Co–Cr wear products derived from metal-on-metal articulations induce apoptosis on monocyte-like U937 cells

Olga Maria Posada Estefan, Helen Grant, Rothwelle J. Tate

Research output: Contribution to journalMeeting abstract

Abstract

Cobalt–chromium alloy (Co–Cr) particles in the nanometre size range are produced by wear at the articulating surfaces of metal-on-metal implants, and are associated with inflammation, bone loss and implant loosening. Additionally, metal implants undergo corrosion inside the human body releasing ions into the surrounding tissue. This led to the withdrawal from the market of the DePuy ASR™ metal-on-metal hip replacements in 2010. Ions released from Co–Cr particles derived from a resurfacing implant and their subsequent cellular up-take have been measured. Moreover, the ability of the metal debris and Cobalt (Co) ions to induce apoptosis was examined. For this purpose, U937 cells and Co pre-treated U937 cells were exposed to Co, metal debris and Co + debris for 24 and 48 h. To measure metal ion concentrations in culture medium and cell lysates Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis was performed. Apoptosis was evaluated with Fluorescence-Activated Cell Sorting (FACS) and immunoblotting. The Co–Cr wear debris released significant (p < 0.05) amounts of Co and Chromium (Cr) ions into the culture medium, and there was significant (p < 0.05) cellular uptake of both ions when compared to controls: Co ions were taken up to a greater extent than Cr. Furthermore, there was a significant (p < 0.05) increase in apoptosis after 48 h exposure to wear debris. The debris was more effective as an inducer of apoptosis when cells had been pre-treated with Co ions. This suggests that if a patient receives a second Co–Cr implant it may be more likely to produce adverse effects than the first one.
LanguageEnglish
PagesS75
Number of pages1
JournalToxicology Letters
Volume28
Issue numberSuppl
DOIs
Publication statusPublished - Aug 2013
Event49th Congress of the European Societies of Toxicology - EUROTOX 2013 - Interlaken, Switzerland
Duration: 1 Sep 20134 Sep 2013

Fingerprint

Chromium Alloys
Cobalt alloys
Chromium alloys
U937 Cells
Cell death
Cobalt
Monocytes
Debris
Metals
Wear of materials
Ions
Apoptosis
Chromium
Culture Media
Product Recalls and Withdrawals
Inductively coupled plasma mass spectrometry
Osteitis
Corrosion
Sorting
Metal ions

Keywords

  • cobalt–chromium alloy
  • metal on metal articulations
  • metal on metal wear
  • hip replacements

Cite this

@article{034ebd4119ba4886b6c6987791d039c7,
title = "Co–Cr wear products derived from metal-on-metal articulations induce apoptosis on monocyte-like U937 cells",
abstract = "Cobalt–chromium alloy (Co–Cr) particles in the nanometre size range are produced by wear at the articulating surfaces of metal-on-metal implants, and are associated with inflammation, bone loss and implant loosening. Additionally, metal implants undergo corrosion inside the human body releasing ions into the surrounding tissue. This led to the withdrawal from the market of the DePuy ASR™ metal-on-metal hip replacements in 2010. Ions released from Co–Cr particles derived from a resurfacing implant and their subsequent cellular up-take have been measured. Moreover, the ability of the metal debris and Cobalt (Co) ions to induce apoptosis was examined. For this purpose, U937 cells and Co pre-treated U937 cells were exposed to Co, metal debris and Co + debris for 24 and 48 h. To measure metal ion concentrations in culture medium and cell lysates Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis was performed. Apoptosis was evaluated with Fluorescence-Activated Cell Sorting (FACS) and immunoblotting. The Co–Cr wear debris released significant (p < 0.05) amounts of Co and Chromium (Cr) ions into the culture medium, and there was significant (p < 0.05) cellular uptake of both ions when compared to controls: Co ions were taken up to a greater extent than Cr. Furthermore, there was a significant (p < 0.05) increase in apoptosis after 48 h exposure to wear debris. The debris was more effective as an inducer of apoptosis when cells had been pre-treated with Co ions. This suggests that if a patient receives a second Co–Cr implant it may be more likely to produce adverse effects than the first one.",
keywords = "cobalt–chromium alloy, metal on metal articulations, metal on metal wear, hip replacements",
author = "{Posada Estefan}, {Olga Maria} and Helen Grant and Tate, {Rothwelle J.}",
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language = "English",
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pages = "S75",
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Co–Cr wear products derived from metal-on-metal articulations induce apoptosis on monocyte-like U937 cells. / Posada Estefan, Olga Maria; Grant, Helen; Tate, Rothwelle J.

