Clustered integrin α5β1 ligand displays model fibronectin-mediated adhesion of human endometrial stromal cells

Zhaohui Li, Michaela Kreiner, Christopher F van der Walle, Helen J Mardon

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Progress towards endometrial tissue engineering for modelling endometrial diseases and infertility is frustrated by the inability to mimic the fibronectin (FN) extracellular matrix required by human endometrial stromal cells (EnSCs). Here we show that this is because of the requirement to present integrin α5β1 (the FN receptor) ligands in specifically oriented, polyvalent displays; by engineering controlled self-assembly of the 9th-10th type III FN domain pair (FIII9-10, the minimal integrin α5β1 ligand) immobilised in a specific orientation to cell culture surfaces. The fraction of adherent EnSCs seen to spread increased significantly for the multimeric ligand surfaces in the order: tetramer>trimer>dimer>monomer. The extent of EnSC spread morphology also increased in the same order, with the tetrameric ligand supporting a morphology most similar to that supported by FN. Our data suggest that only higher-order multimers of FIII9-10 will fully promote cell spreading mediated through integrin α5β1 binding.
Original languageEnglish
Pages (from-to)777-782
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume407
Issue number4
DOIs
Publication statusPublished - 22 Apr 2011

Keywords

  • cell adhesion
  • endometrium
  • female
  • fibronectins
  • humans
  • Integrin alpha5beta1
  • ligands
  • stromal cells
  • tissue engineering

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