CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis

Stéphane M. Camus, Marine D. Camus, Carmen Figueras-Novoa, Gaelle Boncompain, L. Amanda Sadacca, Christopher Esk, Anne Bigot, Gwyn W. Gould, Dimitrios Kioumourtzoglou, Franck Perez, Nia J. Bryant, Shaeri Mukherjee, Frances M. Brodsky

Research output: Contribution to journalArticle

Abstract

Post-prandial blood glucose is cleared by Glucose Transporter 4 (GLUT4) released from an intracellular GLUT4 storage compartment (GSC) to the surface of muscle and adipose tissue in response to insulin. Here we map the biosynthetic pathway for human GSC formation, which involves the clathrin isoform CHC22. We observe that GLUT4 transits more slowly through the early secretory pathway than the constitutively-secreted GLUT1 transporter, and show CHC22 colocalizes with p115 in the endoplasmic-reticulum-to-Golgi-intermediate compartment (ERGIC). We find CHC22 functions in membrane traffic from the early secretory pathway during formation of the replication vacuole of Legionella pneumophila, which also acquires components of the GLUT4 pathway. We show that p115 but not GM130 is required for GSC formation, indicating GSC biogenesis from the ERGIC bypasses the Golgi. This GSC biogenesis pathway is attenuated in mice, which lack CHC22, and rely mainly on recapture of surface GLUT4 to populate their GSC. GLUT4 traffic to the GSC is enhanced by CHC22 function at the human ERGIC, which has implications for pathways to insulin resistance.
LanguageEnglish
Number of pages52
JournalJournal of Cell Biology
Publication statusAccepted/In press - 9 Oct 2019

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Clathrin
Facilitative Glucose Transport Proteins
Endoplasmic Reticulum
Secretory Pathway
Legionella pneumophila
Biosynthetic Pathways
Vacuoles
Meals
Insulin Resistance
Blood Glucose
Adipose Tissue
Protein Isoforms
Insulin
Muscles
Membranes

Keywords

  • glucose transporter 4
  • insulin
  • diabetes
  • membrane trafficking

Cite this

Camus, S. M., Camus, M. D., Figueras-Novoa, C., Boncompain, G., Sadacca, L. A., Esk, C., ... Brodsky, F. M. (Accepted/In press). CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis. Journal of Cell Biology.
Camus, Stéphane M. ; Camus, Marine D. ; Figueras-Novoa, Carmen ; Boncompain, Gaelle ; Sadacca, L. Amanda ; Esk, Christopher ; Bigot, Anne ; Gould, Gwyn W. ; Kioumourtzoglou, Dimitrios ; Perez, Franck ; Bryant, Nia J. ; Mukherjee, Shaeri ; Brodsky, Frances M. / CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis. In: Journal of Cell Biology. 2019.
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Camus, SM, Camus, MD, Figueras-Novoa, C, Boncompain, G, Sadacca, LA, Esk, C, Bigot, A, Gould, GW, Kioumourtzoglou, D, Perez, F, Bryant, NJ, Mukherjee, S & Brodsky, FM 2019, 'CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis' Journal of Cell Biology.

CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis. / Camus, Stéphane M.; Camus, Marine D.; Figueras-Novoa, Carmen; Boncompain, Gaelle; Sadacca, L. Amanda; Esk, Christopher; Bigot, Anne; Gould, Gwyn W.; Kioumourtzoglou, Dimitrios; Perez, Franck; Bryant, Nia J.; Mukherjee, Shaeri; Brodsky, Frances M.

In: Journal of Cell Biology, 09.10.2019.

Research output: Contribution to journalArticle

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AU - Camus, Stéphane M.

AU - Camus, Marine D.

AU - Figueras-Novoa, Carmen

AU - Boncompain, Gaelle

AU - Sadacca, L. Amanda

AU - Esk, Christopher

AU - Bigot, Anne

AU - Gould, Gwyn W.

AU - Kioumourtzoglou, Dimitrios

AU - Perez, Franck

AU - Bryant, Nia J.

AU - Mukherjee, Shaeri

AU - Brodsky, Frances M.

PY - 2019/10/9

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N2 - Post-prandial blood glucose is cleared by Glucose Transporter 4 (GLUT4) released from an intracellular GLUT4 storage compartment (GSC) to the surface of muscle and adipose tissue in response to insulin. Here we map the biosynthetic pathway for human GSC formation, which involves the clathrin isoform CHC22. We observe that GLUT4 transits more slowly through the early secretory pathway than the constitutively-secreted GLUT1 transporter, and show CHC22 colocalizes with p115 in the endoplasmic-reticulum-to-Golgi-intermediate compartment (ERGIC). We find CHC22 functions in membrane traffic from the early secretory pathway during formation of the replication vacuole of Legionella pneumophila, which also acquires components of the GLUT4 pathway. We show that p115 but not GM130 is required for GSC formation, indicating GSC biogenesis from the ERGIC bypasses the Golgi. This GSC biogenesis pathway is attenuated in mice, which lack CHC22, and rely mainly on recapture of surface GLUT4 to populate their GSC. GLUT4 traffic to the GSC is enhanced by CHC22 function at the human ERGIC, which has implications for pathways to insulin resistance.

AB - Post-prandial blood glucose is cleared by Glucose Transporter 4 (GLUT4) released from an intracellular GLUT4 storage compartment (GSC) to the surface of muscle and adipose tissue in response to insulin. Here we map the biosynthetic pathway for human GSC formation, which involves the clathrin isoform CHC22. We observe that GLUT4 transits more slowly through the early secretory pathway than the constitutively-secreted GLUT1 transporter, and show CHC22 colocalizes with p115 in the endoplasmic-reticulum-to-Golgi-intermediate compartment (ERGIC). We find CHC22 functions in membrane traffic from the early secretory pathway during formation of the replication vacuole of Legionella pneumophila, which also acquires components of the GLUT4 pathway. We show that p115 but not GM130 is required for GSC formation, indicating GSC biogenesis from the ERGIC bypasses the Golgi. This GSC biogenesis pathway is attenuated in mice, which lack CHC22, and rely mainly on recapture of surface GLUT4 to populate their GSC. GLUT4 traffic to the GSC is enhanced by CHC22 function at the human ERGIC, which has implications for pathways to insulin resistance.

KW - glucose transporter 4

KW - insulin

KW - diabetes

KW - membrane trafficking

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M3 - Article

JO - Journal of Cell Biology

T2 - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

ER -

Camus SM, Camus MD, Figueras-Novoa C, Boncompain G, Sadacca LA, Esk C et al. CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesis. Journal of Cell Biology. 2019 Oct 9.