Chromosome segregation during Streptomyces coelicolor differrentiation

Graham Edward Falconer

Research output: ThesisMaster's Thesis

Abstract

In Streptomyces coelicolor DNA segregation is, in part, controlled by the proteins ParA and ParB. Segregation is achieved by ParB binding to parS sites, located near oriC on the bacterial chromosome, and ParA providing the separation energy for segregation. Several mutant strains containing a fluorescently tagged oriC and deletions in parA or parB were created and their growth and positioning of chromosomes, as seen by fluorescent microscopy, were studied over the different stages of its life cycle. Here I show the various differences caused by these mutations within S. coelicolor and highlight potential roles of the Par proteins in dictating the size of spores
LanguageEnglish
QualificationMPhil
Awarding Institution
  • University Of Strathclyde
Supervisors/Advisors
  • Herron, Paul, Supervisor
Award date27 Oct 2014
Place of PublicationGlasgow
Publisher
Publication statusPublished - 27 Oct 2014

Fingerprint

Streptomyces coelicolor
Chromosome Segregation
Chromosome Positioning
Bacterial Chromosomes
Life Cycle Stages
Spores
Microscopy
Proteins
Mutation
DNA
Growth

Keywords

  • streptomyces
  • chromosome segregation

Cite this

Falconer, G. E. (2014). Chromosome segregation during Streptomyces coelicolor differrentiation. Glasgow: University of Strathclyde.
Falconer, Graham Edward. / Chromosome segregation during Streptomyces coelicolor differrentiation. Glasgow : University of Strathclyde, 2014.
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Falconer, GE 2014, 'Chromosome segregation during Streptomyces coelicolor differrentiation', MPhil, University Of Strathclyde, Glasgow.

Chromosome segregation during Streptomyces coelicolor differrentiation. / Falconer, Graham Edward.

Glasgow : University of Strathclyde, 2014.

Research output: ThesisMaster's Thesis

TY - THES

T1 - Chromosome segregation during Streptomyces coelicolor differrentiation

AU - Falconer, Graham Edward

PY - 2014/10/27

Y1 - 2014/10/27

N2 - In Streptomyces coelicolor DNA segregation is, in part, controlled by the proteins ParA and ParB. Segregation is achieved by ParB binding to parS sites, located near oriC on the bacterial chromosome, and ParA providing the separation energy for segregation. Several mutant strains containing a fluorescently tagged oriC and deletions in parA or parB were created and their growth and positioning of chromosomes, as seen by fluorescent microscopy, were studied over the different stages of its life cycle. Here I show the various differences caused by these mutations within S. coelicolor and highlight potential roles of the Par proteins in dictating the size of spores

AB - In Streptomyces coelicolor DNA segregation is, in part, controlled by the proteins ParA and ParB. Segregation is achieved by ParB binding to parS sites, located near oriC on the bacterial chromosome, and ParA providing the separation energy for segregation. Several mutant strains containing a fluorescently tagged oriC and deletions in parA or parB were created and their growth and positioning of chromosomes, as seen by fluorescent microscopy, were studied over the different stages of its life cycle. Here I show the various differences caused by these mutations within S. coelicolor and highlight potential roles of the Par proteins in dictating the size of spores

KW - streptomyces

KW - chromosome segregation

M3 - Master's Thesis

PB - University of Strathclyde

CY - Glasgow

ER -

Falconer GE. Chromosome segregation during Streptomyces coelicolor differrentiation. Glasgow: University of Strathclyde, 2014.