Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase

R I Somiari, H Brzeski, R Tate, S Bieleck, J Polak

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.

LanguageEnglish
Pages1243-1247
Number of pages5
JournalBiotechnology Letters
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 1997

Fingerprint

beta-Fructofuranosidase
Aspergillus niger
Cloning
Aspergillus
Amino acids
Organism Cloning
DNA
Genes
Reading Frames
Genetic Vectors
Nucleic Acid Databases
Amino Acids
Polymerase Chain Reaction

Keywords

  • protein secondary structures
  • fructooligosaccharides
  • fructofuranosidase
  • oligosaccharides
  • prediction
  • invertase
  • sucrose
  • model

Cite this

Somiari, R I ; Brzeski, H ; Tate, R ; Bieleck, S ; Polak, J . / Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase. In: Biotechnology Letters. 1997 ; Vol. 19, No. 12. pp. 1243-1247.
@article{9aaa9b76576f428daed5931b017acc4d,
title = "Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase",
abstract = "A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.",
keywords = "protein secondary structures, fructooligosaccharides, fructofuranosidase, oligosaccharides, prediction, invertase, sucrose, model",
author = "Somiari, {R I} and H Brzeski and R Tate and S Bieleck and J Polak",
year = "1997",
month = "12",
doi = "10.1023/A:1018454424640",
language = "English",
volume = "19",
pages = "1243--1247",
journal = "Biotechnology Letters",
issn = "0141-5492",
number = "12",

}

Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase. / Somiari, R I ; Brzeski, H ; Tate, R ; Bieleck, S ; Polak, J .

In: Biotechnology Letters, Vol. 19, No. 12, 12.1997, p. 1243-1247.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase

AU - Somiari, R I

AU - Brzeski, H

AU - Tate, R

AU - Bieleck, S

AU - Polak, J

PY - 1997/12

Y1 - 1997/12

N2 - A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.

AB - A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.

KW - protein secondary structures

KW - fructooligosaccharides

KW - fructofuranosidase

KW - oligosaccharides

KW - prediction

KW - invertase

KW - sucrose

KW - model

U2 - 10.1023/A:1018454424640

DO - 10.1023/A:1018454424640

M3 - Article

VL - 19

SP - 1243

EP - 1247

JO - Biotechnology Letters

T2 - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 12

ER -