Comparative study of the determination of bupivacaine in human plasma by gas chromatography mass spectrometry and high-performance liquid chromatography

A. Tahraoui, D.G. Watson, G.G. Skellern, S.A. Hudson, P. Petrie, K. Faccenda

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20 Citations (Scopus)

Abstract

A comparison was made between high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) as methods for determining bupivacaine in human plasma. Both methods utilized pentycaine as an internal standard, were found to be linear in the range 5-320 ng and had acceptable precision and accuracy. The precision for the HPLC method was better than that for the GC-MS method. The limits of detection of the HPLC and GC-MS methods were ca. 1.0 and 0.1 ng, respectively. Good agreement between the HPLC and GC-MS methods was obtained for the analysis of samples taken from a patient receiving bupivacaine topically. For most purposes the HPLC method would be slightly better. However, for samples containing interfering peaks GC-MS provides a higher degree of resolution from such interferants.
Original languageEnglish
Pages (from-to)251-257
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume15
Issue number2
DOIs
Publication statusPublished - Nov 1996

Keywords

  • high-performance liquid-chromatography
  • gas chromatography mass spectrometry
  • bupivacaine
  • bioanalysis

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