Combined effects of ischemic preconditioning and nitrate supplementation on submaximal cycling exercise and time-trial performance

Luke C McIlvenna, Chris Monaghan, Bernadette O Fernandez, Martin Feelisch, David J. Muggeridge, Chris Easton

Research output: Contribution to conferenceAbstract

Abstract

Ischemic preconditioning (IPC) and dietary nitrate supplementation (DN) have both been shown to modulate nitric oxide (NO) availability. Despite the possibility of a synergistic response, the combined effects of IPC and DN during exercise have yet to be explored.PURPOSE: To determine the effects of IPC alone and in combination with DN on the physiological responses to submaximal cycling and time trial (TT) performance.METHODS: Following an initial maximal exercise test, nine competitive male cyclists (34 ± 6 Yr, V[Combining Dot Above]O2peak: 55 ± 4 mL/kg/min) completed a baseline trial (BASE), and two experimental trials in a double-blind randomized cross-over design. Exercise trials comprised 6 min of cycling at 80% of the ventilatory threshold, followed by a 16.1 km TT. In the experimental trials participants received either 500 mg of DN (chard gel) or a NO3--depleted placebo (PLA) 90 min before completing three cycles of bilateral lower limb IPC at 180 mmHg. Venous blood samples were collected pre- and post-IPC to determine changes in plasma nitrite [NO2-]. V[Combining Dot Above]O2 and HR were continuously monitored during submaximal exercise.RESULTS: Full arterial occlusion was confirmed via coloured Doppler in all trials. Prior to IPC, plasma [NO2-] was higher in DN (774 ± 179 nM, P=0.047) than BASE (576 ± 170 nM) and PLA (544 ± 126 nM, P=0.752). Following IPC plasma [NO2-] increased in PLA (?104 ± 149 nM, d=0.70) and DN (?42 ± 90 nM, d=0.47), but not significantly (both P>0.2). In the DN trial, resting V[Combining Dot Above]O2 was significantly lower compared to BASE (314 ± 69 vs. 367 ± 30 mL/min, P=0.02) and tended to be lower during exercise (P=0.066). Resting V[Combining Dot Above]O2 was also lower in PLA than BASE (323 ± 62 mL/min, P=0.01) and during exercise (2783 ± 262 vs. 3013 ± 342 mL/min, P=0.04). HR was not significantly different in submaximal exercise (P=0.842). Completion time of TT was not different between conditions (BASE: 1336 ± 73 s, PLA: 1344 ± 88 s, DN: 1344 ± 76 s, P=0.69). Compared to BASE (171 ± 4 bpm), HR was lower following DN (166 ± 4 bpm, P=0.02) but was not different in PLA (169 ± 4 bpm, P=0.60).CONCLUSION: In the present study, IPC with or without DN, altered a number of physiological responses during rest and submaximal exercise, potentially mediated by an increase in plasma [NO2-]. Despite this, there was no evidence for an additive effect and neither intervention altered TT performance.
Original languageEnglish
Number of pages1
Publication statusPublished - 1 Jun 2016
EventAmerican College of Sports Medicine Annual Meeting - Boston, MA, United States
Duration: 31 May 20164 Jun 2016
Conference number: 63rd

Conference

ConferenceAmerican College of Sports Medicine Annual Meeting
Abbreviated titleACSM16
CountryUnited States
CityBoston, MA
Period31/05/164/06/16

Fingerprint

Ischemic Preconditioning
Dietary Supplements
Nitrates
Placebos
Beta vulgaris
Nitrites
Exercise Test
Cross-Over Studies
Lower Extremity
Nitric Oxide
Gels

