Chromium VI induced damage to the cytoskeleton and cell death in isolated hepatocytes

M. Gunaratnam, M.H. Grant

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Cr(VI) is a known human carcinogen. Although it has been investigated widely, the mechanism(s) of its action is/are not fully understood. The aim of this study was to evaluate Cr(VI)-induced damage to the cell cytoskeleton and the mode of cell death in primary cultures of hepatocytes. Exposure of the cultured cells (105/cm2) to 1 and 5 µM Cr(VI) for 24 h resulted in loss of the cell cytoskeleton, and this was accompanied by membrane blebbing and shrinking of the cell. Staining of the cells with annexin V and propidium iodide showed that Cr(VI) induces apoptosis at low concentrations (5 µM), whereas at higher concentrations (25 µM) it induces necrosis. This study shows that Cr(VI) causes damage to the cell cytoskeleton, and induces apoptosis at low concentrations. However, the importance of necrosis and apoptosis in vivo, and the effects of longer exposure times, which simulate environmental and occupational exposure to Cr(VI), remain to be investigated.
    LanguageEnglish
    Pages748-750
    Number of pages2
    JournalBiochemical Society Transactions
    Volume30
    Publication statusPublished - 2002

    Fingerprint

    Cell death
    Cytoskeleton
    Hepatocytes
    Cell Death
    Apoptosis
    Necrosis
    Propidium
    Annexin A5
    Environmental Exposure
    Occupational Exposure
    Blister
    Cell culture
    Carcinogens
    chromium hexavalent ion
    Cultured Cells
    Cells
    Staining and Labeling
    Membranes

    Keywords

    • apoptosis
    • Cr(VI)
    • cultured hepatocytes
    • bioengineering

    Cite this

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    abstract = "Cr(VI) is a known human carcinogen. Although it has been investigated widely, the mechanism(s) of its action is/are not fully understood. The aim of this study was to evaluate Cr(VI)-induced damage to the cell cytoskeleton and the mode of cell death in primary cultures of hepatocytes. Exposure of the cultured cells (105/cm2) to 1 and 5 µM Cr(VI) for 24 h resulted in loss of the cell cytoskeleton, and this was accompanied by membrane blebbing and shrinking of the cell. Staining of the cells with annexin V and propidium iodide showed that Cr(VI) induces apoptosis at low concentrations (5 µM), whereas at higher concentrations (25 µM) it induces necrosis. This study shows that Cr(VI) causes damage to the cell cytoskeleton, and induces apoptosis at low concentrations. However, the importance of necrosis and apoptosis in vivo, and the effects of longer exposure times, which simulate environmental and occupational exposure to Cr(VI), remain to be investigated.",
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    Chromium VI induced damage to the cytoskeleton and cell death in isolated hepatocytes. / Gunaratnam, M.; Grant, M.H.

    In: Biochemical Society Transactions, Vol. 30, 2002, p. 748-750.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Chromium VI induced damage to the cytoskeleton and cell death in isolated hepatocytes

    AU - Gunaratnam, M.

    AU - Grant, M.H.

    PY - 2002

    Y1 - 2002

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    AB - Cr(VI) is a known human carcinogen. Although it has been investigated widely, the mechanism(s) of its action is/are not fully understood. The aim of this study was to evaluate Cr(VI)-induced damage to the cell cytoskeleton and the mode of cell death in primary cultures of hepatocytes. Exposure of the cultured cells (105/cm2) to 1 and 5 µM Cr(VI) for 24 h resulted in loss of the cell cytoskeleton, and this was accompanied by membrane blebbing and shrinking of the cell. Staining of the cells with annexin V and propidium iodide showed that Cr(VI) induces apoptosis at low concentrations (5 µM), whereas at higher concentrations (25 µM) it induces necrosis. This study shows that Cr(VI) causes damage to the cell cytoskeleton, and induces apoptosis at low concentrations. However, the importance of necrosis and apoptosis in vivo, and the effects of longer exposure times, which simulate environmental and occupational exposure to Cr(VI), remain to be investigated.

    KW - apoptosis

    KW - Cr(VI)

    KW - cultured hepatocytes

    KW - bioengineering

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    M3 - Article

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    JO - Biochemical Society Transactions

    T2 - Biochemical Society Transactions

    JF - Biochemical Society Transactions

    SN - 0300-5127

    ER -