Abstract
Language | English |
---|---|
Pages | A16 |
Journal | Journal of Pharmacy and Pharmacology |
Volume | 59 |
Issue number | S1 |
Publication status | Published - 2007 |
Fingerprint
Cite this
}
Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations. / Sinclair, J.A.; Henderson, C.J.; Martin, I.J.; Grant, M.H.; Tettey, J.N.A.
In: Journal of Pharmacy and Pharmacology, Vol. 59, No. S1, 2007, p. A16.Research output: Contribution to journal › Article
TY - JOUR
T1 - Collagenase - alone or in combination for the preparation of hepatocytes? The effect on phase 1 and phase 2 biotransformations
AU - Sinclair, J.A.
AU - Henderson, C.J.
AU - Martin, I.J.
AU - Grant, M.H.
AU - Tettey, J.N.A.
PY - 2007
Y1 - 2007
N2 - Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.
AB - Objective: To investigate the effect of digestion enzymes on metabolic biotransformations in suspensions of isolated rat hepatocytes. Isolated rat hepatocytes are a valuable whole cell in vitro tool for drug metabolism and toxicity studies. Several modifications of the original two-stage collagenase perfusion technique (Seglen 1972) have been reported for the preparation of hepatocytes. However, there are very limited published data on the influence of isolation procedures on phase 1 and phase 2 biotransformations in rat hepatocytes.
UR - http://dx.doi.org/10.1211/002235707781850104
M3 - Article
VL - 59
SP - A16
JO - Journal of Pharmacy and Pharmacology
T2 - Journal of Pharmacy and Pharmacology
JF - Journal of Pharmacy and Pharmacology
SN - 0022-3573
IS - S1
ER -