Keywords

  • nitrate supplementation
  • ischemic preconditioning
  • cycling

Cite this

McIlvenna, L. C., Monaghan, C., Fernandez, B. O., Feelisch, M., Muggeridge, D. J., & Easton, C. (2016). Combined effects of ischemic preconditioning and nitrate supplementation on submaximal cycling exercise and time-trial performance. Abstract from American College of Sports Medicine Annual Meeting, Boston, MA, United States.
McIlvenna, Luke C ; Monaghan, Chris ; Fernandez, Bernadette O ; Feelisch, Martin ; Muggeridge, David J. ; Easton, Chris. / Combined effects of ischemic preconditioning and nitrate supplementation on submaximal cycling exercise and time-trial performance. Abstract from American College of Sports Medicine Annual Meeting, Boston, MA, United States.1 p.
@conference{4f77a77c68e04f14832246fb1e56047f,
title = "Combined effects of ischemic preconditioning and nitrate supplementation on submaximal cycling exercise and time-trial performance",
abstract = "Ischemic preconditioning (IPC) and dietary nitrate supplementation (DN) have both been shown to modulate nitric oxide (NO) availability. Despite the possibility of a synergistic response, the combined effects of IPC and DN during exercise have yet to be explored.PURPOSE: To determine the effects of IPC alone and in combination with DN on the physiological responses to submaximal cycling and time trial (TT) performance.METHODS: Following an initial maximal exercise test, nine competitive male cyclists (34 ± 6 Yr, V[Combining Dot Above]O2peak: 55 ± 4 mL/kg/min) completed a baseline trial (BASE), and two experimental trials in a double-blind randomized cross-over design. Exercise trials comprised 6 min of cycling at 80{\%} of the ventilatory threshold, followed by a 16.1 km TT. In the experimental trials participants received either 500 mg of DN (chard gel) or a NO3--depleted placebo (PLA) 90 min before completing three cycles of bilateral lower limb IPC at 180 mmHg. Venous blood samples were collected pre- and post-IPC to determine changes in plasma nitrite [NO2-]. V[Combining Dot Above]O2 and HR were continuously monitored during submaximal exercise.RESULTS: Full arterial occlusion was confirmed via coloured Doppler in all trials. Prior to IPC, plasma [NO2-] was higher in DN (774 ± 179 nM, P=0.047) than BASE (576 ± 170 nM) and PLA (544 ± 126 nM, P=0.752). Following IPC plasma [NO2-] increased in PLA (?104 ± 149 nM, d=0.70) and DN (?42 ± 90 nM, d=0.47), but not significantly (both P>0.2). In the DN trial, resting V[Combining Dot Above]O2 was significantly lower compared to BASE (314 ± 69 vs. 367 ± 30 mL/min, P=0.02) and tended to be lower during exercise (P=0.066). Resting V[Combining Dot Above]O2 was also lower in PLA than BASE (323 ± 62 mL/min, P=0.01) and during exercise (2783 ± 262 vs. 3013 ± 342 mL/min, P=0.04). HR was not significantly different in submaximal exercise (P=0.842). Completion time of TT was not different between conditions (BASE: 1336 ± 73 s, PLA: 1344 ± 88 s, DN: 1344 ± 76 s, P=0.69). Compared to BASE (171 ± 4 bpm), HR was lower following DN (166 ± 4 bpm, P=0.02) but was not different in PLA (169 ± 4 bpm, P=0.60).CONCLUSION: In the present study, IPC with or without DN, altered a number of physiological responses during rest and submaximal exercise, potentially mediated by an increase in plasma [NO2-]. Despite this, there was no evidence for an additive effect and neither intervention altered TT performance.",
keywords = "nitrate supplementation, ischemic preconditioning, cycling",
author = "McIlvenna, {Luke C} and Chris Monaghan and Fernandez, {Bernadette O} and Martin Feelisch and Muggeridge, {David J.} and Chris Easton",
year = "2016",
month = "6",
day = "1",
language = "English",
note = "American College of Sports Medicine Annual Meeting, ACSM16 ; Conference date: 31-05-2016 Through 04-06-2016",

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McIlvenna, LC, Monaghan, C, Fernandez, BO, Feelisch, M, Muggeridge, DJ & Easton, C 2016, 'Combined effects of ischemic preconditioning and nitrate supplementation on submaximal cycling exercise and time-trial performance' American College of Sports Medicine Annual Meeting, Boston, MA, United States, 31/05/16 - 4/06/16, .

Combined effects of ischemic preconditioning and nitrate supplementation on submaximal cycling exercise and time-trial performance. / McIlvenna, Luke C; Monaghan, Chris; Fernandez, Bernadette O; Feelisch, Martin; Muggeridge, David J.; Easton, Chris.

2016. Abstract from American College of Sports Medicine Annual Meeting, Boston, MA, United States.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Combined effects of ischemic preconditioning and nitrate supplementation on submaximal cycling exercise and time-trial performance

AU - McIlvenna, Luke C

AU - Monaghan, Chris

AU - Fernandez, Bernadette O

AU - Feelisch, Martin

AU - Muggeridge, David J.

