Confocal laser scanning fluorescence microscopy with a visible continuum source

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Abstract

Confocal laser scanning fluorescence microscopy is demonstrated using a photonic crystal fiber-based excitation source. A 38 cm-long section of photonic crystal fiber is pumped with femtosecond pulses from a Ti:sapphire laser, and the resultant visible continuum is selectively filtered to provide the peak excitation wavelengths required for a range of fluorescently labeled biological tissue.
Original languageEnglish
Pages (from-to)2844-2851
Number of pages7
JournalOptics Express
Volume12
Issue number13
Publication statusPublished - 2004

Keywords

  • optics
  • photonics
  • laser scanning
  • lasers
  • microscopy

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