Chiral analysis of methylphenidate and dextromoramide by capillary electrophoresis

Oliver M. Denk, D.G. Watson, G.G. Skellern

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Capillary electrophoretic methods have been developed to separate the enantiomers of methylphenidate (MPH) and dextromoramide. For MPH separation was achieved with heptakis (2,6-di-O-methyl)-beta -cyclodextrin (DMCD) as chiral selector in a 100 mM phosphoric acid buffer adjusted to pH 3.0 with triethanolamine. Commercial samples Of D,L-erytho-MPH HCl and D,L-threo-MPH HCl were analysed using the method. There was no evidence of the presence of D,L-threo-MPH HCl in D,L-etytho-MPH HCl and vice versa. The ratio of the enantiomers was determined for each diasteroisomer. Hydroxypropyl-beta -cyclodextrin was the chiral selector of choice for the chiral separation of the enantiomers of moramide. The separation which gave a resolution of about 3.5 was achieved in 4 min using only a 6 cm of length of capillary. In a sample of dextro-R-moramide tartrate only a small quantity (4.9% w/w) of levo-S-moramide was detected with this method.
Original languageEnglish
Pages (from-to)61-68
Number of pages8
JournalJournal of chromatography. B, Biomedical sciences and applications
Volume761
Issue number1
DOIs
Publication statusPublished - 15 Sep 2001

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Dextromoramide
Capillary electrophoresis
Methylphenidate
Enantiomers
Buffers

Keywords

  • enantiomer separation
  • methylphenidate
  • dextromoramide

Cite this

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abstract = "Capillary electrophoretic methods have been developed to separate the enantiomers of methylphenidate (MPH) and dextromoramide. For MPH separation was achieved with heptakis (2,6-di-O-methyl)-beta -cyclodextrin (DMCD) as chiral selector in a 100 mM phosphoric acid buffer adjusted to pH 3.0 with triethanolamine. Commercial samples Of D,L-erytho-MPH HCl and D,L-threo-MPH HCl were analysed using the method. There was no evidence of the presence of D,L-threo-MPH HCl in D,L-etytho-MPH HCl and vice versa. The ratio of the enantiomers was determined for each diasteroisomer. Hydroxypropyl-beta -cyclodextrin was the chiral selector of choice for the chiral separation of the enantiomers of moramide. The separation which gave a resolution of about 3.5 was achieved in 4 min using only a 6 cm of length of capillary. In a sample of dextro-R-moramide tartrate only a small quantity (4.9{\%} w/w) of levo-S-moramide was detected with this method.",
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Chiral analysis of methylphenidate and dextromoramide by capillary electrophoresis. / Denk, Oliver M.; Watson, D.G.; Skellern, G.G.

In: Journal of chromatography. B, Biomedical sciences and applications , Vol. 761, No. 1, 15.09.2001, p. 61-68.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chiral analysis of methylphenidate and dextromoramide by capillary electrophoresis

AU - Denk, Oliver M.

AU - Watson, D.G.

AU - Skellern, G.G.

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AB - Capillary electrophoretic methods have been developed to separate the enantiomers of methylphenidate (MPH) and dextromoramide. For MPH separation was achieved with heptakis (2,6-di-O-methyl)-beta -cyclodextrin (DMCD) as chiral selector in a 100 mM phosphoric acid buffer adjusted to pH 3.0 with triethanolamine. Commercial samples Of D,L-erytho-MPH HCl and D,L-threo-MPH HCl were analysed using the method. There was no evidence of the presence of D,L-threo-MPH HCl in D,L-etytho-MPH HCl and vice versa. The ratio of the enantiomers was determined for each diasteroisomer. Hydroxypropyl-beta -cyclodextrin was the chiral selector of choice for the chiral separation of the enantiomers of moramide. The separation which gave a resolution of about 3.5 was achieved in 4 min using only a 6 cm of length of capillary. In a sample of dextro-R-moramide tartrate only a small quantity (4.9% w/w) of levo-S-moramide was detected with this method.

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