Confocal laser scanning microscopy (CLSM) study of hepatocytes cultured on collagen films and gels

M. Kataropoulou, E. Goldie, G. Connel, M.H. Grant

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Abstract

Summary of paper describing the process whereby primary hepatocyte cultures form an integral part of many hybrid artificial liver designs, and the extracellular matrix environment of the cultures is an important factor for optimal expression of hepatocyte-specific phenotype. This study investigates the effect of incorporating 20% chondroitin-6-sulphate (Ch6SO4), a glycosaminoglycan (GAG), into collagen films and gels, and crosslinking the films and the gels with 1,6-diaminohexane (DAH) on the viability of hepatocytes cultured for 48 hours.
Original languageEnglish
Pages (from-to)72-73
Number of pages2
JournalEuropean Cells and Materials
Volume4
Issue number2
Publication statusPublished - 2002

Keywords

  • lasers
  • confocal laser scanning
  • hepatocytes
  • collagen
  • bioengineering
  • toxicology

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