Abstract
A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.
| Language | English |
|---|---|
| Pages | 1243-1247 |
| Number of pages | 5 |
| Journal | Biotechnology Letters |
| Volume | 19 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 1997 |
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Keywords
- protein secondary structures
- fructooligosaccharides
- fructofuranosidase
- oligosaccharides
- prediction
- invertase
- sucrose
- model
Cite this
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Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase. / Somiari, R I ; Brzeski, H ; Tate, R ; Bieleck, S ; Polak, J .
In: Biotechnology Letters, Vol. 19, No. 12, 12.1997, p. 1243-1247.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase
AU - Somiari, R I
AU - Brzeski, H
AU - Tate, R
AU - Bieleck, S
AU - Polak, J
PY - 1997/12
Y1 - 1997/12
N2 - A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.
AB - A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.
KW - protein secondary structures
KW - fructooligosaccharides
KW - fructofuranosidase
KW - oligosaccharides
KW - prediction
KW - invertase
KW - sucrose
KW - model
U2 - 10.1023/A:1018454424640
DO - 10.1023/A:1018454424640
M3 - Article
VL - 19
SP - 1243
EP - 1247
JO - Biotechnology Letters
T2 - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 12
ER -