Abstract
Confocal laser scanning fluorescence microscopy is demonstrated using a photonic crystal fiber-based excitation source. A 38 cm-long section of photonic crystal fiber is pumped with femtosecond pulses from a Ti:sapphire laser, and the resultant visible continuum is selectively filtered to provide the peak excitation wavelengths required for a range of fluorescently labeled biological tissue.
Original language | English |
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Pages (from-to) | 2844-2851 |
Number of pages | 7 |
Journal | Optics Express |
Volume | 12 |
Issue number | 13 |
Publication status | Published - 2004 |
Keywords
- optics
- photonics
- laser scanning
- lasers
- microscopy