Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations

J.A. Sinclair; C.J. Henderson; I.J. Martin; M.H. Grant; J.N.A. Tettey

Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations

J.A. Sinclair, C.J. Henderson, I.J. Martin, M.H. Grant, J.N.A. Tettey

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.

Original language	English
Pages (from-to)	A16
Journal	Journal of Pharmacy and Pharmacology
Volume	59
Issue number	S1
Publication status	Published - 2007

Cite this

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title = "Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations",

abstract = "Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.",

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T1 - Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations

AU - Sinclair, J.A.

AU - Henderson, C.J.

AU - Martin, I.J.

AU - Grant, M.H.

AU - Tettey, J.N.A.

PY - 2007

Y1 - 2007

N2 - Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.

AB - Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.

UR - http://dx.doi.org/10.1211/002235707781850104

M3 - Article

SN - 0022-3573

VL - 59

SP - A16

JO - Journal of Pharmacy and Pharmacology

JF - Journal of Pharmacy and Pharmacology

IS - S1

ER -

Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations

Abstract

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