Comparative sensitivity of Trichophyton and Aspergillus conidia to inactivation by violet-blue light exposure

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Abstract

The objective of this paper was to investigate the use of 405 nm light for inhibiting the growth of selected species of dermatophytic and saprophytic fungi. The increasing incidence and resilience of dermatophytic fungal infections is a major issue, and alternative treatment methods are being sought. The sensitivity of the dermatophytic fungi Trichophyton rubrum and Trichophyton mentagrophytes to 405 nm violet-blue light exposure was investigated, and the results compared with those obtained with the saprophytic fungus Aspergillus niger. Microconidia of T. rubrum and T. mentagrophytes and conidia of A. niger were seeded onto Sabauroud dextrose agar plates and irradiated with 405 nm light from an indium-gallium-nitride 99-DIE light-emitting diode (LED) array and the extent of inhibition was measured. Germination of the microconidia of the Trichophyton species was completely inhibited using an irradiance of 35 mW/cm2 for 4 h (dose of 504 J/cm2). Results: A. niger conidia showed greater resistance, and colonial growth developed after light exposure. In liquid suspension tests, 405 nm light dose levels of 360, 720, and 1440 J/cm2 resulted in complete inactivation of T. rubrum microconidia, whereas A. niger showed greater resistance, and at the highest dose level applied (1440 J/cm2 ) although A niger hyphae were completely inactivated, only a 3-log10 reduction of a 5-log10 conidial suspension was achieved. The study results demonstrate the relatively high sensitivity of Trichophyton microconidia to 405 nm violet-blue light, and this is may be of potential interest regarding the control and treatment of dermatophyte infections.
LanguageEnglish
Pages36-41
Number of pages6
JournalPhotomedicine and Laser Surgery
Volume34
Issue number1
Early online date23 Nov 2015
DOIs
Publication statusPublished - 11 Jan 2016

Fingerprint

Viola
Trichophyton
Fungal Spores
Aspergillus
Aspergillus niger
Light
Fungi
Suspensions
Dextrose
Gallium nitride
Niger
Arthrodermataceae
Hyphae
Mycoses
Indium
Dosimetry
Light emitting diodes
Growth
Germination
Agar

Keywords

  • saprophytic fungi
  • dermatophytic fungi
  • violet-blue light
  • Trichophyton rubrum
  • Aspergillus Conidia
  • dermatophytes
  • Aspergillus niger
  • photodynamic inactivation
  • mycelia
  • spores

Cite this

@article{a2f0f1db6d8c47259c81c2fa6f9c9a6e,
title = "Comparative sensitivity of Trichophyton and Aspergillus conidia to inactivation by violet-blue light exposure",
abstract = "The objective of this paper was to investigate the use of 405 nm light for inhibiting the growth of selected species of dermatophytic and saprophytic fungi. The increasing incidence and resilience of dermatophytic fungal infections is a major issue, and alternative treatment methods are being sought. The sensitivity of the dermatophytic fungi Trichophyton rubrum and Trichophyton mentagrophytes to 405 nm violet-blue light exposure was investigated, and the results compared with those obtained with the saprophytic fungus Aspergillus niger. Microconidia of T. rubrum and T. mentagrophytes and conidia of A. niger were seeded onto Sabauroud dextrose agar plates and irradiated with 405 nm light from an indium-gallium-nitride 99-DIE light-emitting diode (LED) array and the extent of inhibition was measured. Germination of the microconidia of the Trichophyton species was completely inhibited using an irradiance of 35 mW/cm2 for 4 h (dose of 504 J/cm2). Results: A. niger conidia showed greater resistance, and colonial growth developed after light exposure. In liquid suspension tests, 405 nm light dose levels of 360, 720, and 1440 J/cm2 resulted in complete inactivation of T. rubrum microconidia, whereas A. niger showed greater resistance, and at the highest dose level applied (1440 J/cm2 ) although A niger hyphae were completely inactivated, only a 3-log10 reduction of a 5-log10 conidial suspension was achieved. The study results demonstrate the relatively high sensitivity of Trichophyton microconidia to 405 nm violet-blue light, and this is may be of potential interest regarding the control and treatment of dermatophyte infections.",
keywords = "saprophytic fungi, dermatophytic fungi, violet-blue light, Trichophyton rubrum, Aspergillus Conidia, dermatophytes, Aspergillus niger, photodynamic inactivation, mycelia, spores",
author = "Sian Moorhead and Michelle MacLean and MacGregor, {Scott J.} and Anderson, {John G.}",
year = "2016",
month = "1",
day = "11",
doi = "10.1089/pho.2015.3922",
language = "English",
volume = "34",
pages = "36--41",
journal = "Photomedicine and Laser Surgery",
issn = "1549-5418",
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TY - JOUR

T1 - Comparative sensitivity of Trichophyton and Aspergillus conidia to inactivation by violet-blue light exposure

AU - Moorhead, Sian

AU - MacLean, Michelle

AU - MacGregor, Scott J.

