Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations

J.A. Sinclair, C.J. Henderson, I.J. Martin, M.H. Grant, J.N.A. Tettey

Research output: Contribution to journal › Article

Abstract

Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.
LanguageEnglish
PagesA16
JournalJournal of Pharmacy and Pharmacology
Volume59
Issue numberS1
Publication statusPublished - 2007

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Collagenases
Biotransformation
Hepatocytes
Drug-Related Side Effects and Adverse Reactions
Digestion
Suspensions
Perfusion
Enzymes

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Sinclair, J.A. ; Henderson, C.J. ; Martin, I.J. ; Grant, M.H. ; Tettey, J.N.A. / Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations. In: Journal of Pharmacy and Pharmacology. 2007 ; Vol. 59, No. S1. pp. A16.
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Sinclair, JA, Henderson, CJ, Martin, IJ, Grant, MH & Tettey, JNA 2007, 'Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations' Journal of Pharmacy and Pharmacology, vol. 59, no. S1, pp. A16.

Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations. / Sinclair, J.A.; Henderson, C.J.; Martin, I.J.; Grant, M.H.; Tettey, J.N.A.

In: Journal of Pharmacy and Pharmacology, Vol. 59, No. S1, 2007, p. A16.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations

AU - Sinclair, J.A.

AU - Henderson, C.J.

AU - Martin, I.J.

AU - Grant, M.H.

AU - Tettey, J.N.A.

PY - 2007

Y1 - 2007

N2 - Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.

AB - Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.

UR - http://dx.doi.org/10.1211/002235707781850104

M3 - Article

VL - 59

SP - A16

JO - Journal of Pharmacy and Pharmacology

T2 - Journal of Pharmacy and Pharmacology

JF - Journal of Pharmacy and Pharmacology

SN - 0022-3573

IS - S1

ER -

Sinclair JA, Henderson CJ, Martin IJ, Grant MH, Tettey JNA. Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations. Journal of Pharmacy and Pharmacology. 2007;59(S1):A16.

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