Comparative proteomic analysis of a potentially probiotic Lactobacillus pentosus MP-10 for the identification of key proteins involved in antibiotic resistance and biocide tolerance

María del Carmen Casado Muñoz, Nabil Benomar, Saïd Ennahar, Peter Horvatovich, Leyre Lavilla Lerma, Charles W. Knapp, Antonio Gálvez, Hikmate Abriouel

Research output: Contribution to journalArticle

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Abstract

Probiotic bacterial cultures require resistance mechanisms to avoid stress-related responses under challenging environmental conditions; however, understanding these traits is required to discern their utility in fermentative food preparations, versus clinical and agricultural risk. Here, we compared the proteomic responses of Lactobacillus pentosus MP-10, a potentially probiotic lactic acid bacteria isolated from brines of naturally fermented Aloreña
green table olives, exposed to sub-lethal concentrations of antibiotics (amoxicillin, chloramphenicol and tetracycline) and biocides (benzalkonium chloride and triclosan). Several genes became differentially expressed depending on antimicrobial exposure, such as the up-regulation of protein synthesis, and the down-regulation of
carbohydrate metabolism and energy production. The antimicrobials appeared to have altered Lb. pentosus MP-10 physiology to achieve a gain of cellular energy for survival. For example, biocide-adapted Lb. pentosus MP-10 exhibited a down-regulated phosphocarrier protein HPr and an unexpressed oxidoreductase. However, protein synthesis was over-expressed in antibiotic- and biocide-adapted cells (ribosomal proteins and
glutamyl-tRNA synthetase), possibly to compensate for damaged proteins targeted by antimicrobials. Furthermore, stress proteins, such as NADH peroxidase (Npx) and a small heat shock protein, were only overexpressed in antibiotic-adapted Lb. pentosus MP-10. Results showed that adaptation to sub-lethal concentrations of antimicrobials could be a good way to achieve desirable robustness of the probiotic Lb. pentosus MP-10 to various environmental and gastrointestinal conditions (e.g., acid and bile stresses).
Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalInternational Journal of Food Microbiology
Volume222
Early online date22 Jan 2016
DOIs
Publication statusPublished - 2 Apr 2016

Fingerprint

Lactobacillus pentosus
taxonomic keys
Biocides
biocides
Disinfectants
Probiotics
Antibiotics
Microbial Drug Resistance
antibiotic resistance
Proteomics
proteomics
probiotics
anti-infective agents
NAD+ peroxidase
Anti-Bacterial Agents
Proteins
antibiotics
lethal genes
Triclosan
Small Heat-Shock Proteins

Keywords

  • comparative proteomics
  • Lactobacillus pentosus
  • probiotics
  • antibiotics
  • biocides

Cite this

del Carmen Casado Muñoz, María ; Benomar, Nabil ; Ennahar, Saïd ; Horvatovich, Peter ; Lavilla Lerma, Leyre ; Knapp, Charles W. ; Gálvez, Antonio ; Abriouel, Hikmate. / Comparative proteomic analysis of a potentially probiotic Lactobacillus pentosus MP-10 for the identification of key proteins involved in antibiotic resistance and biocide tolerance. In: International Journal of Food Microbiology. 2016 ; Vol. 222. pp. 8-15.
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Comparative proteomic analysis of a potentially probiotic Lactobacillus pentosus MP-10 for the identification of key proteins involved in antibiotic resistance and biocide tolerance. / del Carmen Casado Muñoz, María; Benomar, Nabil; Ennahar, Saïd; Horvatovich, Peter; Lavilla Lerma, Leyre; Knapp, Charles W.; Gálvez, Antonio; Abriouel, Hikmate.

In: International Journal of Food Microbiology, Vol. 222, 02.04.2016, p. 8-15.

Research output: Contribution to journalArticle

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T1 - Comparative proteomic analysis of a potentially probiotic Lactobacillus pentosus MP-10 for the identification of key proteins involved in antibiotic resistance and biocide tolerance

AU - del Carmen Casado Muñoz, María

AU - Benomar, Nabil

AU - Ennahar, Saïd

AU - Horvatovich, Peter

AU - Lavilla Lerma, Leyre

AU - Knapp, Charles W.

AU - Gálvez, Antonio

AU - Abriouel, Hikmate

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AB - Probiotic bacterial cultures require resistance mechanisms to avoid stress-related responses under challenging environmental conditions; however, understanding these traits is required to discern their utility in fermentative food preparations, versus clinical and agricultural risk. Here, we compared the proteomic responses of Lactobacillus pentosus MP-10, a potentially probiotic lactic acid bacteria isolated from brines of naturally fermented Aloreñagreen table olives, exposed to sub-lethal concentrations of antibiotics (amoxicillin, chloramphenicol and tetracycline) and biocides (benzalkonium chloride and triclosan). Several genes became differentially expressed depending on antimicrobial exposure, such as the up-regulation of protein synthesis, and the down-regulation ofcarbohydrate metabolism and energy production. The antimicrobials appeared to have altered Lb. pentosus MP-10 physiology to achieve a gain of cellular energy for survival. For example, biocide-adapted Lb. pentosus MP-10 exhibited a down-regulated phosphocarrier protein HPr and an unexpressed oxidoreductase. However, protein synthesis was over-expressed in antibiotic- and biocide-adapted cells (ribosomal proteins andglutamyl-tRNA synthetase), possibly to compensate for damaged proteins targeted by antimicrobials. Furthermore, stress proteins, such as NADH peroxidase (Npx) and a small heat shock protein, were only overexpressed in antibiotic-adapted Lb. pentosus MP-10. Results showed that adaptation to sub-lethal concentrations of antimicrobials could be a good way to achieve desirable robustness of the probiotic Lb. pentosus MP-10 to various environmental and gastrointestinal conditions (e.g., acid and bile stresses).

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