Confocal microscopy

Gail McConnell, William Amos, Tony Wilson

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

1150 Downloads (Pure)

Abstract

Chapter focusing on confocal microscopy. A confocal microscope is one in which the illumination is confined to a small volume in the specimen, the detection is confined to the same volume and the image is built up by scanning this volume over the specimen, either by moving the beam of light over the specimen or by displacing the specimen relative to a stationary beam. The chief
advantage of this type of microscope is that it gives a greatly enhanced discrimination of depth relative to conventional microscopes. Commercial systems appeared in the 1980s and, despite their high cost, the world market for them is probably between 500 and 1000 instruments per annum, mainly because of their use in biomedical research in conjunction with fluorescent labelling methods. There are many books and review articles on this
subject ( e.g. Pawley ( 2006) , Matsumoto( 2002), Wilson (1990) ). The purpose of this chapter is to provide an introduction to optical and engineering aspects that may be o f interest to biomedical users of confocal microscopy.
Original languageEnglish
Title of host publicationHandbook of Comprehensive Biophysics
Publication statusPublished - 2011

Keywords

  • confocal microscopy
  • confocal microscope
  • microscopy

Cite this

McConnell, G., Amos, W., & Wilson, T. (2011). Confocal microscopy. In Handbook of Comprehensive Biophysics