Complete reversible refolding of a G-protein coupled receptor on a solid support

Natalie Di Bartolo, Emma L. R. Compton, Tony Warne, Patricia C. Edwards, Christopher G. Tate, Gebhard F. X. Schertler, Paula J. Booth

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    The factors defining the correct folding and stability of integral membrane proteins are poorly understood. Folding of only a few select membrane proteins has been scrutinised, leaving considerable deficiencies in knowledge for large protein families, such as G protein coupled receptors (GPCRs). Complete reversible folding, which is problematic for any membrane protein, has eluded this dominant receptor family. Moreover, attempts to recover receptors from denatured states are inefficient, yielding at best 40-70% functional protein. We present a method for the reversible unfolding of an archetypal family member, the β1-adrenergic receptor, and attain 100% recovery of the folded, functional state, in terms of ligand binding, compared to receptor which has not been subject to any unfolding and retains its original, folded structure. We exploit refolding on a solid support, which could avoid unwanted interactions and aggregation that occur in bulk solution. We determine the changes in structure and function upon unfolding and refolding. Additionally, we employ a method that is relatively new to membrane protein folding; pulse proteolysis. Complete refolding of β1-adrenergic receptor occurs in n-decyl-β-D-maltoside (DM) micelles from a urea-denatured state, as shown by regain of its original helical structure, ligand binding and protein fluorescence. The successful refolding strategy on a solid support offers a defined method for the controlled refolding and recovery of functional GPCRs and other membrane proteins that suffer from instability and irreversible denaturation once isolated from their native membranes.

    LanguageEnglish
    Article number0151582
    Number of pages16
    JournalPLOS One
    Volume11
    Issue number3
    DOIs
    Publication statusPublished - 16 Mar 2016

    Fingerprint

    G-Protein-Coupled Receptors
    membrane proteins
    Membrane Proteins
    adrenergic receptors
    Adrenergic Receptors
    receptors
    Proteolysis
    Ligands
    Protein folding
    Recovery
    Regain
    Denaturation
    protein folding
    proteins
    Protein Folding
    Micelles
    micelles
    denaturation
    proteolysis
    Urea

    Keywords

    • circular dichroism
    • electrophoresis, polyacrylamide gel
    • ligands
    • protein folding
    • protein unfolding
    • proteolysis
    • receptors, G-protein-coupled
    • spectrometry, fluorescence
    • spectrophotometry, ultraviolet
    • urea

    Cite this

    Di Bartolo, N., Compton, E. L. R., Warne, T., Edwards, P. C., Tate, C. G., Schertler, G. F. X., & Booth, P. J. (2016). Complete reversible refolding of a G-protein coupled receptor on a solid support. PLOS One, 11(3), [0151582]. https://doi.org/10.1371/journal.pone.0151582
    Di Bartolo, Natalie ; Compton, Emma L. R. ; Warne, Tony ; Edwards, Patricia C. ; Tate, Christopher G. ; Schertler, Gebhard F. X. ; Booth, Paula J. / Complete reversible refolding of a G-protein coupled receptor on a solid support. In: PLOS One. 2016 ; Vol. 11, No. 3.
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    Di Bartolo, N, Compton, ELR, Warne, T, Edwards, PC, Tate, CG, Schertler, GFX & Booth, PJ 2016, 'Complete reversible refolding of a G-protein coupled receptor on a solid support' PLOS One, vol. 11, no. 3, 0151582. https://doi.org/10.1371/journal.pone.0151582

    Complete reversible refolding of a G-protein coupled receptor on a solid support. / Di Bartolo, Natalie; Compton, Emma L. R.; Warne, Tony; Edwards, Patricia C.; Tate, Christopher G.; Schertler, Gebhard F. X.; Booth, Paula J.

    In: PLOS One, Vol. 11, No. 3, 0151582, 16.03.2016.

    Research output: Contribution to journalArticle

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    KW - spectrophotometry, ultraviolet

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    Di Bartolo N, Compton ELR, Warne T, Edwards PC, Tate CG, Schertler GFX et al. Complete reversible refolding of a G-protein coupled receptor on a solid support. PLOS One. 2016 Mar 16;11(3). 0151582. https://doi.org/10.1371/journal.pone.0151582