Combining functionalised nanoparticles and SERS for the detection of DNA relating to disease

D. Graham, R. Stevenson, D.G. Thompson, L. Barrett, C. Dalton, K. Faulds

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

DNA functionalised nanoparticle probes offer new opportunities in analyte detection. Ultrasensitive, molecularly specific targeting of analytes is possible through the use of metallic nanoparticles and their ability to generate a surface enhanced Raman scattering (SERS) response. This is leading to a new range of diagnostic clinical probes based on SERS detection. Our approaches have shown how such probes can detect specific DNA sequences by using a biomolecular recognition event to 'turn on' a SERS response through a controlled assembly process of the DNA functionalised nanoparticles. Further, we have prepared DNA aptamer functionalised SERS probes and demonstrated how introduction of a protein target can change the aggregation state of the nanoparticles in a dose-dependant manner. These approaches are being used as methods to detect biomolecules that indicate a specific disease being present with a view to improving disease management.
LanguageEnglish
Pages291-299
Number of pages9
JournalFaraday Discussions
Volume149
DOIs
Publication statusPublished - 2011

Fingerprint

Raman scattering
deoxyribonucleic acid
Raman spectra
Nanoparticles
nanoparticles
probes
DNA
Nucleotide Aptamers
DNA sequences
Biomolecules
Agglomeration
assembly
proteins
dosage
Proteins

Keywords

  • DNA
  • functionalised nanoparticle probes
  • analyte detection
  • raman scattering
  • SERS
  • biomolecular recognition
  • TIC - Bionanotechnology

Cite this

Graham, D. ; Stevenson, R. ; Thompson, D.G. ; Barrett, L. ; Dalton, C. ; Faulds, K. / Combining functionalised nanoparticles and SERS for the detection of DNA relating to disease. In: Faraday Discussions. 2011 ; Vol. 149. pp. 291-299.
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Combining functionalised nanoparticles and SERS for the detection of DNA relating to disease. / Graham, D.; Stevenson, R.; Thompson, D.G.; Barrett, L.; Dalton, C.; Faulds, K.

In: Faraday Discussions, Vol. 149, 2011, p. 291-299.

Research output: Contribution to journalArticle

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