Abstract
A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.
| Original language | English |
|---|---|
| Pages (from-to) | 1243-1247 |
| Number of pages | 5 |
| Journal | Biotechnology Letters |
| Volume | 19 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 1997 |
Keywords
- protein secondary structures
- fructooligosaccharides
- fructofuranosidase
- oligosaccharides
- prediction
- invertase
- sucrose
- model
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Somiari, R. I., Brzeski, H., Tate, R., Bieleck, S., & Polak, J. (1997). Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase. Biotechnology Letters, 19(12), 1243-1247. https://doi.org/10.1023/A:1018454424640