Abstract
Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.
| Original language | English |
|---|---|
| Pages (from-to) | A16 |
| Journal | Journal of Pharmacy and Pharmacology |
| Volume | 59 |
| Issue number | S1 |
| Publication status | Published - 2007 |
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Sinclair, J. A., Henderson, C. J., Martin, I. J., Grant, M. H., & Tettey, J. N. A. (2007). Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations. Journal of Pharmacy and Pharmacology, 59(S1), A16.