Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations

J.A. Sinclair, C.J. Henderson, I.J. Martin, M.H. Grant, J.N.A. Tettey

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Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.
Original languageEnglish
Pages (from-to)A16
JournalJournal of Pharmacy and Pharmacology
Issue numberS1
Publication statusPublished - 2007

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