Comparative study of the determination of bupivacaine in human plasma by gas chromatography mass spectrometry and high-performance liquid chromatography

A. Tahraoui, D.G. Watson, G.G. Skellern, S.A. Hudson, P. Petrie, K. Faccenda

Research output: Contribution to journalArticle

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Abstract

A comparison was made between high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) as methods for determining bupivacaine in human plasma. Both methods utilized pentycaine as an internal standard, were found to be linear in the range 5-320 ng and had acceptable precision and accuracy. The precision for the HPLC method was better than that for the GC-MS method. The limits of detection of the HPLC and GC-MS methods were ca. 1.0 and 0.1 ng, respectively. Good agreement between the HPLC and GC-MS methods was obtained for the analysis of samples taken from a patient receiving bupivacaine topically. For most purposes the HPLC method would be slightly better. However, for samples containing interfering peaks GC-MS provides a higher degree of resolution from such interferants.
Original languageEnglish
Pages (from-to)251-257
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume15
Issue number2
DOIs
Publication statusPublished - Nov 1996

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Plasma Gases
Bupivacaine
High performance liquid chromatography
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
High Pressure Liquid Chromatography
Plasma (human)
Limit of Detection

Keywords

  • high-performance liquid-chromatography
  • gas chromatography mass spectrometry
  • bupivacaine
  • bioanalysis

Cite this

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abstract = "A comparison was made between high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) as methods for determining bupivacaine in human plasma. Both methods utilized pentycaine as an internal standard, were found to be linear in the range 5-320 ng and had acceptable precision and accuracy. The precision for the HPLC method was better than that for the GC-MS method. The limits of detection of the HPLC and GC-MS methods were ca. 1.0 and 0.1 ng, respectively. Good agreement between the HPLC and GC-MS methods was obtained for the analysis of samples taken from a patient receiving bupivacaine topically. For most purposes the HPLC method would be slightly better. However, for samples containing interfering peaks GC-MS provides a higher degree of resolution from such interferants.",
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Comparative study of the determination of bupivacaine in human plasma by gas chromatography mass spectrometry and high-performance liquid chromatography. / Tahraoui, A.; Watson, D.G.; Skellern, G.G.; Hudson, S.A.; Petrie, P.; Faccenda, K.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 15, No. 2, 11.1996, p. 251-257.

Research output: Contribution to journalArticle

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AU - Tahraoui, A.

AU - Watson, D.G.

AU - Skellern, G.G.

AU - Hudson, S.A.

AU - Petrie, P.

AU - Faccenda, K.

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AB - A comparison was made between high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) as methods for determining bupivacaine in human plasma. Both methods utilized pentycaine as an internal standard, were found to be linear in the range 5-320 ng and had acceptable precision and accuracy. The precision for the HPLC method was better than that for the GC-MS method. The limits of detection of the HPLC and GC-MS methods were ca. 1.0 and 0.1 ng, respectively. Good agreement between the HPLC and GC-MS methods was obtained for the analysis of samples taken from a patient receiving bupivacaine topically. For most purposes the HPLC method would be slightly better. However, for samples containing interfering peaks GC-MS provides a higher degree of resolution from such interferants.

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