Computer algebra approaches to enzyme kinetics

J. P.  Bennett; J. H. Davenport; M. C. Dewar; D. L.  Fisher; M. Grinfeld; H. M. Sauro

doi:10.1007/BFb0006927

Computer algebra approaches to enzyme kinetics

J. P. Bennett, J. H. Davenport, M. C. Dewar, D. L. Fisher, M. Grinfeld, H. M. Sauro

Research output: Contribution to journal › Article › peer-review

Abstract

The rate law governing the kinetics of a single enzyme mediated reaction may be derived relatively easily by hand given knowledge of the enzyme's mechanism. Such rate laws are typically non-linear in the concentrations of metabolites involved. When a number of enzymes interact the composite rate law for the complete system involves the simultaneous solution of the individual enzyme rate laws. We show how computer algebra can be used to solve this previously intractable problem, using the method of Gröbner Bases. We present an experimental example where kinetic parameters for individual enzymes are measured by making observations of a multi-enzyme system, and fitting these data to the rate law for the complete system.

Original language	English
Pages (from-to)	23-30
Number of pages	8
Journal	Lecture Notes in Control and Informaion Sciences
Volume	165
DOIs	https://doi.org/10.1007/BFb0006927
Publication status	Published - 1991

Keywords

kintetics
enzyme mechanisms

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10.1007/BFb0006927

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title = "Computer algebra approaches to enzyme kinetics",

abstract = "The rate law governing the kinetics of a single enzyme mediated reaction may be derived relatively easily by hand given knowledge of the enzyme's mechanism. Such rate laws are typically non-linear in the concentrations of metabolites involved. When a number of enzymes interact the composite rate law for the complete system involves the simultaneous solution of the individual enzyme rate laws. We show how computer algebra can be used to solve this previously intractable problem, using the method of Gr{\"o}bner Bases. We present an experimental example where kinetic parameters for individual enzymes are measured by making observations of a multi-enzyme system, and fitting these data to the rate law for the complete system.",

keywords = "kintetics, enzyme mechanisms",

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year = "1991",

doi = "10.1007/BFb0006927",

language = "English",

volume = "165",

pages = "23--30",

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TY - JOUR

T1 - Computer algebra approaches to enzyme kinetics

AU - Bennett, J. P.

AU - Davenport, J. H.

AU - Dewar, M. C.

AU - Fisher, D. L.

AU - Grinfeld, M.

AU - Sauro, H. M.

PY - 1991

Y1 - 1991

N2 - The rate law governing the kinetics of a single enzyme mediated reaction may be derived relatively easily by hand given knowledge of the enzyme's mechanism. Such rate laws are typically non-linear in the concentrations of metabolites involved. When a number of enzymes interact the composite rate law for the complete system involves the simultaneous solution of the individual enzyme rate laws. We show how computer algebra can be used to solve this previously intractable problem, using the method of Gröbner Bases. We present an experimental example where kinetic parameters for individual enzymes are measured by making observations of a multi-enzyme system, and fitting these data to the rate law for the complete system.

AB - The rate law governing the kinetics of a single enzyme mediated reaction may be derived relatively easily by hand given knowledge of the enzyme's mechanism. Such rate laws are typically non-linear in the concentrations of metabolites involved. When a number of enzymes interact the composite rate law for the complete system involves the simultaneous solution of the individual enzyme rate laws. We show how computer algebra can be used to solve this previously intractable problem, using the method of Gröbner Bases. We present an experimental example where kinetic parameters for individual enzymes are measured by making observations of a multi-enzyme system, and fitting these data to the rate law for the complete system.

KW - kintetics

KW - enzyme mechanisms

U2 - 10.1007/BFb0006927

DO - 10.1007/BFb0006927

M3 - Article

SN - 0170-8643

VL - 165

SP - 23

EP - 30

JO - Lecture Notes in Control and Informaion Sciences

JF - Lecture Notes in Control and Informaion Sciences

ER -

Computer algebra approaches to enzyme kinetics

Abstract

Keywords

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