TY - JOUR
T1 - Chromatographic comparison of bupivacaine imprinted polymers prepared in different formats: crushed monolith, microsphere, silica-based composite and capillary monolith formats
AU - Cormack, Peter
AU - J. Oxelbark, C. Legido-Quigley, C.S.A. Aureliano, M.-M. Titirici, E. Schillinger, B. Sellergren, J. Courtois, K. Irgum, L. Dambies, P.A.G. Cormack, D.C. Sherrington and E. De Lorenzi
PY - 2007
Y1 - 2007
N2 - A comprehensive comparison of five chromatographic stationary phases based on molecularly imprinted polymers is presented. Efficiency, imprinting factors, water compatibility and batch-to-batch reproducibility are discussed for crushed monolith, microspheres, two silica-based composites and capillary monoliths, all imprinted with the local anaesthetic bupivacaine. Synthesis protocol and chromatographic test conditions have been kept fixed within certain limits, in order to provide further insight into the strengths and weaknesses of the different formats. Excluding microparticles, all formats give satisfactory performance, especially in aqueous mobile phases. An assessment of batch-to-batch reproducibility in different mobile phases adds further value to this comparison study.
AB - A comprehensive comparison of five chromatographic stationary phases based on molecularly imprinted polymers is presented. Efficiency, imprinting factors, water compatibility and batch-to-batch reproducibility are discussed for crushed monolith, microspheres, two silica-based composites and capillary monoliths, all imprinted with the local anaesthetic bupivacaine. Synthesis protocol and chromatographic test conditions have been kept fixed within certain limits, in order to provide further insight into the strengths and weaknesses of the different formats. Excluding microparticles, all formats give satisfactory performance, especially in aqueous mobile phases. An assessment of batch-to-batch reproducibility in different mobile phases adds further value to this comparison study.
KW - adsorption
KW - bupivacaine
KW - chromatography
KW - microspheres
KW - polymers
KW - porosity silicon dioxide
U2 - 10.1016/j.chroma.2007.05.057
DO - 10.1016/j.chroma.2007.05.057
M3 - Article
SP - 215
EP - 226
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
ER -