Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase

R I Somiari, H Brzeski, R Tate, S Bieleck, J Polak

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.

Original languageEnglish
Pages (from-to)1243-1247
Number of pages5
JournalBiotechnology Letters
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 1997

Keywords

  • protein secondary structures
  • fructooligosaccharides
  • fructofuranosidase
  • oligosaccharides
  • prediction
  • invertase
  • sucrose
  • model

Fingerprint Dive into the research topics of 'Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase'. Together they form a unique fingerprint.

  • Cite this