Chemical and antimicrobial profiling of propolis from different regions within Libya

Weam Siheri, Tong Zhang, Godwin Unekwuojo Ebiloma, Marco Biddau, Nicola Woods, Muattaz Yassein Hussain, Carol J. Clements, James Fearnley, RuAngelie Edrada Ebel, Timothy Paget, Sylke Müller, Katharine C. Carter, Valerie A. Ferro, Harry P. De Koning, David G. Watson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Extracts from twelve samples of propolis collected from different regions of Libya were tested for their activity against Trypanosoma brucei, Leishmania donovani, Plasmodium falciparum, Crithidia fasciculata and Mycobacterium marinum and the cytotoxicity of the extracts was tested against mammalian cells. All the extracts were active to some degree against all of the protozoa and the mycobacterium, exhibiting a range of EC50 values between 1.65 and 53.6 μg/ml. The toxicity against mammalian cell lines was only moderate; the most active extract against the protozoan species, P2, displayed an IC50 value of 53.2 μg/ml. The extracts were profiled by using liquid chromatography coupled to high resolution mass spectrometry. The data sets were extracted using m/z Mine and the accurate masses of the features extracted were searched against the Dictionary of Natural Products (DNP). A principal component analysis (PCA) model was constructed which, in combination with hierarchical cluster analysis (HCA), divided the samples into five groups. The outlying groups had different sets of dominant compounds in the extracts, which could be characterised by their elemental composition. Orthogonal partial least squares (OPLS) analysis was used to link the activity of each extract against the different micro-organisms to particular components in the extracts.
LanguageEnglish
Article numbere0155355
Number of pages16
JournalPLOS One
Volume11
Issue number5
Early online date19 May 2016
DOIs
Publication statusPublished - 19 May 2016

Fingerprint

Crithidia fasciculata
Mycobacterium marinum
Libya
Propolis
Leishmania donovani
Trypanosoma brucei brucei
propolis
Plasmodium falciparum
Mycobacterium
Principal Component Analysis
Biological Products
Least-Squares Analysis
Liquid Chromatography
Inhibitory Concentration 50
Cluster Analysis
Mass Spectrometry
anti-infective agents
Cells
Protozoa
Cell Line

Keywords

  • bees
  • isomers
  • Libya
  • toxicity
  • Leishmania donovani
  • protozoans

Cite this

Siheri, Weam ; Zhang, Tong ; Ebiloma, Godwin Unekwuojo ; Biddau, Marco ; Woods, Nicola ; Hussain, Muattaz Yassein ; Clements, Carol J. ; Fearnley, James ; Edrada Ebel, RuAngelie ; Paget, Timothy ; Müller, Sylke ; Carter, Katharine C. ; Ferro, Valerie A. ; De Koning, Harry P. ; Watson, David G. / Chemical and antimicrobial profiling of propolis from different regions within Libya. In: PLOS One. 2016 ; Vol. 11, No. 5.
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Siheri, W, Zhang, T, Ebiloma, GU, Biddau, M, Woods, N, Hussain, MY, Clements, CJ, Fearnley, J, Edrada Ebel, R, Paget, T, Müller, S, Carter, KC, Ferro, VA, De Koning, HP & Watson, DG 2016, 'Chemical and antimicrobial profiling of propolis from different regions within Libya' PLOS One, vol. 11, no. 5, e0155355. https://doi.org/10.1371/journal.pone.0155355

Chemical and antimicrobial profiling of propolis from different regions within Libya. / Siheri, Weam; Zhang, Tong; Ebiloma, Godwin Unekwuojo; Biddau, Marco; Woods, Nicola; Hussain, Muattaz Yassein; Clements, Carol J.; Fearnley, James; Edrada Ebel, RuAngelie; Paget, Timothy; Müller, Sylke; Carter, Katharine C.; Ferro, Valerie A.; De Koning, Harry P.; Watson, David G.

In: PLOS One, Vol. 11, No. 5, e0155355, 19.05.2016.

Research output: Contribution to journalArticle

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T1 - Chemical and antimicrobial profiling of propolis from different regions within Libya

AU - Siheri, Weam

AU - Zhang, Tong

AU - Ebiloma, Godwin Unekwuojo

AU - Biddau, Marco

AU - Woods, Nicola

AU - Hussain, Muattaz Yassein

AU - Clements, Carol J.

AU - Fearnley, James

AU - Edrada Ebel, RuAngelie

AU - Paget, Timothy

AU - Müller, Sylke

AU - Carter, Katharine C.

AU - Ferro, Valerie A.

AU - De Koning, Harry P.

AU - Watson, David G.

PY - 2016/5/19

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N2 - Extracts from twelve samples of propolis collected from different regions of Libya were tested for their activity against Trypanosoma brucei, Leishmania donovani, Plasmodium falciparum, Crithidia fasciculata and Mycobacterium marinum and the cytotoxicity of the extracts was tested against mammalian cells. All the extracts were active to some degree against all of the protozoa and the mycobacterium, exhibiting a range of EC50 values between 1.65 and 53.6 μg/ml. The toxicity against mammalian cell lines was only moderate; the most active extract against the protozoan species, P2, displayed an IC50 value of 53.2 μg/ml. The extracts were profiled by using liquid chromatography coupled to high resolution mass spectrometry. The data sets were extracted using m/z Mine and the accurate masses of the features extracted were searched against the Dictionary of Natural Products (DNP). A principal component analysis (PCA) model was constructed which, in combination with hierarchical cluster analysis (HCA), divided the samples into five groups. The outlying groups had different sets of dominant compounds in the extracts, which could be characterised by their elemental composition. Orthogonal partial least squares (OPLS) analysis was used to link the activity of each extract against the different micro-organisms to particular components in the extracts.

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KW - bees

KW - isomers

KW - Libya

KW - toxicity

KW - Leishmania donovani

KW - protozoans

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DO - 10.1371/journal.pone.0155355

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