Circulating human B lymphocytes are deficient in nucleotide excision repair and accumulate mutations upon proliferation

Nevila Hyka-Nouspikel, Kimon Lemonidis, Wei-Ting Lu, Thierry Nouspikel

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Faithful repair of DNA lesions is a crucial task that dividing cells must actively perform to maintain genome integrity. Strikingly, nucleotide excision repair (NER), the most versatile DNA repair system, is specifically down-regulated in terminally differentiated cells. This prompted us to examine whether NER attenuation might be a common feature of all G0-arrested cells, and in particular of those that retain the capacity to reenter cell cycle and might thus convert unrepaired DNA lesions into mutations, a prerequisite for malignant transformation. Here we report that quiescent primary human B lymphocytes down-regulate NER at the global genome level while maintaining proficient repair of constitutively expressed genes. Quiescent B cells exposed to an environment that causes both DNA damage and proliferation accumulate point mutations in silent and inducible genes crucial for cell replication and differentiation, such as BCL6 and Cyclin D2. Similar to differentiated cells, NER attenuation in quiescent cells is associated with incomplete phosphorylation of the ubiquitin activating enzyme Ube1, which is required for proficient NER. Our data establish a mechanistic link between NER attenuation during quiescence and cell mutagenesis and also support the concept that oncogenic events targeting cell cycle- or activation-induced genes might initiate genomic instability and lymphomagenesis.
Original languageEnglish
Pages (from-to)6277-6286
Number of pages11
JournalBlood
Early online date8 Apr 2011
DOIs
Publication statusPublished - 2011

Fingerprint

Lymphocytes
DNA Repair
Repair
B-Lymphocytes
Nucleotides
Mutation
Genes
DNA
Cells
Cell Cycle
Ubiquitin-Activating Enzymes
Cyclin D2
Genome
Genomic Instability
Mutagenesis
Phosphorylation
Point Mutation
Transcriptional Activation
DNA Damage
Cell Differentiation

Keywords

  • circulating
  • human B lymphocytes
  • nucleotide excision repair
  • mutations
  • proliferation
  • DNA lesions
  • repair
  • NER

Cite this

Hyka-Nouspikel, Nevila ; Lemonidis, Kimon ; Lu, Wei-Ting ; Nouspikel, Thierry. / Circulating human B lymphocytes are deficient in nucleotide excision repair and accumulate mutations upon proliferation. In: Blood. 2011 ; pp. 6277-6286.
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Circulating human B lymphocytes are deficient in nucleotide excision repair and accumulate mutations upon proliferation. / Hyka-Nouspikel, Nevila; Lemonidis, Kimon; Lu, Wei-Ting; Nouspikel, Thierry.

In: Blood, 2011, p. 6277-6286.

Research output: Contribution to journalArticle

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