Comparison of presumptive blood test kits including Hexagon OBTI

S.S. Tobe, N.N. Daeid

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hochmeister et al. (1) performed validation tests for the Hexagon OBTI kit in 1999 and found a sensitivity of 1:100,000 when diluted in sterile water, a magnitude more sensitive than found in the current study under standard conditions. Hochmeister et al. (1) also conducted specificity, degradation, and contamination studies and determined that there was no effect on DNA recovery. This study was complimented by the work of Hermon et al. (2) who determined that sensitivity could be increased with a reduction of the buffer solution from 2 mL to 200 lL and also with an increase in incubation time. The maximum sensitivity that Hermon et al. (2) achieved was 1:1,000, even with an increase in incubation times, which they attribute to different volumes of blood used for the initial sampling. They also tested the specificity of the assay using different species of animals as well as other human body fluids.
LanguageEnglish
Pages239-239
Number of pages0
JournalJournal of Forensic Sciences
Volume54
Issue number1
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Hematologic Tests
Body Fluids
Blood Volume
Human Body
Buffers
Water
DNA

Keywords

  • Hexagon OBTI
  • blood test kits

Cite this

Tobe, S.S. ; Daeid, N.N. / Comparison of presumptive blood test kits including Hexagon OBTI. In: Journal of Forensic Sciences. 2009 ; Vol. 54, No. 1. pp. 239-239.
@article{4b10490bf7304d7583392ffef78cc320,
title = "Comparison of presumptive blood test kits including Hexagon OBTI",
abstract = "Hochmeister et al. (1) performed validation tests for the Hexagon OBTI kit in 1999 and found a sensitivity of 1:100,000 when diluted in sterile water, a magnitude more sensitive than found in the current study under standard conditions. Hochmeister et al. (1) also conducted specificity, degradation, and contamination studies and determined that there was no effect on DNA recovery. This study was complimented by the work of Hermon et al. (2) who determined that sensitivity could be increased with a reduction of the buffer solution from 2 mL to 200 lL and also with an increase in incubation time. The maximum sensitivity that Hermon et al. (2) achieved was 1:1,000, even with an increase in incubation times, which they attribute to different volumes of blood used for the initial sampling. They also tested the specificity of the assay using different species of animals as well as other human body fluids.",
keywords = "Hexagon OBTI, blood test kits",
author = "S.S. Tobe and N.N. Daeid",
year = "2009",
month = "1",
doi = "10.1111/j.1556-4029.2008.00941.x",
language = "English",
volume = "54",
pages = "239--239",
journal = "Journal of Forensic Sciences",
issn = "0022-1198",
number = "1",

}

Comparison of presumptive blood test kits including Hexagon OBTI. / Tobe, S.S.; Daeid, N.N.

In: Journal of Forensic Sciences, Vol. 54, No. 1, 01.2009, p. 239-239.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Comparison of presumptive blood test kits including Hexagon OBTI

AU - Tobe, S.S.

AU - Daeid, N.N.

PY - 2009/1

Y1 - 2009/1

N2 - Hochmeister et al. (1) performed validation tests for the Hexagon OBTI kit in 1999 and found a sensitivity of 1:100,000 when diluted in sterile water, a magnitude more sensitive than found in the current study under standard conditions. Hochmeister et al. (1) also conducted specificity, degradation, and contamination studies and determined that there was no effect on DNA recovery. This study was complimented by the work of Hermon et al. (2) who determined that sensitivity could be increased with a reduction of the buffer solution from 2 mL to 200 lL and also with an increase in incubation time. The maximum sensitivity that Hermon et al. (2) achieved was 1:1,000, even with an increase in incubation times, which they attribute to different volumes of blood used for the initial sampling. They also tested the specificity of the assay using different species of animals as well as other human body fluids.

AB - Hochmeister et al. (1) performed validation tests for the Hexagon OBTI kit in 1999 and found a sensitivity of 1:100,000 when diluted in sterile water, a magnitude more sensitive than found in the current study under standard conditions. Hochmeister et al. (1) also conducted specificity, degradation, and contamination studies and determined that there was no effect on DNA recovery. This study was complimented by the work of Hermon et al. (2) who determined that sensitivity could be increased with a reduction of the buffer solution from 2 mL to 200 lL and also with an increase in incubation time. The maximum sensitivity that Hermon et al. (2) achieved was 1:1,000, even with an increase in incubation times, which they attribute to different volumes of blood used for the initial sampling. They also tested the specificity of the assay using different species of animals as well as other human body fluids.

KW - Hexagon OBTI

KW - blood test kits

UR - http://dx.doi.org/10.1111/j.1556-4029.2008.00941.x

U2 - 10.1111/j.1556-4029.2008.00941.x

DO - 10.1111/j.1556-4029.2008.00941.x

M3 - Article

VL - 54

SP - 239

EP - 239

JO - Journal of Forensic Sciences

T2 - Journal of Forensic Sciences

JF - Journal of Forensic Sciences

SN - 0022-1198

IS - 1

ER -