AU - Anderson, John G.

PY - 2016/1/11

Y1 - 2016/1/11

N2 - The objective of this paper was to investigate the use of 405 nm light for inhibiting the growth of selected species of dermatophytic and saprophytic fungi. The increasing incidence and resilience of dermatophytic fungal infections is a major issue, and alternative treatment methods are being sought. The sensitivity of the dermatophytic fungi Trichophyton rubrum and Trichophyton mentagrophytes to 405 nm violet-blue light exposure was investigated, and the results compared with those obtained with the saprophytic fungus Aspergillus niger. Microconidia of T. rubrum and T. mentagrophytes and conidia of A. niger were seeded onto Sabauroud dextrose agar plates and irradiated with 405 nm light from an indium-gallium-nitride 99-DIE light-emitting diode (LED) array and the extent of inhibition was measured. Germination of the microconidia of the Trichophyton species was completely inhibited using an irradiance of 35 mW/cm2 for 4 h (dose of 504 J/cm2). Results: A. niger conidia showed greater resistance, and colonial growth developed after light exposure. In liquid suspension tests, 405 nm light dose levels of 360, 720, and 1440 J/cm2 resulted in complete inactivation of T. rubrum microconidia, whereas A. niger showed greater resistance, and at the highest dose level applied (1440 J/cm2 ) although A niger hyphae were completely inactivated, only a 3-log10 reduction of a 5-log10 conidial suspension was achieved. The study results demonstrate the relatively high sensitivity of Trichophyton microconidia to 405 nm violet-blue light, and this is may be of potential interest regarding the control and treatment of dermatophyte infections.

AB - The objective of this paper was to investigate the use of 405 nm light for inhibiting the growth of selected species of dermatophytic and saprophytic fungi. The increasing incidence and resilience of dermatophytic fungal infections is a major issue, and alternative treatment methods are being sought. The sensitivity of the dermatophytic fungi Trichophyton rubrum and Trichophyton mentagrophytes to 405 nm violet-blue light exposure was investigated, and the results compared with those obtained with the saprophytic fungus Aspergillus niger. Microconidia of T. rubrum and T. mentagrophytes and conidia of A. niger were seeded onto Sabauroud dextrose agar plates and irradiated with 405 nm light from an indium-gallium-nitride 99-DIE light-emitting diode (LED) array and the extent of inhibition was measured. Germination of the microconidia of the Trichophyton species was completely inhibited using an irradiance of 35 mW/cm2 for 4 h (dose of 504 J/cm2). Results: A. niger conidia showed greater resistance, and colonial growth developed after light exposure. In liquid suspension tests, 405 nm light dose levels of 360, 720, and 1440 J/cm2 resulted in complete inactivation of T. rubrum microconidia, whereas A. niger showed greater resistance, and at the highest dose level applied (1440 J/cm2 ) although A niger hyphae were completely inactivated, only a 3-log10 reduction of a 5-log10 conidial suspension was achieved. The study results demonstrate the relatively high sensitivity of Trichophyton microconidia to 405 nm violet-blue light, and this is may be of potential interest regarding the control and treatment of dermatophyte infections.

KW - saprophytic fungi

KW - dermatophytic fungi

KW - violet-blue light

KW - Trichophyton rubrum

KW - Aspergillus Conidia

KW - dermatophytes

KW - Aspergillus niger

KW - photodynamic inactivation

KW - mycelia

KW - spores

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U2 - 10.1089/pho.2015.3922

DO - 10.1089/pho.2015.3922

M3 - Article

VL - 34

SP - 36

EP - 41

JO - Photomedicine and Laser Surgery

T2 - Photomedicine and Laser Surgery

JF - Photomedicine and Laser Surgery

SN - 1549-5418

IS - 1

ER -