Comparative cytotoxicity of fumonisin B1 in two cell lines derived from normal human bronchial epithelial cells using four distinct bioassay techniques

C. W. Lewis, J. E. Smith, J. G. Anderson, R.I. Freshney

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Abstract

This study focuses on the cytotoxic effects of fumonisin B1 (FB1 on both immortalised and immortalised and subsequently transfected normal human bronchial epithelial (NHBE) cells of human origin using four bioassays. While the MTT, Neutral Red and hexosaminidase colorimetrie assays showed little difference between the toxic effects on the two related cell lines, the clonogenic assay, measuring cell survival and proliferation, indicated that FB1 had a more toxic effect on the nontransfected cells. This kind of in vitro approach using cells which retain many characteristics of normal cell growth and differentiation can go some way to developing evaluation models for food safety in the case of mycotoxin contamination without resorting totally to whole animal testing. Nevertheless, one or two cytotoxicity tests may be inadequate for a complete appraisal of toxic potential: rather, as wide a range of methodologies as feasible should be employed initially before meaningful conclusions may be drawn.
Original languageEnglish
Pages (from-to)81-90
Number of pages10
JournalMycotoxin Res.
Volume15
Issue number2
DOIs
Publication statusPublished - 1 Jun 1999

Keywords

  • cytotoxicity
  • human bronchial epithelial cells
  • bioassay techniques
  • fumonisin B1 (FB1)

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