu

Biomedical Engineering

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Abstract

Tissue engineering has long been a challenge because of the difficulty of addressing the requirements that such an engineered tissue must meet. In this paper, we developed a new "brick-to-wall" based on unique properties of DNA supramolecular hydrogels to fabricate three-dimensional (3D) tissuelike structures: different cell types are encapsulated in DNA hydrogel bricks which are then combined to build 3D structures. Signal responsiveness of cells through the DNA gels was evaluated and it was discovered that the gel permits cell migration in 3D. The results demonstrated that this technology is convenient, effective and reliable for cell manipulation, and we believe that it will benefit artificial tissue fabrication and future large-scale production.

Original language	English
Pages (from-to)	12311-12315
Number of pages	5
Journal	ACS Applied Materials and Interfaces
Volume	9
Issue number	14
Early online date	16 Mar 2017
DOIs	https://doi.org/10.1021/acsami.7b01604
Publication status	E-pub ahead of print - 16 Mar 2017

Keywords

DNA hydrogel
tissue engineering
cell arrangements
cell migration
self-healing
supramolecular

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10.1021/acsami.7b01604Licence: CC BY 4.0

Wang-etal-AMI2017-Constructing-tissue-like-complex-structures-using-cell-ladenFinal published version, 5.54 MBLicence: CC BY 4.0

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AU - Wang, Yijie

AU - Shao, Yu

AU - Ma, Xiaozhou

AU - Zhou, Bini

AU - Faulkner-Jones, Alan

AU - Shu, Wenmiao

AU - Liu, Dongsheng

PY - 2017/3/16

Y1 - 2017/3/16

N2 - Tissue engineering has long been a challenge because of the difficulty of addressing the requirements that such an engineered tissue must meet. In this paper, we developed a new "brick-to-wall" based on unique properties of DNA supramolecular hydrogels to fabricate three-dimensional (3D) tissuelike structures: different cell types are encapsulated in DNA hydrogel bricks which are then combined to build 3D structures. Signal responsiveness of cells through the DNA gels was evaluated and it was discovered that the gel permits cell migration in 3D. The results demonstrated that this technology is convenient, effective and reliable for cell manipulation, and we believe that it will benefit artificial tissue fabrication and future large-scale production.

AB - Tissue engineering has long been a challenge because of the difficulty of addressing the requirements that such an engineered tissue must meet. In this paper, we developed a new "brick-to-wall" based on unique properties of DNA supramolecular hydrogels to fabricate three-dimensional (3D) tissuelike structures: different cell types are encapsulated in DNA hydrogel bricks which are then combined to build 3D structures. Signal responsiveness of cells through the DNA gels was evaluated and it was discovered that the gel permits cell migration in 3D. The results demonstrated that this technology is convenient, effective and reliable for cell manipulation, and we believe that it will benefit artificial tissue fabrication and future large-scale production.

KW - DNA hydrogel

KW - tissue engineering

KW - cell arrangements

KW - cell migration

KW - self-healing

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JO - ACS Applied Materials and Interfaces

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Constructing tissue-like complex structures using cell-laden DNA hydrogel bricks

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