Metallic nanoparticles can be used as basic materials for a wide variety of purposes including building blocks for nanoassemblies, substrates for enhanced spectroscopies such as fluorescence and Raman, and as labels for biomolecules. Here we report how silver and gold nanoparticles can be functionalized with specific biomolecular probes to indicate the molecular recognition of a target molecule. Examples of this approach include DNA hybridization to switch on surface enhanced resonance Raman scattering (SERRS) when a specific target sequence is present, recognition of specific proteins by aptamer functionalised nanoparticles through surface plasmon resonance or SERRS, and the use of nanoparticles functionalized with antibodies to provide a new type of immunoassay. These examples indicate how nanoparticles can be used to provide highly sensitive and informative data from a variety of biological systems when used with optical spectroscopy.
|Number of pages||1|
|Journal||Abstracts of papers - American Chemical Society|
|Publication status||Published - 16 Aug 2009|