Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 251-257 |
| Number of pages | 6 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 15 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Nov 1996 |
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Keywords
- high-performance liquid-chromatography
- gas chromatography mass spectrometry
- bupivacaine
- bioanalysis
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Comparative study of the determination of bupivacaine in human plasma by gas chromatography mass spectrometry and high-performance liquid chromatography. / Tahraoui, A.; Watson, D.G.; Skellern, G.G.; Hudson, S.A.; Petrie, P.; Faccenda, K.
In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 15, No. 2, 11.1996, p. 251-257.Research output: Contribution to journal › Article
TY - JOUR
T1 - Comparative study of the determination of bupivacaine in human plasma by gas chromatography mass spectrometry and high-performance liquid chromatography
AU - Tahraoui, A.
AU - Watson, D.G.
AU - Skellern, G.G.
AU - Hudson, S.A.
AU - Petrie, P.
AU - Faccenda, K.
PY - 1996/11
Y1 - 1996/11
N2 - A comparison was made between high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) as methods for determining bupivacaine in human plasma. Both methods utilized pentycaine as an internal standard, were found to be linear in the range 5-320 ng and had acceptable precision and accuracy. The precision for the HPLC method was better than that for the GC-MS method. The limits of detection of the HPLC and GC-MS methods were ca. 1.0 and 0.1 ng, respectively. Good agreement between the HPLC and GC-MS methods was obtained for the analysis of samples taken from a patient receiving bupivacaine topically. For most purposes the HPLC method would be slightly better. However, for samples containing interfering peaks GC-MS provides a higher degree of resolution from such interferants.
AB - A comparison was made between high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) as methods for determining bupivacaine in human plasma. Both methods utilized pentycaine as an internal standard, were found to be linear in the range 5-320 ng and had acceptable precision and accuracy. The precision for the HPLC method was better than that for the GC-MS method. The limits of detection of the HPLC and GC-MS methods were ca. 1.0 and 0.1 ng, respectively. Good agreement between the HPLC and GC-MS methods was obtained for the analysis of samples taken from a patient receiving bupivacaine topically. For most purposes the HPLC method would be slightly better. However, for samples containing interfering peaks GC-MS provides a higher degree of resolution from such interferants.
KW - high-performance liquid-chromatography
KW - gas chromatography mass spectrometry
KW - bupivacaine
KW - bioanalysis
UR - http://dx.doi.org/10.1016/0731-7085(96)01774-8
U2 - 10.1016/0731-7085(96)01774-8
DO - 10.1016/0731-7085(96)01774-8
M3 - Article
VL - 15
SP - 251
EP - 257
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
SN - 0731-7085
IS - 2
ER -