AU - Easton, Chris

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Ischemic preconditioning (IPC) and dietary nitrate supplementation (DN) have both been shown to modulate nitric oxide (NO) availability. Despite the possibility of a synergistic response, the combined effects of IPC and DN during exercise have yet to be explored.PURPOSE: To determine the effects of IPC alone and in combination with DN on the physiological responses to submaximal cycling and time trial (TT) performance.METHODS: Following an initial maximal exercise test, nine competitive male cyclists (34 ± 6 Yr, V[Combining Dot Above]O2peak: 55 ± 4 mL/kg/min) completed a baseline trial (BASE), and two experimental trials in a double-blind randomized cross-over design. Exercise trials comprised 6 min of cycling at 80% of the ventilatory threshold, followed by a 16.1 km TT. In the experimental trials participants received either 500 mg of DN (chard gel) or a NO3--depleted placebo (PLA) 90 min before completing three cycles of bilateral lower limb IPC at 180 mmHg. Venous blood samples were collected pre- and post-IPC to determine changes in plasma nitrite [NO2-]. V[Combining Dot Above]O2 and HR were continuously monitored during submaximal exercise.RESULTS: Full arterial occlusion was confirmed via coloured Doppler in all trials. Prior to IPC, plasma [NO2-] was higher in DN (774 ± 179 nM, P=0.047) than BASE (576 ± 170 nM) and PLA (544 ± 126 nM, P=0.752). Following IPC plasma [NO2-] increased in PLA (?104 ± 149 nM, d=0.70) and DN (?42 ± 90 nM, d=0.47), but not significantly (both P>0.2). In the DN trial, resting V[Combining Dot Above]O2 was significantly lower compared to BASE (314 ± 69 vs. 367 ± 30 mL/min, P=0.02) and tended to be lower during exercise (P=0.066). Resting V[Combining Dot Above]O2 was also lower in PLA than BASE (323 ± 62 mL/min, P=0.01) and during exercise (2783 ± 262 vs. 3013 ± 342 mL/min, P=0.04). HR was not significantly different in submaximal exercise (P=0.842). Completion time of TT was not different between conditions (BASE: 1336 ± 73 s, PLA: 1344 ± 88 s, DN: 1344 ± 76 s, P=0.69). Compared to BASE (171 ± 4 bpm), HR was lower following DN (166 ± 4 bpm, P=0.02) but was not different in PLA (169 ± 4 bpm, P=0.60).CONCLUSION: In the present study, IPC with or without DN, altered a number of physiological responses during rest and submaximal exercise, potentially mediated by an increase in plasma [NO2-]. Despite this, there was no evidence for an additive effect and neither intervention altered TT performance.

AB - Ischemic preconditioning (IPC) and dietary nitrate supplementation (DN) have both been shown to modulate nitric oxide (NO) availability. Despite the possibility of a synergistic response, the combined effects of IPC and DN during exercise have yet to be explored.PURPOSE: To determine the effects of IPC alone and in combination with DN on the physiological responses to submaximal cycling and time trial (TT) performance.METHODS: Following an initial maximal exercise test, nine competitive male cyclists (34 ± 6 Yr, V[Combining Dot Above]O2peak: 55 ± 4 mL/kg/min) completed a baseline trial (BASE), and two experimental trials in a double-blind randomized cross-over design. Exercise trials comprised 6 min of cycling at 80% of the ventilatory threshold, followed by a 16.1 km TT. In the experimental trials participants received either 500 mg of DN (chard gel) or a NO3--depleted placebo (PLA) 90 min before completing three cycles of bilateral lower limb IPC at 180 mmHg. Venous blood samples were collected pre- and post-IPC to determine changes in plasma nitrite [NO2-]. V[Combining Dot Above]O2 and HR were continuously monitored during submaximal exercise.RESULTS: Full arterial occlusion was confirmed via coloured Doppler in all trials. Prior to IPC, plasma [NO2-] was higher in DN (774 ± 179 nM, P=0.047) than BASE (576 ± 170 nM) and PLA (544 ± 126 nM, P=0.752). Following IPC plasma [NO2-] increased in PLA (?104 ± 149 nM, d=0.70) and DN (?42 ± 90 nM, d=0.47), but not significantly (both P>0.2). In the DN trial, resting V[Combining Dot Above]O2 was significantly lower compared to BASE (314 ± 69 vs. 367 ± 30 mL/min, P=0.02) and tended to be lower during exercise (P=0.066). Resting V[Combining Dot Above]O2 was also lower in PLA than BASE (323 ± 62 mL/min, P=0.01) and during exercise (2783 ± 262 vs. 3013 ± 342 mL/min, P=0.04). HR was not significantly different in submaximal exercise (P=0.842). Completion time of TT was not different between conditions (BASE: 1336 ± 73 s, PLA: 1344 ± 88 s, DN: 1344 ± 76 s, P=0.69). Compared to BASE (171 ± 4 bpm), HR was lower following DN (166 ± 4 bpm, P=0.02) but was not different in PLA (169 ± 4 bpm, P=0.60).CONCLUSION: In the present study, IPC with or without DN, altered a number of physiological responses during rest and submaximal exercise, potentially mediated by an increase in plasma [NO2-]. Despite this, there was no evidence for an additive effect and neither intervention altered TT performance.

KW - nitrate supplementation

KW - ischemic preconditioning

KW - cycling

UR - http://www.acsmannualmeeting.org/past-meetings/2016-boston/

M3 - Abstract

ER -

McIlvenna LC, Monaghan C, Fernandez BO, Feelisch M, Muggeridge DJ, Easton C. Combined effects of ischemic preconditioning and nitrate supplementation on submaximal cycling exercise and time-trial performance. 2016. Abstract from American College of Sports Medicine Annual Meeting, Boston, MA, United States.