Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study

Ticiano Gomes do Nascimento; Rodolfo Elleson dos Santos Arruda; Erika Tayse da Cruz Almeida; José Marcos dos Santos Oliveira; Irinaldo Diniz Basílio-Júnior; Isabel Cristina Celerino de Moraes Porto; Adilson Rodrigues Sabino; Josealdo Tonholo; Alexander Gray; RuAngelie Edrada Ebel; Carol Clements; Tong Zhang; David George Watson

doi:10.1038/s41598-019-54591-3

Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study

Ticiano Gomes do Nascimento^*, Rodolfo Elleson dos Santos Arruda, Erika Tayse da Cruz Almeida, José Marcos dos Santos Oliveira, Irinaldo Diniz Basílio-Júnior, Isabel Cristina Celerino de Moraes Porto, Adilson Rodrigues Sabino, Josealdo Tonholo, Alexander Gray, RuAngelie Edrada Ebel, Carol Clements, Tong Zhang, David George Watson

^*Corresponding author for this work

Strathclyde Institute Of Pharmacy And Biomedical Sciences

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Abstract

The standardization of apiceutical products like as propolis extracts has been widely debated worldwide and variations in the propolis chemical composition are still very relevant topics for use-standardized of different propolis-type as medication by much of the world’s population. The present manuscript discuss important issues related to the climate effect and variations in propolis metabolite-profiling changes, antioxidant capacity and variations of the antibacterial activity of the Brazilian red propolis metabolites using comprehensive multivariate correlations. It was observed the increasing of guttiferones concentrations during the intense drought period and drastic decreasing in rainy period. The climate variation induced the high concentration of flavonoids in rainy period with pronounced dropped in some rainy months. The Pearson´s analysis demonstrated correlation between IC₅₀ from DPPH and guttiferones and flavonoids concentrations. The PCA-X and Hotelling T2 test showed outliers during the months with lowest concentrations of formononetin and isoliquiritigenin was observed in antibacterial tests. The PLS-DA, OPLS-DA and VIP analysis demonstrate guttiferone E, guttiferone B, liquiritigenin, naringenin are considered important substances responsible by anti-staphylococcal activity in red propolis composition during the rainy season and drought period, but a synergistic effect with other flavonoids and isoflavonoids are not ruled out.

Original language	English
Article number	18293
Pages (from-to)	1-16
Number of pages	16
Journal	Scientific Reports
Volume	9
DOIs	https://doi.org/10.1038/s41598-019-54591-3
Publication status	Published - 4 Dec 2019

Funding

The authors would like to acknowledge SIPBS/Strathclyde University, CNPq (The Brazilian National Council for Scientific and Technological Development), CAPES (The Brazilian Coordination for the Personal Improvement of Superior Education) and FAPEAL (Foundation for Sponsoring Research in the State of Alagoas) for the scholarships of the Master’s course in Nutrition (PPGNUT) and Master Course in Pharmaceutical Sciences (PPGCF) and CNPq for financial support (Grant Number 446630/2014-4) according to the financial aid to the researchers 14/2014-Universal/MCT/CNPq and FAPEAL for financial support (Grant Number 600 30 000431/2017). The authors would also like to thank the Microbiological Quality Control Laboratory for Food analysis of the Nutrition College of the Federal University of Alagoas and to the Beekeeper: José Marinho de Lima (in memorian) for their support in collecting raw material and for the red propolis donations.

Keywords

antiparasitic agents
drug safety
metabolomics
natural products

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Cite this

do Nascimento, T. G., dos Santos Arruda, R. E., da Cruz Almeida, E. T., dos Santos Oliveira, J. M., Basílio-Júnior, I. D., Celerino de Moraes Porto, I. C., Rodrigues Sabino, A., Tonholo, J., Gray, A., Edrada Ebel, R., Clements, C., Zhang, T., & Watson, D. G. (2019). Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study. Scientific Reports, 9, 1-16. Article 18293. https://doi.org/10.1038/s41598-019-54591-3

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title = "Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study",

abstract = "The standardization of apiceutical products like as propolis extracts has been widely debated worldwide and variations in the propolis chemical composition are still very relevant topics for use-standardized of different propolis-type as medication by much of the world{\textquoteright}s population. The present manuscript discuss important issues related to the climate effect and variations in propolis metabolite-profiling changes, antioxidant capacity and variations of the antibacterial activity of the Brazilian red propolis metabolites using comprehensive multivariate correlations. It was observed the increasing of guttiferones concentrations during the intense drought period and drastic decreasing in rainy period. The climate variation induced the high concentration of flavonoids in rainy period with pronounced dropped in some rainy months. The Pearson´s analysis demonstrated correlation between IC50 from DPPH and guttiferones and flavonoids concentrations. The PCA-X and Hotelling T2 test showed outliers during the months with lowest concentrations of formononetin and isoliquiritigenin was observed in antibacterial tests. The PLS-DA, OPLS-DA and VIP analysis demonstrate guttiferone E, guttiferone B, liquiritigenin, naringenin are considered important substances responsible by anti-staphylococcal activity in red propolis composition during the rainy season and drought period, but a synergistic effect with other flavonoids and isoflavonoids are not ruled out.",

