Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations

J.A. Sinclair, C.J. Henderson, I.J. Martin, M.H. Grant, J.N.A. Tettey

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.
LanguageEnglish
PagesA16
JournalJournal of Pharmacy and Pharmacology
Volume59
Issue numberS1
Publication statusPublished - 2007

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Collagenases
Biotransformation
Hepatocytes
Drug-Related Side Effects and Adverse Reactions
Digestion
Suspensions
Perfusion
Enzymes

Cite this

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Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations. / Sinclair, J.A.; Henderson, C.J.; Martin, I.J.; Grant, M.H.; Tettey, J.N.A.

In: Journal of Pharmacy and Pharmacology, Vol. 59, No. S1, 2007, p. A16.

Research output: Contribution to journalArticle

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AU - Sinclair, J.A.

AU - Henderson, C.J.

AU - Martin, I.J.

AU - Grant, M.H.

AU - Tettey, J.N.A.

PY - 2007

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AB - Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.

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JO - Journal of Pharmacy and Pharmacology

T2 - Journal of Pharmacy and Pharmacology

JF - Journal of Pharmacy and Pharmacology

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