In: Toxicology Letters, Vol. 28, No. Suppl, 08.2013, p. S75.

Research output: Contribution to journalMeeting abstract

TY - JOUR

T1 - Co–Cr wear products derived from metal-on-metal articulations induce apoptosis on monocyte-like U937 cells

AU - Posada Estefan, Olga Maria

AU - Grant, Helen

AU - Tate, Rothwelle J.

PY - 2013/8

Y1 - 2013/8

N2 - Cobalt–chromium alloy (Co–Cr) particles in the nanometre size range are produced by wear at the articulating surfaces of metal-on-metal implants, and are associated with inflammation, bone loss and implant loosening. Additionally, metal implants undergo corrosion inside the human body releasing ions into the surrounding tissue. This led to the withdrawal from the market of the DePuy ASR™ metal-on-metal hip replacements in 2010. Ions released from Co–Cr particles derived from a resurfacing implant and their subsequent cellular up-take have been measured. Moreover, the ability of the metal debris and Cobalt (Co) ions to induce apoptosis was examined. For this purpose, U937 cells and Co pre-treated U937 cells were exposed to Co, metal debris and Co + debris for 24 and 48 h. To measure metal ion concentrations in culture medium and cell lysates Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis was performed. Apoptosis was evaluated with Fluorescence-Activated Cell Sorting (FACS) and immunoblotting. The Co–Cr wear debris released significant (p < 0.05) amounts of Co and Chromium (Cr) ions into the culture medium, and there was significant (p < 0.05) cellular uptake of both ions when compared to controls: Co ions were taken up to a greater extent than Cr. Furthermore, there was a significant (p < 0.05) increase in apoptosis after 48 h exposure to wear debris. The debris was more effective as an inducer of apoptosis when cells had been pre-treated with Co ions. This suggests that if a patient receives a second Co–Cr implant it may be more likely to produce adverse effects than the first one.

AB - Cobalt–chromium alloy (Co–Cr) particles in the nanometre size range are produced by wear at the articulating surfaces of metal-on-metal implants, and are associated with inflammation, bone loss and implant loosening. Additionally, metal implants undergo corrosion inside the human body releasing ions into the surrounding tissue. This led to the withdrawal from the market of the DePuy ASR™ metal-on-metal hip replacements in 2010. Ions released from Co–Cr particles derived from a resurfacing implant and their subsequent cellular up-take have been measured. Moreover, the ability of the metal debris and Cobalt (Co) ions to induce apoptosis was examined. For this purpose, U937 cells and Co pre-treated U937 cells were exposed to Co, metal debris and Co + debris for 24 and 48 h. To measure metal ion concentrations in culture medium and cell lysates Inductively Coupled Plasma Mass Spectrometry (ICP-MS) analysis was performed. Apoptosis was evaluated with Fluorescence-Activated Cell Sorting (FACS) and immunoblotting. The Co–Cr wear debris released significant (p < 0.05) amounts of Co and Chromium (Cr) ions into the culture medium, and there was significant (p < 0.05) cellular uptake of both ions when compared to controls: Co ions were taken up to a greater extent than Cr. Furthermore, there was a significant (p < 0.05) increase in apoptosis after 48 h exposure to wear debris. The debris was more effective as an inducer of apoptosis when cells had been pre-treated with Co ions. This suggests that if a patient receives a second Co–Cr implant it may be more likely to produce adverse effects than the first one.

KW - cobalt–chromium alloy

KW - metal on metal articulations

KW - metal on metal wear

KW - hip replacements

UR - http://www.sciencedirect.com/science/article/pii/S037842741300266X#

U2 - 10.1016/j.toxlet.2013.05.066

DO - 10.1016/j.toxlet.2013.05.066

M3 - Meeting abstract

VL - 28

SP - S75

JO - Toxicology Letters

T2 - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

IS - Suppl

ER -