keywords = "antiparasitic agents, drug safety, metabolomics, natural products",

author = "{do Nascimento}, {Ticiano Gomes} and {dos Santos Arruda}, {Rodolfo Elleson} and {da Cruz Almeida}, {Erika Tayse} and {dos Santos Oliveira}, {Jos{\'e} Marcos} and Bas{\'i}lio-J{\'u}nior, {Irinaldo Diniz} and {Celerino de Moraes Porto}, {Isabel Cristina} and {Rodrigues Sabino}, Adilson and Josealdo Tonholo and Alexander Gray and {Edrada Ebel}, RuAngelie and Carol Clements and Tong Zhang and Watson, {David George}",

year = "2019",

month = dec,

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doi = "10.1038/s41598-019-54591-3",

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do Nascimento, TG, dos Santos Arruda, RE, da Cruz Almeida, ET, dos Santos Oliveira, JM, Basílio-Júnior, ID, Celerino de Moraes Porto, IC, Rodrigues Sabino, A, Tonholo, J, Gray, A, Edrada Ebel, R, Clements, C, Zhang, T & Watson, DG 2019, 'Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study', Scientific Reports, vol. 9, 18293, pp. 1-16. https://doi.org/10.1038/s41598-019-54591-3

Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study. / do Nascimento, Ticiano Gomes; dos Santos Arruda, Rodolfo Elleson; da Cruz Almeida, Erika Tayse et al.
In: Scientific Reports, Vol. 9, 18293, 04.12.2019, p. 1-16.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study

AU - do Nascimento, Ticiano Gomes

AU - dos Santos Arruda, Rodolfo Elleson

AU - da Cruz Almeida, Erika Tayse

AU - dos Santos Oliveira, José Marcos

AU - Basílio-Júnior, Irinaldo Diniz

AU - Celerino de Moraes Porto, Isabel Cristina

AU - Rodrigues Sabino, Adilson

AU - Tonholo, Josealdo

AU - Gray, Alexander

AU - Edrada Ebel, RuAngelie

AU - Clements, Carol

AU - Zhang, Tong

AU - Watson, David George

PY - 2019/12/4

Y1 - 2019/12/4

N2 - The standardization of apiceutical products like as propolis extracts has been widely debated worldwide and variations in the propolis chemical composition are still very relevant topics for use-standardized of different propolis-type as medication by much of the world’s population. The present manuscript discuss important issues related to the climate effect and variations in propolis metabolite-profiling changes, antioxidant capacity and variations of the antibacterial activity of the Brazilian red propolis metabolites using comprehensive multivariate correlations. It was observed the increasing of guttiferones concentrations during the intense drought period and drastic decreasing in rainy period. The climate variation induced the high concentration of flavonoids in rainy period with pronounced dropped in some rainy months. The Pearson´s analysis demonstrated correlation between IC50 from DPPH and guttiferones and flavonoids concentrations. The PCA-X and Hotelling T2 test showed outliers during the months with lowest concentrations of formononetin and isoliquiritigenin was observed in antibacterial tests. The PLS-DA, OPLS-DA and VIP analysis demonstrate guttiferone E, guttiferone B, liquiritigenin, naringenin are considered important substances responsible by anti-staphylococcal activity in red propolis composition during the rainy season and drought period, but a synergistic effect with other flavonoids and isoflavonoids are not ruled out.

AB - The standardization of apiceutical products like as propolis extracts has been widely debated worldwide and variations in the propolis chemical composition are still very relevant topics for use-standardized of different propolis-type as medication by much of the world’s population. The present manuscript discuss important issues related to the climate effect and variations in propolis metabolite-profiling changes, antioxidant capacity and variations of the antibacterial activity of the Brazilian red propolis metabolites using comprehensive multivariate correlations. It was observed the increasing of guttiferones concentrations during the intense drought period and drastic decreasing in rainy period. The climate variation induced the high concentration of flavonoids in rainy period with pronounced dropped in some rainy months. The Pearson´s analysis demonstrated correlation between IC50 from DPPH and guttiferones and flavonoids concentrations. The PCA-X and Hotelling T2 test showed outliers during the months with lowest concentrations of formononetin and isoliquiritigenin was observed in antibacterial tests. The PLS-DA, OPLS-DA and VIP analysis demonstrate guttiferone E, guttiferone B, liquiritigenin, naringenin are considered important substances responsible by anti-staphylococcal activity in red propolis composition during the rainy season and drought period, but a synergistic effect with other flavonoids and isoflavonoids are not ruled out.

KW - antiparasitic agents

KW - drug safety

KW - metabolomics

KW - natural products

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do Nascimento TG, dos Santos Arruda RE, da Cruz Almeida ET, dos Santos Oliveira JM, Basílio-Júnior ID, Celerino de Moraes Porto IC et al. Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study. Scientific Reports. 2019 Dec 4;9:1-16. 18293. doi: 10.1038/s41598-019-54591-3

Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study

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