Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase

R I  Somiari; H  Brzeski; R  Tate; S  Bieleck; J  Polak

doi:10.1023/A:1018454424640

Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase

R I Somiari, H Brzeski, R Tate, S Bieleck, J Polak

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.

Original language	English
Pages (from-to)	1243-1247
Number of pages	5
Journal	Biotechnology Letters
Volume	19
Issue number	12
DOIs	https://doi.org/10.1023/A:1018454424640
Publication status	Published - Dec 1997

Keywords

protein secondary structures
fructooligosaccharides
fructofuranosidase
oligosaccharides
prediction
invertase
sucrose
model

Access to Document

10.1023/A:1018454424640

Cite this

@article{9aaa9b76576f428daed5931b017acc4d,

title = "Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase",

abstract = "A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.",

keywords = "protein secondary structures, fructooligosaccharides, fructofuranosidase, oligosaccharides, prediction, invertase, sucrose, model",

author = "Somiari, {R I} and H Brzeski and R Tate and S Bieleck and J Polak",

year = "1997",

month = dec,

doi = "10.1023/A:1018454424640",

language = "English",

volume = "19",

pages = "1243--1247",

journal = "Biotechnology Letters",

issn = "0141-5492",

number = "12",

}

TY - JOUR

T1 - Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase

AU - Somiari, R I

AU - Brzeski, H

AU - Tate, R

AU - Bieleck, S

AU - Polak, J

PY - 1997/12

Y1 - 1997/12

N2 - A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.

AB - A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.

KW - protein secondary structures

KW - fructooligosaccharides

KW - fructofuranosidase

KW - oligosaccharides

KW - prediction

KW - invertase

KW - sucrose

KW - model

U2 - 10.1023/A:1018454424640

DO - 10.1023/A:1018454424640

M3 - Article

VL - 19

SP - 1243

EP - 1247

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 12

ER -

Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase

Abstract

Keywords

Access to Document

Fingerprint

Cite this