Comparative chronic in vitro toxicity of hexavalent chromium to osteoblasts and monocytes

V.K. Raghunathan, J.N.A. Tettey, E.M. Ellis, M.H. Grant

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

There is increasing concern regarding the long-term biological effects of exposure to low concentrations of metal ions, particularly chromium, in patients following implantation of metal-on-metal hip prostheses. To investigate this, we have evaluated the chronic in vitro response to clinically relevant concentrations of chromium VI in osteoblasts and monocytes over a 4-week period in continuous culture. The cell viability, intracellular reduced glutathione content, glutathione reductase activity and expression, and expression of glutamate cysteine ligase were monitored in both cell types. Monocytes were more susceptible to the toxicity of the metal, and at the end of 4 weeks exposure to 0.5 M Cr VI, the protein content of cultures had declined to 23.4% ± 1.0% of control cultures. Both cell types demonstrated temporal increases in reduced glutathione levels, glutathione reductase activity, and glutamate cysteine ligase expression. Only osteoblasts showed a transcriptional increase in reductase expression. Data suggest that both cell types mount an adaptive response to chronic exposure to Cr VI, but this is more potent in osteoblasts, and results in the relative resilience of this cell type to the effects of Cr VI on cell viability.
LanguageEnglish
Pages543-550
Number of pages8
JournalJournal of Biomedical Materials Research Part A
Volume88A
Issue number2
DOIs
Publication statusPublished - Feb 2009

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Osteoblasts
Toxicity
Monocytes
Chromium
Metals
Glutamate-Cysteine Ligase
Glutathione Reductase
Glutathione
Cell Survival
Cells
Hip prostheses
Hip Prosthesis
Cell culture
Ion implantation
Metal ions
Oxidoreductases
Ions
Proteins
chromium hexavalent ion
In Vitro Techniques

Keywords

  • in vitro chromium toxicity
  • monocytes
  • osteoblasts
  • metal orthopedic implants
  • bioengineering

Cite this

Raghunathan, V.K. ; Tettey, J.N.A. ; Ellis, E.M. ; Grant, M.H. / Comparative chronic in vitro toxicity of hexavalent chromium to osteoblasts and monocytes. In: Journal of Biomedical Materials Research Part A. 2009 ; Vol. 88A, No. 2. pp. 543-550.
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Comparative chronic in vitro toxicity of hexavalent chromium to osteoblasts and monocytes. / Raghunathan, V.K.; Tettey, J.N.A.; Ellis, E.M.; Grant, M.H.

In: Journal of Biomedical Materials Research Part A, Vol. 88A, No. 2, 02.2009, p. 543-550.

Research output: Contribution to journalArticle

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