Chromatographic comparison of bupivacaine imprinted polymers prepared in different formats: crushed monolith, microsphere, silica-based composite and capillary monolith formats

J. Oxelbark, C. Legido-Quigley, C.S.A. Aureliano, M.-M. Titirici, E. Schillinger, B. Sellergren, J. Courtois, K. Irgum, L. Dambies, P.A.G. Cormack, D.C. Sherrington and E. De Lorenzi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

A comprehensive comparison of five chromatographic stationary phases based on molecularly imprinted polymers is presented. Efficiency, imprinting factors, water compatibility and batch-to-batch reproducibility are discussed for crushed monolith, microspheres, two silica-based composites and capillary monoliths, all imprinted with the local anaesthetic bupivacaine. Synthesis protocol and chromatographic test conditions have been kept fixed within certain limits, in order to provide further insight into the strengths and weaknesses of the different formats. Excluding microparticles, all formats give satisfactory performance, especially in aqueous mobile phases. An assessment of batch-to-batch reproducibility in different mobile phases adds further value to this comparison study.
Original languageEnglish
Pages (from-to)215-226
Number of pages12
JournalJournal of Chromatography A
DOIs
Publication statusPublished - 2007

Keywords

  • adsorption
  • bupivacaine
  • chromatography
  • microspheres
  • polymers
  • porosity silicon dioxide

Fingerprint Dive into the research topics of 'Chromatographic comparison of bupivacaine imprinted polymers prepared in different formats: crushed monolith, microsphere, silica-based composite and capillary monolith formats'. Together they form a unique fingerprint.

  • Cite this

    J. Oxelbark, C. Legido-Quigley, C.S.A. Aureliano, M.-M. Titirici, E. Schillinger, B. Sellergren, J. Courtois, K. Irgum, L. Dambies, P.A.G. Cormack, D.C. Sherrington and E. De Lorenzi (2007). Chromatographic comparison of bupivacaine imprinted polymers prepared in different formats: crushed monolith, microsphere, silica-based composite and capillary monolith formats. Journal of Chromatography A , 215-226. https://doi.org/10.1016/j.chroma.2007.05.057